microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2015-4-55-57microbe-266Research ArticleМИКРОБИОЛОГИЯMICROBIOLOGYТипирование Yersinia pseudotuberculosis с помощью мультилокусного анализа вариабельного числа тандемных повторовYersinia pseudotuberculosis Typing Using Multi-Locus Variable-Number Tandem Repeat AnalysisЕвсееваВ. В.EvseevaV. V.info@obolensk.orgПлатоновМ. Е.PlatonovM. E.info@obolensk.orgДентовскаяС. В.DentovskayaS. V.info@obolensk.orgАнисимовА. П.AnisimovA. P.info@obolensk.orgГосударственный научный центр прикладной микробиологии и биотехнологииРоссияState Research Center for Applied Microbiology and BiotechnologyRussian Federation201520122015045557Copyright © Евсеева В.В., Платонов М.Е., Дентовская С.В., Анисимов А.П., 20152015Евсеева В.В., Платонов М.Е., Дентовская С.В., Анисимов А.П.Evseeva V.V., Platonov M.E., Dentovskaya S.V., Anisimov A.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/266

Проведена оценка эффективности используемого для дифференциации и кластеризации штаммов чумного микроба метода MLVA25 в отношении возбудителя псевдотуберкулеза, а также поиск штаммов Y. pseudotuberculosis наиболее филогенетически близких Yersinia pestis с помощью варианта этого метода с использованием не 25, а 14 VNTR локусов. При сравнительном исследовании 71 изолята Y. pseudotuberculosis и пяти штаммов Y. pestis вариантами метода MLVA25 и MLVA14 выявлено 75 и 54 генотипа соответственно. Штаммам псевдотуберкулезного микроба отдельных сероваров соответствовали определенные MLVA типы. По данным MLVA14, наиболее близкими к чумному микробу из 221 исследованного штамма Y. pseudotuberculosis оказались представители кластеров, включающих изоляты псевдотуберкулезного микроба MLST типов ST19 (серовар O:3) и ST43 (серовар O:1b).

Estimated has been efficacy of MLVA25 method, used for differentiation and clusterization of Yersinia pestis strains, in reference to Yersinia pseudotuberculosis strains, as well as the search of Y. pseudotuberculosis strains mostly closely related to Y. pestis . Applied was the reduced version of the technique deploying 14 out of 25 VNTR loci. Comparative study of 71 Y. pseudotuberculosis isolates and five Y. pestis strains by means of MLVA25 and MLVA14 variants revealed 75 and 54 genotypes, respectively. Y. pseudotuberculosis strains of certain serovars corresponded to certain clusters of MLVA types. According to MLVA14 typing of 221 Y. pseudotuberculosis isolates the clusters including the strains belonging to MLST types ST19 (serovar O:3) and ST43 (serovar O:1b) were the most closely related to Y. pestis .

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The authors declare that there are no conflicts of interest present.