microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2026-1-137-144microbe-2293Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESРезультаты выявления генетических маркеров возбудителей инфекционных болезней, передаваемых иксодовыми клещами, на территории отдельных районов Республики КонгоThe Results of Detecting Genetic Markers of Infectious Disease Pathogens Transmitted by Ixodidae Ticks in Certain Regions of the Republic of the Congohttps://orcid.org/0000-0001-6474-3696НайденоваЕ. В.NaidenovaE. V.

Найденова Екатерина Владимировна

410005, Саратов, ул. Университетская, 46

Ekaterina V. Naidenova

46, Universitetskaya St., Saratov, 410005

rusrapi@microbe.ruhttps://orcid.org/0000-0002-4726-309XЗахаровК. С.ZakharovK. S.

410005, Саратов, ул. Университетская, 46

46, Universitetskaya St., Saratov, 410005

rusrapi@microbe.ruhttps://orcid.org/0000-0001-9600-3801СмолинаА. П.SmolinaA. P.

410005, Саратов, ул. Университетская, 46

46, Universitetskaya St., Saratov, 410005

rusrapi@microbe.ruhttps://orcid.org/0000-0001-6446-0274ШпакИ. М.ShpakI. M.

400066, Волгоград, ул. Голубинская, 7

7, Golubinskaya St., Volgograd, 400066

info@vnipchi.rospotrebnadzor.ruDzeret IndoloG.Dzeret IndoloG.

Браззавиль

Brazzaville

Koukouikila-KoussoundaF.Koukouikila-KoussoundaF.

Браззавиль

Brazzaville

MouelletW. S.MouelletW. S.

Браззавиль

Brazzaville

https://orcid.org/0009-0000-7575-653XКатышевС. Д.KatyshevS. D.

410005, Саратов, ул. Университетская, 46

46, Universitetskaya St., Saratov, 410005

rusrapi@microbe.ruhttps://orcid.org/0000-0002-4909-2394КрасновЯ. М.KrasnovYa. M.

410005, Саратов, ул. Университетская, 46

46, Universitetskaya St., Saratov, 410005

rusrapi@microbe.ruhttps://orcid.org/0000-0002-2234-2760ПолунинаТ. А.PoluninaT. A.

410005, Саратов, ул. Университетская, 46

46, Universitetskaya St., Saratov, 410005

https://orcid.org/0000-0002-9270-523XКотоваН. В.KotovaN. V.

410005, Саратов, ул. Университетская, 46

46, Universitetskaya St., Saratov, 410005

Okemba OngagnaЕ.Okemba OngagnaE.

Браззавиль

Brazzaville

Kangoula-Dia-KikoudiF.Kangoula-Dia-KikoudiF.

Браззавиль

Brazzaville

MayengueP. I.MayengueP. I.

Браззавиль

Brazzaville

NiamaF. R.NiamaF. R.

Браззавиль

Brazzaville

https://orcid.org/0000-0003-3788-3452КутыревВ. В.KutyrevV. V.

410005, Саратов, ул. Университетская, 46

46, Universitetskaya St., Saratov, 410005

rusrapi@microbe.ruРоссийский научно-исследовательский противочумный институт «Микроб»РоссияRussian Research Anti-Plague Institute “Microbe”Russian FederationВолгоградский научно-исследовательский противочумный институтРоссияVolgograd Research Anti-Plague InstituteRussian FederationНациональная лаборатория Института общественного здравоохраненияКонгоNational Laboratory of the Institute of Public HealthCongoЦентр оперативного реагирования на чрезвычайные ситуации в области общественного здравоохраненияКонгоPublic Health Emergency Response CenterCongo20263003202601137144Copyright © Найденова Е.В., Захаров К.С., Смолина А.П., Шпак И.М., Dzeret Indolo G., Koukouikila-Koussounda F., Mouellet W.S., Катышев С.Д., Краснов Я.М., Полунина Т.А., Котова Н.В., Okemba Ongagna Е., Kangoula-Dia-Kikoudi F., Mayengue P.I., Niama F.R., Кутырев В.В., 20262026Найденова Е.В., Захаров К.С., Смолина А.П., Шпак И.М., Dzeret Indolo G., Koukouikila-Koussounda F., Mouellet W.S., Катышев С.Д., Краснов Я.М., Полунина Т.А., Котова Н.В., Okemba Ongagna Е., Kangoula-Dia-Kikoudi F., Mayengue P.I., Niama F.R., Кутырев В.В.Naidenova E.V., Zakharov K.S., Smolina A.P., Shpak I.M., Dzeret Indolo G., Koukouikila-Koussounda F., Mouellet W.S., Katyshev S.D., Krasnov Y.M., Polunina T.A., Kotova N.V., Okemba Ongagna E., Kangoula-Dia-Kikoudi F., Mayengue P.I., Niama F.R., Kutyrev V.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/2293

