microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2014-2-69-72microbe-142Research ArticleМИКРОБИОЛОГИЯMICROBIOLOGYСравнительный анализ способности к ферментации углеводов у типичных штаммов и геновариантов Vibrio cholerae биовара Эль ТорComparative Analysis of the Ability to Ferment Carbohydrates in Typical Strains and Genovariants of Vibrio cholerae Biovar El TorЗадноваС. П.ZadnovaS. P.rusrapi@microbe.ruКрепостноваИ. М.KrepostnovaI. M.rusrapi@microbe.ruЛозовскийЮ. В.LozovskyYu. V.rusrapi@microbe.ruРоссийский научно-исследовательский противочумный институт «Микроб»РоссияRussian Research Anti-Plague Institute “Microbe”Russian Federation201420062014026972Copyright © Заднова С.П., Крепостнова И.М., Лозовский Ю.В., 20142014Заднова С.П., Крепостнова И.М., Лозовский Ю.В.Zadnova S.P., Krepostnova I.M., Lozovsky Y.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/142

Проведено сравнительное изучение способности 6 типичных штаммов и 10 геновариантов V. cholerae биовара Эль Тор, завезенных на территорию России, ферментировать различные углеводы. Выявлено, что геноварианты, как и типичные Эль Тор вибрионы, ферментируют маннозу, сахарозу, манит, но не активны в отношении к арабинозе и рамнозе. Однако показано, что у геновариантов по сравнению с типичными штаммами Эль Тор вибрионов снижена способность к ферментации глюкозы. Геноварианты, как и классические вибрионы, не растут на минимальной среде с 1 % содержанием глюкозы и не способны полностью ферментировать глюкозу до ацетилметилкарбинола в реакции Фогес-Проскауэра. Выдвигается гипотеза, что изменение метаболизма глюкозы в изученных штаммах геновариантов может быть связано с изменением механизма регуляции некоторых генов вирулентности.

Carried out is a comparative analysis of the ability to ferment various carbohydrates in the 6 typical strains and 10 genovariants of V. cholerae biovar El Tor, imported to the territory of the Russian Federation. It has been revealed that genovariants, as well as typical El Tor vibrios, ferment mannose, saccharose, and mannite, but are inactive against arabinose and rhamnose. However, it is demonstrated that genovariants, as compared to typical strains of El Tor vibrios, possess lowered capacity to ferment glucose. Both, genovariants and classical vibrios, do not grow on the minimal media with 1 % content of glucose, and are unable to fully ferment glucose up to acetylmethylcarbinol in Voges-Proskauer reaction test. Put forward is a hypothesis that alteration of glucose metabolism in the studied strains of genovariants is probably due to changes in regulating mechanism of some virulence genes.

