References

microbe

Проблемы особо опасных инфекций

Problems of Particularly Dangerous Infections

0370-10692658-719X

Russian Research Anti-Plague Institute “Microbe”

10.21055/0370-1069-2015-3-58-64

microbe-247

Research Article

МИКРОБИОЛОГИЯ

MICROBIOLOGY

Моноклональные антитела и рекомбинантные белки для иммунодиагностики болезни, вызванной вирусом Эбола

Monoclonal Antibodies and Recombinant Proteins for Immune-Diagnostics of Ebola Virus Disease

Казачинская

Е. И.

Kazachinskaya

E. I.

vector@vector.nsc.ru

Никонорова

Ю. В.

Nikonorova

Yu. V.

vector@vector.nsc.ru

Локтев

В. Б.

Loktev

V. B.

vector@vector.nsc.ru

Государственный научный центр вирусологии и биотехнологии «Вектор»; Новосибирский государственный университетРоссияState Research Centre of Virology and Biotechnology “Vector”; Novosibirsk State UniversityRussian Federation

Государственный научный центр вирусологии и биотехнологии «Вектор»РоссияState Research Centre of Virology and Biotechnology “Vector”Russian Federation

Государственный научный центр вирусологии и биотехнологии «Вектор»; Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук; Новосибирский государственный университетРоссияState Research Centre of Virology and Biotechnology “Vector”; Institute of Cytology and Genetics of the RAS Siberian Branch; Novosibirsk State UniversityRussian Federation

2015

20092015

035864

2015

Казачинская Е.И., Никонорова Ю.В., Локтев В.Б.

Kazachinskaya E.I., Nikonorova Y.V., Loktev V.B.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://journal.microbe.ru/jour/article/view/247

В обзоре обобщены литературные данные по исследованию вирусных антигенов и антител, специфичных к вирусу Эбола, пригодных для разработки тест-систем для иммунодиагностики вызываемого им заболевания. Представлены результаты работ авторов по получению гибридомных моноклональных антител (МКА), специфичных к структурным вирусным белкам NP, VP40 и VP35, рекомбинантных белков NP, VP40 и VP35 в прокариотической системе E. coli, а также возможность использования панелей МКА для выявления вирусных и рекомбинантных белков ВЭ в иммуноферментном анализе.

The review summarizes the literature data on the studies of viral antigens and antibodies, specific to Ebola virus, suitable for the development of a test-system for immune-diagnostics of the disease caused by them. Represented are the results of investigations on the obtainment of hybridoma monoclonal antibodies (MCA), specific to structural viral proteins NP, VP40, and VP35; recombinant proteins NP, VP40, and VP35 in prokaryotic system of E. coli; as well as the possibility to utilize MCA panels for EV viral and recombinant proteins’ detection in ELISA.

вирус Эболарекомбинантные белкимоноклональные антителаиммунодиагностикаEbola virusrecombinant proteinsmonoclonal antibodiesimmune-diagnostics

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The authors declare that there are no conflicts of interest present.