microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2017-2-36-39microbe-388Research ArticleМИКРОБИОЛОГИЯMICROBIOLOGYРОЛЬ МАЛЫХ РНК В КОНТРОЛЕ ЭКСПРЕССИИ ГЕНОВ, ВОВЛЕЧЕННЫХ В РЕАЛИЗАЦИЮ ПАТОГЕННОСТИ VIBRIO CHOLERAЕTHE ROLE OF SMALL RNAS IN CONTROLLING EXPRESSION OF GENES INVOLVED IN THE IMPLEMENTATION OF VIBRIO CHOLERAE PATHOGENICITYПисановР. В.PisanovR. V.

344002, Ростов-на-Дону, ул. М.Горького, 117/40

117/40, M.Gor`kogo St., Rostov-on-Don, 344002

pisanov_rv@antiplague.ruВодопьяновА. С.Vodop’yanovA. S.

344002, Ростов-на-Дону, ул. М.Горького, 117/40

117/40, M.Gor`kogo St., Rostov-on-Don, 344002

plague@aaanet.ruСимаковаД. И.SimakovaD. I.

344002, Ростов-на-Дону, ул. М.Горького, 117/40

117/40, M.Gor`kogo St., Rostov-on-Don, 344002

plague@aaanet.ruРостовский-на- Дону научно-исследовательский противочумный институтРоссияRostov-on-Don Research Anti-Plague InstituteRussian Federation201720062017023639Copyright © Писанов Р.В., Водопьянов А.С., Симакова Д.И., 20172017Писанов Р.В., Водопьянов А.С., Симакова Д.И.Pisanov R.V., Vodop’yanov A.S., Simakova D.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/388

В настоящее время малые РНК бактерий с регуляторными функциями характеризуют как гетерогенную совокупность высокоструктурированных одноцепочечных РНК, обычно не транслирующихся в белки. Большую группу малых РНК составляют малые регуляторные РНК, функционирующие по механизму комплементарного взаимодействия оснований малой регуляторной РНК с матричными РНК бактерий. В обзоре приведено описание механизмов непосредственного участия малых РНК в реализации патогенных свойств, формировании биопленки и регуляции вирулентности штаммов V. cholerae. В частности, описаны Hfq-зависимые малые РНК, управляющие экспрессией генов, ответственных за вирулентность и формирование биопленки V. choleraе; представлено влияние малых РНК на систему секреции шестого типа; показано формирование везикул холерного вибриона, управляемого малой РНК, имеющих важное значение для колонизирующей способности холерных вибрионов. Приводится описание такого способа регуляции на уровне РНК, как «рибосвитчи» (РНК-переключатели). 

Currently, bacterial small RNAs with regulatory functions are characterized as a heterogeneous claster of highly structured single-stranded RNAs which usually would not be translated into proteins. A large group of small RNAs consists of small regulatory RNAs functioning through the mechanism of complementary interaction between small regulatory RNA bases and bacterial messenger RNAs (mRNA). The review provides the description of direct participation of small RNA mechanisms in realization of pathogenic properties, biofilm formation and virulence regulation in V. cholerae strains. In particular, the Hfq-dependent small RNAs that control the expression of genes responsible for virulence and biofilm formation of V. choleraе, and the impact of small RNA on the secretion system of VI type are presented. We also characterized the small RNAs–controlled process of V. cholerae vesicles formation, which have a great significance for colonization ability of cholera vibrios. In addition, such method of regulation at RNA level as “riboswitches”, (RNA switches) is described. 

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The authors declare that there are no conflicts of interest present.