Materials and Methods. An epidemiological and statistical analysis of the data provided by territorial bodies of the Rospotrebnadzor using developed forms was conducted through the departmental information system report.gsen.ru on a weekly basis from April to October in 2023 and 2024.

Results and discussion. In light of the risk of importation and spread of cholera during the period of 2022–2024, and in accordance with the directives of the Rospotrebnadzor, enhanced monitoring for cholera was implemented across the territory of the Russian Federation. Additional sampling points were introduced at temporary accommodation centers, from wastewater before treatment, from transboundary rivers and other water objects, in cities with population over one million, in port cities, at resorts of the Azov and Black Seas, at wastewater discharge sites from infectious disease hospitals (or departments), and in areas where organized groups of labor migrants live and work. The timing of scheduled monitoring studies was changed in certain territories. The training of specialists was strengthened. The paper presents selected indicators of specialist training, the results of testing for cholera for individuals with symptoms of acute intestinal infections, and the results of monitoring studies on cholera in the territory of the Russian Federation in 2023 and 2024, including the detection of cholera vibrios O1 and non-agglutinating (NAG) vibrios depending on the nature of water use at sampling points, which indicate the presence of conditions for the possible spread of the cholera pathogen by water route in the event of infection importation in 75 % of the country’s entities.