Исследование проводилось с целью выявления генетических маркеров (РНК, 16S рРНК или ДНК) возбудителей природно-очаговых инфекционных болезней бактериальной, вирусной и риккетсиозной природы в пробах иксодовых клещей, собранных на территории отдельных регионов Республики Конго. Материалы и методы. Наличие маркеров возбудителей лихорадки Ку, риккетсиозов, анаплазмозов, эрлихиозов, туляремии, Крымской геморрагической лихорадки, клещевого энцефалита определяли методом полимеразной цепной реакции с использованием диагностических препаратов российского производства. Исследовано 509 пулов эктопаразитов 5 видов, собранных в департаментах Пул, Кювет и окрестностях г. Браззавиль в 2025 г. Результаты и обсуждение. В материале от клещей обнаружены: РНК вируса Крымской-Конго геморрагической лихорадки (ККГЛ) (0,9 % от всех исследуемых проб), кДНК Borrelia burgdorferi s.l. (3,9 %), ДНК Coxiella burnetii (1,8 %) и риккетсии группы клещевых пятнистых лихорадок (45,2 %). Положительные результаты зарегистрированы в 264 пробах из 509 (51,9 %). Генетические маркеры возбудителей клещевого энцефалита, эрлихиоза, анаплазмоза и туляремии не обнаружены. Некоторые образцы, в которых были выявлены маркеры вируса ККГЛ, Rickettsia spp. и Coxiella burnetii в высокой концентрации, подвергали генетическому анализу методом высокопроизводительного секвенирования через нанопоры на платформе MinIon (Oxford Nanopore Technologies, Великобритания), в результате чего доказано их отношение к заявленным таксономическим группам. На основании генетического анализа последовательностей S-сегмента изолятов вируса ККГЛ продемонстрирована их принадлежность к генотипу «Африка-1» со степенью гомологии от 98,8 до 100 %. Полученные сведения показывают необходимость продолжения изучения распространения возбудителей природно-очаговых инфекционных болезней, передаваемых клещами, на территории Республики Конго и организации систематического эпизоотологического мониторинга.

This study was conducted in order to identify genetic markers of pathogens (RNA, 16S rRNA or DNA) of natural-focal infectious diseases of bacterial, viral and rickettsial origin in suspensions of Ixodidae ticks collected in certain regions of the Republic of the Congo. Materials and methods. The presence of markers of Q fever, rickettsiosis, anaplasmosis, ehrlichiosis, tularemia, Crimean hemorrhagic fever, tick-borne encephalitis agents was determined applying polymerase chain reaction using Russian-made diagnostic drugs. A total of 509 combined samples of ectoparasites of 5 species collected in the prefectures of Pool, Cuvette and the vicinity of Brazzaville in 2025 were studied. Results and discussion. As a result of the work, RNA of Crimean-Congo hemorrhagic fever virus (0.9 % of all samples studied), cDNA of Borrelia burgdorferi s.l. (3.9 %), DNA of Coxiella burnetii (1.8 %) and rickettsia of the tick-borne spotted fever group (45.2 %) were detected in the material from ticks. Positive results were recorded in 264 out of 509 samples (51.9 %). No genetic markers of tick-borne encephalitis, ehrlichiosis, anaplasmosis and tularemia agents were found. Some samples in which markers of the Crimean-Congo hemorrhagic fever virus, Rickettsia spp., and Coxiella burnetii in high concentrations had been identified were subjected to genetic analysis using high-throughput sequencing via nanopores on the MinIon platform (Oxford Nanopore Technologies, Great Britain); their taxonomic appurtenance to the stated systematic groups was confirmed. Based on the genetic analysis of the S segment sequences of Crimean-Congo hemorrhagic fever virus isolates, their belonging to the Africa-1 genotype with a degree of homology ranging from 98.8 % to 100 % was demonstrated. The data obtained evidences the need to continue studying the prevalence of naturalfocal infectious diseases transmitted by ticks in the Republic of the Congo and implement systematic epizootiological monitoring.

иксодовые клещиЦентральная АфрикаРеспублика Конгополимеразная цепная реакцияполимеразная цепная реакция с обратной транскрипциейвирус Крымской-Конго геморрагической лихорадкивирус клещевого энцефалитариккетсии группы клещевых пятнистых лихорадокCoxiella burnetiiBorrelia burgdorferi s.l.Francisella tularensisвозбудители эрлихиозавозбудители анаплазмозаIxodidae ticksCentral AfricaRepublic of the Congopolymerase chain reactionreverse transcription polymerase chain reactionCrimean-Congo hemorrhagic fever virustick-borne encephalitis virusrickettsia of the tickborne spotted fever groupCoxiella burnetiiBorrelia burgdorferi s.l.Francisella tularensispathogens of ehrlichiosisand pathogens of anaplasmosisИсследования проводились в рамках выполнения распоряжения Правительства Российской Федерации от 13 июня 2023 г. № 1563-р по оказанию содействия странам Африки в обеспечении санитарно-эпидемиологического благополучия населения, профилактики и борьбы с инфекционными заболеваниями. 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The authors declare that there are no conflicts of interest present.