microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2013-1-81-84microbe-17Research ArticleДИАГНОСТИКАDIAGNOSTICSМультиплексная ПЦР-тест система для обнаружения возбудителя сибирской язвы с детекцией результатов в формате «реального времени»Multiplex Real Time PCR Test-System for the Detection of Anthrax Causative AgentЦыганковаЕ. А.TsygankovaE. A.snipchi@mail.stv.ruЕременкоЕ. И.EremenkoE. I.snipchi@mail.stv.ruРязановаА. Г.RyazanovaA. G.snipchi@mail.stv.ruЦыганковаО. И.TsygankovaO. I.snipchi@mail.stv.ruКуличенкоА. Н.KulichenkoA. N.snipchi@mail.stv.ruСтавропольский научно-исследовательский противочумный институтРоссияStavropol Research Anti-Plague InstituteRussian Federation201320032013018184Copyright © Цыганкова Е.А., Еременко Е.И., Рязанова А.Г., Цыганкова О.И., Куличенко А.Н., 20132013Цыганкова Е.А., Еременко Е.И., Рязанова А.Г., Цыганкова О.И., Куличенко А.Н.Tsygankova E.A., Eremenko E.I., Ryazanova A.G., Tsygankova O.I., Kulichenko A.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/17

Разработана мультиплексная ПЦР-РТ тест-система для обнаружения возбудителя сибирской язвы с гибридизационно-флуоресцентной детекцией в режиме «реального времени» « Bacillus anthracis multiplex 3 FRT». Тест-система содержит внутренний контрольный образец для контроля этапа выделения ДНК, праймеры и зонды к специфическим мишеням ДНК двух плазмидных и хромосомного гена B. anthracis. Это позволяет идентифицировать ди-, моно- и бесплазмидные штаммы B. anthracis, определяя их генетический потенциал патогенности, дифференцировать штаммы возбудителя сибирской язвы и близкородственных сапрофитов рода Bacillus.

Designed is a multiplex PCR-RT test-system for the detection of anthrax causative agent using hybridization fluorescence detection – “ Bacillus anthracis multiplex 3 FRT”. This test-system contains enclosed reference sample for carrying out control procedure in the phase of DNA purification, primers and probes to specific DNA target sequences of the two plasmid and one chromosomal B. anthracis genes. It allows for identification B. anthracis strains both possessing plasmids (one or two) and plasmidless, revealing their genetic pathogenicity potential, and for differentiation between anthrax agent strains and closely related saprophytes belonging to Bacillus species.

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The authors declare that there are no conflicts of interest present.