microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2020-2-86-90microbe-1329Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESВирулентность HtpG+ и HtpG− штаммов Yersinia pestis для мышей и морских свинок.Virulence of HtpG+ and HtpG− strains of Yersinia pestis for Mice and Guinea Pigshttps://orcid.org/0000-0001-5472-8724КрасильниковаЕ. А.Krasil’nikovaE. A.

Красильникова Екатерина Александровна

142279, Московская обл., п. Оболенск

Ekaterina A. Krasil’nikova

Obolensk, Moscow Region, 142279

ek.al.krasilnikova@gmail.comhttps://orcid.org/0000-0002-6985-6822ШайхутдиноваР. З.ShaikhutdinovaR. Z.info@obolensk.orghttps://orcid.org/0000-0002-3540-1176СветочТ. Э.SvetochT. E.info@obolensk.orghttps://orcid.org/0000-0003-3946-1755ПлатоновМ. Е.PlatonovM. E.info@obolensk.orghttps://orcid.org/0000-0003-1959-1739КомбароваТ. И.KombarovaT. I.info@obolensk.orghttps://orcid.org/0000-0003-1180-2618ИвановС. А.IvanovS. A.info@obolensk.orghttps://orcid.org/0000-0002-1996-8949ДентовскаяС. В.DentovskayaS. V.info@obolensk.orghttps://orcid.org/0000-0002-5499-7999АнисимовА. П.AnisimovA. P.info@obolensk.orgФБУН «Государственный научный центр прикладной микробиологии и биотехнологии»РоссияState Research Center for Applied Microbiology and BiotechnologyRussian Federation202011072020028690Copyright © Красильникова Е.А., Шайхутдинова Р.З., Светоч Т.Э., Платонов М.Е., Комбарова Т.И., Иванов С.А., Дентовская С.В., Анисимов А.П., 20202020Красильникова Е.А., Шайхутдинова Р.З., Светоч Т.Э., Платонов М.Е., Комбарова Т.И., Иванов С.А., Дентовская С.В., Анисимов А.П.Krasil’nikova E.A., Shaikhutdinova R.Z., Svetoch T.E., Platonov M.E., Kombarova T.I., Ivanov S.A., Dentovskaya S.V., Anisimov A.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/1329

HtpG (высокотемпературный белок G – high-temperature protein G) – бактериальный гомолог высококонсервативного молекулярного шаперона Hsp90 эукариот, играющего важную роль в защите от стресса у многих бактерий. Роль продукта гена htpG, кодирующего синтез высокотемпературного белка G прокариот, в патогенезе бактериальных инфекций до настоящего времени остается не ясной.

Цель работы – изучить функциональную важность HtpG в патогенезе чумы.

Материалы и методы

Материалы и методы. Аминокислотную последовательность HtpG анализировали с использованием программы BLAST. С помощью сайт-направленного мутагенеза гена htpG (YPO3119) получили изогенные наборы на основе аттенуированного и вирулентных штаммов Yersinia pestis. Свойства полученных мутантов оценивали с использованием микробиологических и биологических методик.

HtpG (high-temperature protein G) is a bacterial homologue of the highly conserved molecular chaperone Hsp90 of eukaryotes, which plays an important role in protection against stress in many bacterial species. The role of the htpG gene encoding the synthesis of high-temperature prokaryotic G protein in the pathogenesis of bacterial infections is still unclear.

The aim of this work is to study the functional importance of HtpG in the pathogenesis of plague.

Materials and methods

Materials and methods. Isogenic Yersinia pestis sets based on attenuated and virulent strains differing in the presence of the functional htpG gene (YPO3119) were generated with the help of site-directed mutagenesis. The HtpG amino acid sequence was analyzed using the BLAST program. The properties of the resulting mutant strains were evaluated using microbiological and biological methods.

Results and discussion

Results and discussion. The bioinformatics analysis showed high conservativeness of the HtpG protein within the Y. pestis species (100% identity), as well as 99 % identity with the Y. pseudotuberculosis protein and 96 % identity – Y. enterocolitica protein. Y. pestis htpG knock-out mutants showed increase of susceptibility to temperature and oxidative stress like mutants of the other bacterial species. However, the mutant was not sensitive to osmotic stress and human serum complement. The loss of the ability to synthesize HtpG by plague microbe did not affect the virulence and average life duration of mice and guinea pigs challenged subcutaneously. It means that htpG gene is not a good molecular target for the treatment and/or immunoprophylaxis of plague.

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The authors declare that there are no conflicts of interest present.