microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2023-2-140-145microbe-1832Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESПервый случай ящура O/ME-SA/IND-2001 в КазахстанеThe First Case of Foot-and-Mouth Disease Caused by O/ME-SA/IND-2001 Virus in Kazakhstanhttps://orcid.org/0000-0002-6593-0271НахановА. К.NakhanovA. K.

080409, Жамбылская область, Кордайский район, пгт. Гвардейский, ул. Б. Момышулы, 15

15, B. Momyshuly St., uts Gvardeisky, Kordaysky District, Zhambyl Region, 080409

aziz_nk@mail.ruhttps://orcid.org/0000-0002-8123-9210АбеуовХ. Б.AbeuovKh. B.

080409, Жамбылская область, Кордайский район, пгт. Гвардейский, ул. Б. Момышулы, 15

15, B. Momyshuly St., uts Gvardeisky, Kordaysky District, Zhambyl Region, 080409

https://orcid.org/0000-0003-4215-2638ОмароваЗ. Д.OmarovaZ. D.

080409, Жамбылская область, Кордайский район, пгт. Гвардейский, ул. Б. Момышулы, 15

15, B. Momyshuly St., uts Gvardeisky, Kordaysky District, Zhambyl Region, 080409

https://orcid.org/0000-0002-1597-7784ЕрмекбайТ. Т.ErmekbaiT. T.

080409, Жамбылская область, Кордайский район, пгт. Гвардейский, ул. Б. Момышулы, 15

15, B. Momyshuly St., uts Gvardeisky, Kordaysky District, Zhambyl Region, 080409

https://orcid.org/0000-0002-4701-1992БурашевЕ. Д.BurashevE. D.

080409, Жамбылская область, Кордайский район, пгт. Гвардейский, ул. Б. Момышулы, 15

15, B. Momyshuly St., uts Gvardeisky, Kordaysky District, Zhambyl Region, 080409

https://orcid.org/0000-0002-5882-4964ОрынбаевМ. Б.OrynbayevM. B.

080409, Жамбылская область, Кордайский район, пгт. Гвардейский, ул. Б. Момышулы, 15

15, B. Momyshuly St., uts Gvardeisky, Kordaysky District, Zhambyl Region, 080409

РГП на ПХВ «Научно-исследовательский институт проблем биологической безопасности» Министерства здравоохранения Республики КазахстанКазахстанResearch Institute for Biological Safety ProblemsKazakhstan20231407202302140145Copyright © Наханов А.К., Абеуов Х.Б., Омарова З.Д., Ермекбай Т.Т., Бурашев Е.Д., Орынбаев М.Б., 20232023Наханов А.К., Абеуов Х.Б., Омарова З.Д., Ермекбай Т.Т., Бурашев Е.Д., Орынбаев М.Б.Nakhanov A.K., Abeuov K.B., Omarova Z.D., Ermekbai T.T., Burashev E.D., Orynbayev M.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/1832

В Казахстане вспышки ящура регистрировались в 2011–2013 гг. в южных и восточных областях, в которых проводится вакцинация от ящура. В январе 2022 г. в с. Киикты Шетского района Карагандинской области Республики Казахстан отмечены случаи заболевания крупного рогатого скота (КРС), клинически сходные с ящуром. Целью исследования явилось установление причины возникновения болезни и типирование возбудителя. Материалы и методы. Пробы от больных животных анализировали в ПЦР, секвенировали и типировали согласно протоколу, разработанному Международным эпизоотическим бюро. Результаты и обсуждение. В культуре клеток ВНК-21 выделен цитопатогенный агент, идентифицированный методом ОТ-ПЦР-РВ и электронномикроскопически как вирус ящура. С целью определения происхождения вирусов проведено секвенирование и анализ нуклеотидной последовательности. В результате проведенных молекулярно-генетических исследований установлено, что заболевание КРС в Карагандинской области вызвано вирусом ящура, циркулирующим на территории приграничных с Казахстаном стран (Китай, Россия, Монголия) и относящимся к типу О, топотипу ME-SA генетической линии Ind-2001. Выделенный на территории Казахстана вирус ящура генетически связан с линией ящура из Азии и является новым для Казахстана генотипом, отнесенным к топотипу ME-SA генетической линии Ind-2001. Настоящее исследование еще раз доказывает необходимость постоянного генетического типирования вирусов ящура для повышения эффективности профилактических мероприятий.

In Kazakhstan, foot-and-mouth disease (FMD) outbreaks were recorded in 2011–2013 in the southern and eastern regions where FMD vaccination is in place. In January 2022, in Kiikty village of the Shetsky district, Karaganda Region, Republic of Kazakhstan, cases among cattle clinically similar to foot-and-mouth disease were reported. The aim of the research was to establish the cause of the disease and to perform the typing of the pathogen. Materials and methods. Samples from sick animals were tested using PCR, sequenced and typed in compliance with the protocol developed by the International Office for Epizootic Diseases (OIE). Results and discussion. A cytopathogenic agent has been isolated from the BHK-21 cell culture, identified through real-time RT-PCR and electron microscopically as FMD virus. Molecular genetic studies have revealed that the infection of cattle in the Karaganda Region was caused by the FMD virus circulating in the territory of the countries bordering Kazakhstan (China, Russia, Mongolia) and belonging to type O, topotype ME-SA, genetic line Ind-2001. FMD virus isolated in Kazakhstan is genetically related to the FMD line from Asia and is a new FMDV genotype for Kazakhstan, assigned to the ME-SA topotype of the Ind-2001 genetic line. This study once again proves the need for continuous genetic typing of FMD viruses to improve the effectiveness of preventive measures.

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The authors declare that there are no conflicts of interest present.