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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">microbe</journal-id><journal-title-group><journal-title xml:lang="ru">Проблемы особо опасных инфекций</journal-title><trans-title-group xml:lang="en"><trans-title>Problems of Particularly Dangerous Infections</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0370-1069</issn><issn pub-type="epub">2658-719X</issn><publisher><publisher-name>Russian Research Anti-Plague Institute “Microbe”</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21055/0370-1069-2024-2-188-192</article-id><article-id custom-type="elpub" pub-id-type="custom">microbe-2013</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>КРАТКИЕ СООБЩЕНИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>BRIEF COMMUNICATIONS</subject></subj-group></article-categories><title-group><article-title>Исследование малярийной паразитемии среди бессимптомных лиц в ряде округов провинций Центральная и Мучинга Замбии – перекрестное исследование</article-title><trans-title-group xml:lang="en"><trans-title>Investigation of Malaria Parasitemia among Asymptomatic Individuals in Designated Districts of Central and Muchinga Provinces of Zambia – A Cross Sectional Study</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8942-8908</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Loti</surname><given-names>P.</given-names></name><name name-style="western" xml:lang="en"><surname>Loti</surname><given-names>P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Patrick Loti, Лусака, 37178,</p><p>10063, Чингола</p></bio><bio xml:lang="en"><p>Patrick Loti, Lusaka;</p><p>Chingola</p></bio><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0374-457X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Nyirenda</surname><given-names>S. S.</given-names></name><name name-style="western" xml:lang="en"><surname>Nyirenda</surname><given-names>S. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Stanley S. Nyirenda, Лусака;</p><p>Балморал, Лусака</p></bio><bio xml:lang="en"><p>Stanley S. Nyirenda, Lusaka;</p><p>Balmoral, Lusaka</p></bio><email xlink:type="simple">stanleynyirenda@yahoo.co.uk</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0002-8656-7552</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Chisulo</surname><given-names>F. K.</given-names></name><name name-style="western" xml:lang="en"><surname>Chisulo</surname><given-names>F. K.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Frank K. Chisulo, </p><p>Мпика</p></bio><bio xml:lang="en"><p>Frank K. Chisulo,</p><p>Mpika</p></bio><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0001-3505-7803</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Chama</surname><given-names>A.</given-names></name><name name-style="western" xml:lang="en"><surname>Chama</surname><given-names>A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Agness Chama, </p><p>Чингола</p></bio><bio xml:lang="en"><p>Agness Chama,</p><p>Chingola</p></bio><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0000-4928-8963</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Singogo</surname><given-names>M.</given-names></name><name name-style="western" xml:lang="en"><surname>Singogo</surname><given-names>M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Majory Singogo,</p><p>Лусака</p></bio><bio xml:lang="en"><p>Majory Singogo,</p><p>Lusaka</p></bio><xref ref-type="aff" rid="aff-5"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Chileshe</surname><given-names>D. B.</given-names></name><name name-style="western" xml:lang="en"><surname>Chileshe</surname><given-names>D. B.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Daniel B. Chileshe,</p><p>Чама</p></bio><bio xml:lang="en"><p>Daniel B. Chileshe,</p><p>Chama</p></bio><xref ref-type="aff" rid="aff-6"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Mwansa</surname><given-names>J. L.</given-names></name><name name-style="western" xml:lang="en"><surname>Mwansa</surname><given-names>J. L.</given-names></name></name-alternatives><bio xml:lang="ru"><p>James L. Mwansa,</p><p>Лусака</p></bio><bio xml:lang="en"><p>James L. Mwansa,</p><p>Lusaka</p></bio><xref ref-type="aff" rid="aff-7"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Университет Чресо, факультет исследований и последипломного образования;&#13;
Северная больница общего профиля Нчанга, клиническая лаборатория</institution><country>Замбия</country></aff><aff xml:lang="en"><institution>Chreso University, Department of Research and Postgraduate Studies;&#13;
Nchanga North General Hospital, Clinical Laboratory</institution><country>Zambia</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Университет Чресо, факультет исследований и последипломного образования;&#13;
Центральный институт ветеринарных исследований</institution><country>Замбия</country></aff><aff xml:lang="en"><institution>Chreso University, Department of Research and Postgraduate Studies;&#13;
Central Veterinary Research Institute</institution><country>Zambia</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Больница общего профиля миссии Чилонга</institution><country>Замбия</country></aff><aff xml:lang="en"><institution>Chilonga Mission General Hospital</institution><country>Zambia</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>MedNest Healthcare and Diagnostics</institution><country>Замбия</country></aff><aff xml:lang="en"><institution>MedNest Healthcare and Diagnostics</institution><country>Zambia</country></aff></aff-alternatives><aff-alternatives id="aff-5"><aff xml:lang="ru"><institution>Больница матери и новорожденного</institution><country>Замбия</country></aff><aff xml:lang="en"><institution>Women and Newborn Hospital</institution><country>Zambia</country></aff></aff-alternatives><aff-alternatives id="aff-6"><aff xml:lang="ru"><institution>Районная больница Чама</institution><country>Замбия</country></aff><aff