microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2014-4-56-60microbe-186Research ArticleМИКРОБИОЛОГИЯ, ДИАГНОСТИКАMICROBIOLOGY, DIAGNOSTICSДиагностика ближневосточного респираторного синдрома человекаDiagnostics of Human Middle-East Respiratory SyndromeСтовбаЛ. Ф.StovbaL. F.noemail@neicon.ruЛебедевВ. Н.LebedevV. N.noemail@neicon.ruПетровА. А.PetrovA. A.noemail@neicon.ruКулишВ. С.KulishV. S.noemail@neicon.ruБорисевичС. В.BorisevichS. V.noemail@neicon.ru48 Центральный научно-исследовательский институт Министерства обороны Российской ФедерацииРоссияThe 48th Central Research Institute of the Ministry of DefenseRussian Federation201420122014045660Copyright © Стовба Л.Ф., Лебедев В.Н., Петров А.А., Кулиш В.С., Борисевич С.В., 20142014Стовба Л.Ф., Лебедев В.Н., Петров А.А., Кулиш В.С., Борисевич С.В.Stovba L.F., Lebedev V.N., Petrov A.A., Kulish V.S., Borisevich S.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/186

Ближневосточный респираторный синдром представляет опасное заболевание человека, вызываемое новым коронавирусом. В декабре 2012 г. ВОЗ опубликовала временные руководящие документы по диагностике этого вируса. ВОЗ рекомендует использовать два способа диагностики этого заболевания - двухэтапную обратно-транскриптазную ПЦР в реальном времени (ОТ-ПЦР-РВ) и иммуноферментный анализ. Первый этап ПЦР-диагностики должен включать ОТ-ПЦР-РВ с мишенью на фрагмент генома выше upE гена. Второй (подтверждающий) ПЦР-тест может быть направлен на альтернативные мишени в пределах вирусного генома, не перекрывающиеся с upE геном, и последующее секвенирование, по крайней мере, сегмента одного из вирусных генов и сравнение полученной последовательности с таковыми, депонированными в GenBank. Метод ИФА применяется ретроспективно с целью определения наличия вирусоспецифических антител в сыворотках крови реконвалесцентов. В статье рассмотрены основные характеристики данных методов выявления возбудителя заболевания или специфических антител к нему, и методические рекомендации ВОЗ при диагностике Ближневосточного респираторного синдрома.

Middle-East respiratory syndrome is a human disease caused by a new coronavirus. In December, 2012 WHO published draft regulatory document on diagnostics of the virus. It was recommended to use two methods of disease diagnostics - two-phase reverse-transcription real-time PCR and enzyme immunoassay. The first phase of the PCR-diagnostics should include reverse-transcription real-time PCR targeted on the genome fragment upwards of upE. The second (control) PCR-test may be alternatively targeted within the bonds of the genome, its target being non-crisscross with upE gene. It should include sequencing of, at least, a segment of one of the viral genomes and comparative analysis of the obtained sequence along with the like ones deposited in the GenBank. Enzyme immunoassay is retrospectively used for virus-specific antibody detection in convalescents’ blood sera. Examined are the key specifications of the methods for the detection of ethiological agent or specific antibodies to it, and WHO methodological recommendations in case of Middle-East respiratory syndrome diagnostics.

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The authors declare that there are no conflicts of interest present.