холерный вибрионгенетически измененные варианты Эль Тор вибрионовметаболизм глюкозыcholera vibriogenetically altered variants of El Tor vibriosglucose metabolismReferencesЛабораторная диагностика холеры. МУК 4.2.2218-07. М.: Федеральный центр гигиены и эпидемиологии Роспотребнадзора; 2007.[Laboratory diagnostics of cholera. Methodological recommendations]. MR 4.2.2218–07. M.: Rospotrebnadzor Federal Center of Hygiene and Epidemiology; 2007.Смирнова Н.И., Горяев А.А., Заднова С.П., Краснов Я.М., Лозовский Ю.В., Кутырев В.В. Генетическая характеристика клинических штаммов Vibrio cholerae, завезенных на территорию Российской Федерации в современный период. Мол. генет., микробиол. и вирусол. 2011; 3:3-10.Smirnova N.I., Goryaev A.A., Zadnova S.P., Krasnov Ya.M., Lozovsky Yu.V., Kutyrev V.V. [Genetic characteristics of clinical Vibrio cholerae strains imported to the territory of the Russian Federation in the modern period]. Mol. Gen. Mikrobiol. Virusol. 2011; 3:3–10.Callahan L.T., Richardson S.H. Biochemistry of Vibrio cholerae virulence. III. Nutritional requirements for toxin production and the effects of pH on toxin elaboration in chemically defined media. Infect. Immun. 1973; 7:567-72.Callahan L.T., Richardson S.H. Biochemistry of Vibrio cholerae virulence. III. Nutritional requirements for toxin production and the effects of pH on toxin elaboration in chemically defined media. Infect. Immun. 1973; 7:567–72.De S.N., Chatterjee D.N. Experimental study of mechanism of action of Vibrio cholerae on intestional mucous membrane. J. Path. Bacteriol. 1953; 66:559-62.De S.N., Chatterjee D.N. Experimental study of mechanism of action of Vibrio cholerae on intestional mucous membrane. J. Path. Bacteriol. 1953; 66:559–62.Faruque S.M., Nair G.B. Vibrio cholerae genomics and molecular biology. Caister Academic Press, Norfolk, UK; 2008. 218 p.Faruque S.M., Nair G.B. Vibrio cholerae genomics and molecular biology. Caister Academic Press, Norfolk, UK; 2008. 218 p.Iwanaga M., Yamamoto K., Higa N., Ichinose Y., Nakasone N., Tanabe M. Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor. Microbiol. Immunol. 1986; 30:1075-83.Iwanaga M., Yamamoto K., Higa N., Ichinose Y., Nakasone N., Tanabe M. Culture conditions for stimulating cholera toxin production by Vibrio cholerae O1 El Tor. Microbiol. Immunol. 1986; 30:1075–83.Liang W., Silva A.J., Benitez J.A. The cyclic AMP receptor protein modulates colonial morphology in Vibrio cholerae. Appl. Environ. Microbiol. 2007; 73:7482-7.Liang W., Silva A.J., Benitez J.A. The cyclic AMP receptor protein modulates colonial morphology in Vibrio cholerae. Appl. Environ. Microbiol. 2007; 73:7482–7.Kaper J.B., Morris J.G., Levine M.M. Cholera. Clin. Microbiol. Rev. 1995; 8:48-86.Kaper J.B., Morris J.G., Levine M.M. Cholera. Clin. Microbiol. Rev. 1995; 8:48–86.Kovacikova G., Lin W., Skorupski K. Dual regulation of genes involved in acetoin biosynthesis and motility/biofilm formation by the virulence activator AphA and the acetate-responsive LysR-type regulator AlsR in Vibrio cholerae. Mol. Microbiol. 2005; 57:420-33.Kovacikova G., Lin W., Skorupski K. Dual regulation of genes involved in acetoin biosynthesis and motility/biofilm formation by the virulence activator AphA and the acetate-responsive LysR-type regulator AlsR in Vibrio cholerae. Mol. Microbiol. 2005; 57:420–33.Mustachio L.M., Aksit S., Mistry R.H., Scheffler R., Yamada A., Liu J.M. The Vibrio cholerae mannitol transporter is regulated posttranscriptionally by the MtlS small regulatory RNA. J. Bacteriol. 2012; 194:598-606.Mustachio L.M., Aksit S., Mistry R.H., Scheffler R., Yamada A., Liu J.M. The Vibrio cholerae mannitol transporter is regulated posttranscriptionally by the MtlS small regulatory RNA. J. Bacteriol. 2012; 194:598–606.Nair G.B., Faruque S.M., Bhuiyan N.A., Kamruzzaman M., Siddique A.K., Sack D.A. New variants of Vibrio cholerae O1 biotype EL Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J. Clin. Microbiol. 2002; 40:3296-9.Nair G.B., Faruque S.M., Bhuiyan N.A., Kamruzzaman M., Siddique A.K., Sack D.A. New variants of Vibrio cholerae O1 biotype EL Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J. Clin. Microbiol. 2002; 40:3296–9.Poncet S., Milohanic E., Maze A., Nait Abdallah J., Ake F., Larribe M., Deghmane A.E., Taha M.K., Dozot M., De Bolle X., Letesson J.J., Deutscher J. Correlations between carbon metabolism and virulence in bacteria. Infect. Immun. 2009; 16:88-102.Poncet S., Milohanic E., Maze A., Nait Abdallah J., Ake F., Larribe M., Deghmane A.E., Taha M.K., Dozot M., De Bolle X., Letesson J.J., Deutscher J. Correlations between carbon metabolism and virulence in bacteria. Infect. Immun. 2009; 16:88–102.Safa A., Nair G.B., Kong R.Y. Evolution of new variants of Vibrio cholerae O1. Trends Microbiol. 2010; 18(1):46-54.Safa A., Nair G.B., Kong R.Y. Evolution of new variants of Vibrio cholerae O1. Trends Microbiol. 2010; 18(1):46–54.Son M.S., Megli C.J., Kovacikova G., Qadri F., Taylor R.K. Characterization of Vibrio cholerae O1 El Tor biotype variant clinical isolates from Bangladesh and Haiti, including a molecular genetic analysis of virulence genes. J. Clin. Microbiol. 2011; 49:3739-49.Son M.S., Megli C.J., Kovacikova G., Qadri F., Taylor R.K. Characterization of Vibrio cholerae O1 El Tor biotype variant clinical isolates from Bangladesh and Haiti, including a molecular genetic analysis of virulence genes. J. Clin. Microbiol. 2011; 49:3739–49.Yoon S.S., Mekalanos J.J. 2,3-butanediol synthesis and emergence of the Vibrio cholerae El Tor biotype. Infect. Immun. 2006; 74:6547-56.Yoon S.S., Mekalanos J.J. 2,3-butanediol synthesis and emergence of the Vibrio cholerae El Tor biotype. Infect. Immun. 2006; 74:6547–56.

The authors declare that there are no conflicts of interest present.