microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2019-2-50-54microbe-1148Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВлияние специфических бактериофагов и гентамицина на морфологию и везикулообразование бактерий Yersinia pestis EVEffect of Bacteriophages and Gentamycine on Morphology and Vesicle Formation of Bacteria Yersinia pestis EV ДудинаЛ. Г.DudinaL. G.

610000, Киров, Московская ул., 36;

Сыктывкар

36, Moskovskaya St., Kirov, 610000;

Syktyvkar

necdew@mail.ru МалковаМ. А.MalkovaM. A.

610000, Киров, Московская ул., 36;

Сыктывкар

36, Moskovskaya St., Kirov, 610000;

Syktyvkar

info@vyatsu.ruЧернядьевА. В.Chernyad’evA. V.

610000, Киров, Московская ул., 36

36, Moskovskaya St., Kirov, 610000

info@vyatsu.ru ЛитвинецС. Г.LitvinetsS. G.

610000, Киров, Московская ул., 36

36, Moskovskaya St., Kirov, 610000

info@vyatsu.ru БываловА. А.ByvalovA. A.

610000, Киров, Московская ул., 36;

Сыктывкар

36, Moskovskaya St., Kirov, 610000;

Syktyvkar

info@vyatsu.ruВятский государственный университет; Институт физиологии Коми НЦ УрО РАНРоссияVyatka State University; Komi Institute of Physiology at the Scientific Center of the Ural Branch of RASRussian FederationВятский государственный университетРоссияVyatka State UniversityRussian Federation201901072019025054Copyright ©  Дудина Л.Г.,  Малкова М.А., Чернядьев А.В.,  Литвинец С.Г.,  Бывалов А.А., 20192019 Дудина Л.Г.,  Малкова М.А., Чернядьев А.В.,  Литвинец С.Г.,  Бывалов А.А.Dudina L.G., Malkova M.A., Chernyad’ev A.V., Litvinets S.G., Byvalov A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/1148

Цель – оценить влияние специфических бактериофагов и гентамицина на морфо-функциональные свойства бактерий вакцинного штамма Yersinia pestis EV.

Материалы и методы

Материалы и методы. В работе использовали вакцинный штамм Y. pestis EV линии НИИЭГ, бактериофаги чумной Покровской и псевдотуберкулезный диагностический. Микробную культуру выращивали на плотной и в жидкой питательных средах при температуре 27 °С в течение 20–24 ч. Совместную инкубацию бактерий и бактериофага или гентамицина проводили при температуре 27 °С в течение 20 мин или 37 °С в течение 2 ч соответственно. Препараты культур исследовали методом просвечивающей электронной микроскопии.

Результаты и обсуждение

Результаты и обсуждение. Проведена оценка влияния условий культивирования и различных стрессорных факторов на процесс везикулообразования клетками вакцинного штамма Y. pestis EV. Определены характер и выраженность морфофункциональных изменений в клетках Y. pestis EV в ответ на воздействие бактериофагами (чумной Покровской и псевдотуберкулезный) или антибиотиком (гентамицин). Установлено, что совместная инкубация в течение 20 мин Y. pestis EV с бактериофагом Покровской или гентамицином приводит к увеличению продукции внеклеточных везикул и сопровождается развитием дегенеративных изменений бактериальных клеток. 

Objective was to assess the effect of specific bacteriophages and gentamycine on the morphological-functional properties of bacteria in the vaccine strain Yersinia pestis EV.

Materials and methods

Materials and methods. The vaccine strain Y. pestis EV, Pokrovskaya bacteriophage and the pseudotuberculous diagnostic bacteriophage were used for the study. The microbial culture was grown on solid and in liquid growth media at 27 °C for 20–24 h. The co-incubation of bacteria and bacteriophage or gentamycine was carried out at 27 °C for 20 minutes or at 37 °C for 2 hours, respectively. Culture preparations were examined by transmission electron microscopy.

Results and discussion

Results and discussion. The influence of cultivation conditions and various stress factors on the vesicle production by the vaccine strain Y. pestis EV cells was evaluated. The nature and intensity of morphological-functional changes in Y. pestis EV cells in response to the effect of bacteriophages (plague Pokrovskaya and pseudotuberculous bacteriophages) or an antibiotic (gentamycine) were determined. It was established that co-incubation of Y. pestis EV with Pokrovskaya bacteriophage or gentamycine for 20 min leads to the increase in the production of extracellular vesicles and is accompanied by the development of degenerative changes in bacterial cells. 

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The authors declare that there are no conflicts of interest present.