microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2025-2-86-94microbe-2168Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESСравнительная жизнеспособность Francisella tularensis subsp. mediasiatica и subsp. holarctica в условиях in vivo и in vitroComparative Viability of Francisella tularensis subsp. mediasiatica and subsp. holarctica under in vivo and in vitro Conditionshttps://orcid.org/0000-0002-0383-4698ЕвсееваВ. В.EvseevaV. V.

142279, Московская обл., г.о. Серпухов, п. Оболенск, Территория «Квартал А», 24.

24, “Block A” Territory, Obolensk, Serpukhov, Moscow Region, 142279

https://orcid.org/0000-0001-9478-5563ТитареваГ. М.TitarevaG. M.

142279, Московская обл., г.о. Серпухов, п. Оболенск, Территория «Квартал А», 24.

24, “Block A” Territory, Obolensk, Serpukhov, Moscow Region, 142279

https://orcid.org/0000-0002-6897-3613БахтееваИ. В.BakhteevaI. V.

142279, Московская обл., г.о. Серпухов, п. Оболенск, Территория «Квартал А», 24.

24, “Block A” Territory, Obolensk, Serpukhov, Moscow Region, 142279

https://orcid.org/0000-0002-4681-1787КравченкоТ. Б.KravchenkoT. B.

142279, Московская обл., г.о. Серпухов, п. Оболенск, Территория «Квартал А», 24.

24, “Block A” Territory, Obolensk, Serpukhov, Moscow Region, 142279

https://orcid.org/0000-0001-6309-7645БорзиловА. И.BorzilovA. I.

142279, Московская обл., г.о. Серпухов, п. Оболенск, Территория «Квартал А», 24.

24, “Block A” Territory, Obolensk, Serpukhov, Moscow Region, 142279

https://orcid.org/0000-0003-1959-1739КомбароваТ. И.KombarovaT. I.

142279, Московская обл., г.о. Серпухов, п. Оболенск, Территория «Квартал А», 24.

24, “Block A” Territory, Obolensk, Serpukhov, Moscow Region, 142279

https://orcid.org/0000-0001-8318-4156МироноваР. И.MironovaR. I.

142279, Московская обл., г.о. Серпухов, п. Оболенск, Территория «Квартал А», 24.

24, “Block A” Territory, Obolensk, Serpukhov, Moscow Region, 142279

https://orcid.org/0009-0006-9329-2648КрасавинаН. Ю.KrasavinaN. Yu.

649002, Республика Алтай, Горно-Алтайск, ул. Заводская, 2

2 Zavodskaya St., Gorno-Altaisk, Altai Republic, 649002

https://orcid.org/0000-0002-4064-0155БазароваГ. Х.BazarovaG. Kh.

649002, Республика Алтай, Горно-Алтайск, ул. Заводская, 2

2 Zavodskaya St., Gorno-Altaisk, Altai Republic, 649002

https://orcid.org/0000-0002-6005-3783РождественскийЕ. Н.RozhdestvenskyE. N.

649002, Республика Алтай, Горно-Алтайск, ул. Заводская, 2

2 Zavodskaya St., Gorno-Altaisk, Altai Republic, 649002

https://orcid.org/0000-0002-9501-1380ТимофеевВ. С.TimofeevV. S.

142279, Московская обл., г.о. Серпухов, п. Оболенск, Территория «Квартал А», 24.

24, “Block A” Territory, Obolensk, Serpukhov, Moscow Region, 142279

timofeev_vitalii@mail.ruФБУН «Государственный научный центр прикладной микробиологии и биотехнологии»РоссияState Scientific Center of Applied Microbiology and BiotechnologyRussian FederationФКУЗ «Алтайская противочумная станция»РоссияAltai Plague Control StationRussian Federation202504072025028694Copyright © Евсеева В.В., Титарева Г.М., Бахтеева И.В., Кравченко Т.Б., Борзилов А.И., Комбарова Т.И., Миронова Р.И., Красавина Н.Ю., Базарова Г.Х., Рождественский Е.Н., Тимофеев В.С., 20252025Евсеева В.В., Титарева Г.М., Бахтеева И.В., Кравченко Т.Б., Борзилов А.И., Комбарова Т.И., Миронова Р.И., Красавина Н.Ю., Базарова Г.Х., Рождественский Е.Н., Тимофеев В.С.Evseeva V.V., Titareva G.M., Bakhteeva I.V., Kravchenko T.B., Borzilov A.I., Kombarova T.I., Mironova R.I., Krasavina N.Y., Bazarova G.K., Rozhdestvensky E.N., Timofeev V.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/2168

Цель работы – сравнение способности к выживанию вирулентных штаммов эндемичных для России голарктического и среднеазиатского подвидов туляремийного микроба в водной среде и в условиях организма животного. Материалы и методы. Использовали 11 вирулентных штаммов различных подвидов из Государственной коллекции «ГКПМ-Оболенск». Для оценки выживаемости бактериальные клетки исследуемых штаммов в концентрации 5·106 КОЕ/мл инкубировали в течение 5 недель в автоклавированной водопроводной воде. Жизнеспособность бактериальных клеток Francisella tularensis определяли непосредственно перед экспериментом и далее еженедельно методом высева на плотную питательную среду. Для сравнения штаммов подвидов holarctica и mediasiatica по выживаемости in vivo смесь штаммов трижды пассировалась через мышей с подсчетом соотношения колоний каждого штамма в селезенке при каждом пассаже. Дифференциацию колоний проводили, оценивая длину VNTR-локуса Ft-M20 методом ПЦР с последующим гель-электрофорезом. Результаты и обсуждение. Подвид mediasiatica показал достоверно меньшую способность к выживанию в воде по сравнению с подвидом holarctica. В то же время анализ результатов высевов после сочетанного заражения мышей штаммами голарктического и среднеазиатского подвидов свидетельствует, что подвид mediasiatica размножается в организме хозяина более эффективно, чем подвид holarctica.

The aim of the work was to compare the survival ability of virulent strains of tularemia microbe, mediasiatica and holarctica subspecies, endemic to Russia, in the aquatic environment and in the animal organism. Materials and methods. 11 virulent strains of various subspecies deposited in the State collection “SCPM-Obolensk” were used in the work. To assess the survival rate, bacterial cells of the studied strains at a concentration of 5·106 CFU/ml were incubated for 35 days in autoclaved tap water. The viability of Francisella tularensis bacterial cells was determined immediately before the experiment and then weekly by seeding on agar plates. To compare the holarctica and mediasiatica strains by in vivo survival ability during co-infection, the strain mixture was passaged three times through mice, the ratio of colonies of each strain in the spleen being calculated at each passage. Colony differentiation was performed by assessing the length of the Ft-M20 VNTR locus using PCR and subsequent gel electrophoresis. Results and discussion. Subsp. mediasiatica shows significantly lower ability to survive in water compared to subsp. holarctica. At the same time, analysis of the results of seeding after co-infection of mice with strains belonging to the two subspecies indicates that subsp. mediasiatica reproduces in the host organism more effectively than subsp. holarctica.

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The authors declare that there are no conflicts of interest present.