xml:lang="en"><institution>Chama District Hospital</institution><country>Zambia</country></aff></aff-alternatives><aff-alternatives id="aff-7"><aff xml:lang="ru"><institution>Медицинский университет Апекс, факультет исследований и последипломного образования</institution><country>Замбия</country></aff><aff xml:lang="en"><institution>Lusaka Apex Medical University, Department of Research and Postgraduate Studies</institution><country>Zambia</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2024</year></pub-date><pub-date pub-type="epub"><day>03</day><month>07</month><year>2024</year></pub-date><volume>0</volume><issue>2</issue><fpage>188</fpage><lpage>192</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Loti P., Nyirenda S.S., Chisulo F.K., Chama A., Singogo M., Chileshe D.B., Mwansa J.L., 2024</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="ru">Loti P., Nyirenda S.S., Chisulo F.K., Chama A., Singogo M., Chileshe D.B., Mwansa J.L.</copyright-holder><copyright-holder xml:lang="en">Loti P., Nyirenda S.S., Chisulo F.K., Chama A., Singogo M., Chileshe D.B., Mwansa J.L.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://journal.microbe.ru/jour/article/view/2013">https://journal.microbe.ru/jour/article/view/2013</self-uri><abstract><p>Цель исследования – изучение бессимптомной малярии среди населения, заботящегося о своем здоровье, в четырех районах Замбии. Бессимптомная малярия означает наличие малярийных паразитов in vivo без симптомов и является резервуаром для передачи заболевания.</p><sec><title>Материалы и методы</title><p>Материалы и методы. Кровь, собранную в пробирки с ЭДТА, тестировали с помощью ИХА (наборы для одноэтапного экспресс-тестирования на антиген P.f. [HRP-II] SD Bioline [Южная Корея]), а окрашенные по Гимза тонкие и толстые мазки крови подвергались микроскопическому исследованию.</p></sec><sec><title>Результаты и обсуждение</title><p>Результаты и обсуждение. Из 385 обследованных лиц 84 (21,8 %) дали положительный результат на малярию с помощью ИХА и 101 (26,2 %) – с помощью метода микроскопии. При микроскопическом исследовании у 27 (7,0 %) идентифицированы гаметоциты и у 74 (19,2 %) – трофозоиты. Показали отрицательные результаты при ИХА 17 (4,4 %) образцов, но при исследовании мазка обнаружили трофозоиты и гаметоциты. Анализ по районам показал, что в районе Канчибия наблюдается самый высокий показатель положительных результатов по малярии: 32 (8,3 %) выявлены с помощью экспресс-теста и 35 (8,5 %) – с помощью микроскопии. В провинции Читамбо показатели ИХА и микроскопии были 25 (6,5 %) и 33 (8,4 %) соответственно, в то время как результаты ИХА в округе Мпика и Серенье были 13 (3,4 %) и 14 (3,6 %), а при использовании микроскопии – 12 (3,1 %) в обоих округах [x2 =16,3, p-значение=0,0118]. Продемонстрировали знания и серьезное отношение к малярии 365 из 385 (95 %) участников. Наши результаты подтверждают возможность бессимптомного течения болезни, вызванной возбудителями малярии, включая трофозоиты и гаметоциты, среди внешне здоровых людей, которые представляют риск для здоровья общества. Поэтому крайне важно проводить профилактическую химиотерапию и активизировать усилия по борьбе с переносчиками инфекции, чтобы снизить уровень инфицирования.</p></sec></abstract><trans-abstract xml:lang="en"><p>The aim of the study was to investigate asymptomatic malaria in health-conscious population across four selected districts. Malaria is a life-threatening disease caused by Plasmodium spp. transmitted through bites of infected female Anopheles mosquitoes. Asymptomatic malaria refers to the presence of malaria parasites in vivo without symptoms, which usually provides a reservoir for the disease transmission.</p><sec><title>Materials and methods</title><p>Materials and methods. Blood collected in EDTA underwent testing through RDT (SD Bioline one-step malaria antigen P.f. (HRP-II) rapid test kits), while thin and thick blood smears Giemsa stained were microscopically examined.</p></sec><sec><title>Results and discussion</title><p>Results and discussion. Out of 385 individuals examined, 84 people (21.8 %) tested positive for malaria using RDT and 101/385 (26.2 %) – through microscopy. Microscopic examination further identified 27 individuals (7.0 %) with gametocytes and 74 (19.2 %) – with trophozoites. Intriguingly, 17 (4.4 %) samples showed negative results in RDT but exhibited trophozoites and gametocytes upon smear examination. District-wise analysis demonstrated the highest malaria positivity rate in Kanchibiya district, with 32 cases (8.3 %) detected by RDT and 35 (8.5 %) – through microscopy. Chitambo district followed closely: with RDT and microscopy values of 25 (6.5 %) and 33 (8.4 %), respectively; while Mpika and Serenje districts had 13 (3.4 %) and 14 (3.6 %) prevalence, respectively, with RDT and microscopy at 12 (3.1 %) in both districts [x2 =16.3, p-value=0.0118]. The study also revealed that 365/385 (95 %) of the participants demonstrated knowledge and positive attitudes toward malaria. Our findings accentuate the presence of asymptomatic malaria, encompassing trophozoites and gametocytes, among seemingly healthy individuals which poses a health risk to the community. Therefore, it is imperative to implement preventive chemotherapy and strengthen vector control efforts against malaria in order to reduce the infection rate.</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>малярия</kwd><kwd>бессимптомное течение</kwd><kwd>малярийная паразитемия</kwd><kwd>виды Plasmodium</kwd><kwd>распространенность</kwd></kwd-group><kwd-group xml:lang="en"><kwd>asymptomatic malaria</kwd><kwd>malaria parasitemia</kwd><kwd>Plasmodium species</kwd><kwd>prevalence</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Chourasia M.K., Raghavendra K., Bhatt R.M., Swain D.K., Valecha N., Kleinschmidt I. 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