<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">microbe</journal-id><journal-title-group><journal-title xml:lang="ru">Проблемы особо опасных инфекций</journal-title><trans-title-group xml:lang="en"><trans-title>Problems of Particularly Dangerous Infections</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0370-1069</issn><issn pub-type="epub">2658-719X</issn><publisher><publisher-name>Russian Research Anti-Plague Institute “Microbe”</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21055/0370-1069-2010-2(104)-60-65</article-id><article-id custom-type="elpub" pub-id-type="custom">microbe-528</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МИКРОБИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>MICROBIOLOGY</subject></subj-group></article-categories><title-group><article-title>Изучение генетического разнообразия штаммов сибиреязвенного микроба из коллекции ГНЦ ПМБ</article-title><trans-title-group xml:lang="en"><trans-title>Study of the Genetic Diversity of the Anthrax Microbe Strains from the Collection of SRC AMB</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Шишкова</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Shishkova</surname><given-names>N. A.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мокриевич</surname><given-names>А. Н.</given-names></name><name name-style="western" xml:lang="en"><surname>Mokrievich</surname><given-names>A. N.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Платонов</surname><given-names>М. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Platonov</surname><given-names>M. E.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Светоч</surname><given-names>Т. Э.</given-names></name><name name-style="western" xml:lang="en"><surname>Svetoch</surname><given-names>T. E.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Маринин</surname><given-names>Л. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Marinin</surname><given-names>L. I.</given-names></name></name-alternatives><email xlink:type="simple">noemail@neicon.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Государственный научный центр прикладной микробиологии и биотехнологии</institution><country>Россия</country></aff><aff xml:lang="en"><institution>State Research Center for Applied Microbiology and Biotechnology</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2010</year></pub-date><pub-date pub-type="epub"><day>20</day><month>04</month><year>2010</year></pub-date><volume>0</volume><issue>2(104)</issue><fpage>60</fpage><lpage>65</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Шишкова Н.А., Мокриевич А.Н., Платонов М.Е., Светоч Т.Э., Маринин Л.И., 2010</copyright-statement><copyright-year>2010</copyright-year><copyright-holder xml:lang="ru">Шишкова Н.А., Мокриевич А.Н., Платонов М.Е., Светоч Т.Э., Маринин Л.И.</copyright-holder><copyright-holder xml:lang="en">Shishkova N.A., Mokrievich A.N., Platonov M.E., Svetoch T.E., Marinin L.I.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://journal.microbe.ru/jour/article/view/528">https://journal.microbe.ru/jour/article/view/528</self-uri><abstract><p>В работе представлены данные по VNTR - типированию сибиреязвенных штаммов из коллекции ГНЦ ПМБ. Всего проанализировано 25 штаммов Bacillus anthracis, имеющих разное происхождение и территориальную принадлежность. Использование схемы типирования по P.Keim et al. позволило соотнести российские штаммы со штаммами других коллекций. Было обнаружено 4 новых аллели: Dakkar - VrrA (296), 157 - VrrB2 (180), Dakkar - VrrC2 (478) и M-29 - pXO1 (138). Большая часть штаммов относится к группе A (величина VrrB1 фрагмента равна 229 п.о.) и лишь два штамма (1051 и 157) имеют величину VrrB1 фрагмента 256 п.н. Не укладываются ни в один из 89 описанных P.Keim et al. генотипов следующие штаммы из коллекции ГНЦ ПМБ: 1«Коломна», Ч-7, 15, M-29, 34, 81/1, 157, 228, 1051 и Dakkar. Показано генетическое разнообразие сибиреязвенных штаммов, выделенных из разных источников и находящихся в коллекции ГНЦ ПМБ.</p></abstract><trans-abstract xml:lang="en"><p>Data on VNTR typing of anthrax strains from SRC AMB collection are presented. 25 Bacillus anthracis strains of different origin and isolated in different territories have been analyzed. Application of the typing scheme of P.Keim et al. enabled to correlate Russian strains with strains of other collections. 4 new alleles: Dakkar-VrrA(296), 157-VrrB2(180), Dakkar-VrrC2(478) and M-29-pXO1(138) were determined. Major part of the strains refers to the A group (the size of VrrB1 fragment is 229 b.p.) and only two strains (1051 and 157) possess VrrB1 fragment of 229 b.p. The following strains of SRC AMB collection: 1 Kolomna, Ch-7, 15, M-29, 34, 81/1, 157, 228, 1051 and Dakkar do not go in any of 89 genotypes described by P.Keim et al. Genetic diversity of the anthrax strains isolated from different sources and presented in SRC AMB collection is shown.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>B. anthracis</kwd><kwd>типирование</kwd><kwd>штамм</kwd><kwd>геном</kwd></kwd-group><kwd-group xml:lang="en"><kwd>B. anthracis</kwd><kwd>typing</kwd><kwd>strain</kwd><kwd>genome</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Цыганкова Е.А., Еременко Е.И., Цыганкова О.И. Генетическое разнообразие локуса vrrA у штаммов Bacillus anthracis, выделенных на территории стран СНГ. В кн.: Чрезвычайные ситуации международного значения в общественном здравоохранении в решениях Санкт-Петербургского саммита «Группы восьми» и санитарная охрана территорий государств-участников Содружества Независимых Государств. Оболенск; 2006. С. 125-6.</mixed-citation><mixed-citation xml:lang="en">Цыганкова Е.А., Еременко Е.И., Цыганкова О.И. Генетическое разнообразие локуса vrrA у штаммов Bacillus anthracis, выделенных на территории стран СНГ. В кн.: Чрезвычайные ситуации международного значения в общественном здравоохранении в решениях Санкт-Петербургского саммита «Группы восьми» и санитарная охрана территорий государств-участников Содружества Независимых Государств. Оболенск; 2006. С. 125-6.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Andersen G.L., Simchock J.M., Wilson K.H. Identification of a region of genetic variability among Bacillus anthracis strains and related species. J. Bacteriol. 1996; 178(1):377-84.</mixed-citation><mixed-citation xml:lang="en">Andersen G.L., Simchock J.M., Wilson K.H. Identification of a region of genetic variability among Bacillus anthracis strains and related species. J. Bacteriol. 1996; 178(1):377-84.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Jackson P.I., Walters E.A., Kalif A.S., Richmond K.L., Adair D.M., Hill K.K. et al. Сharacterization of the Variable-Namber Tandem Repeats in vrr A from different Bacillus anthracis isolates. Appl. Environ. Microbiol. 1997; 63(4):1400-5.</mixed-citation><mixed-citation xml:lang="en">Jackson P.I., Walters E.A., Kalif A.S., Richmond K.L., Adair D.M., Hill K.K. et al. Сharacterization of the Variable-Namber Tandem Repeats in vrr A from different Bacillus anthracis isolates. Appl. Environ. Microbiol. 1997; 63(4):1400-5.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Keim P., Kalif A., Schupp J., Hill K., Travis S., Richmond K. et al. Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers. J. Bacteriol. 1997; 179(3):818-24.</mixed-citation><mixed-citation xml:lang="en">Keim P., Kalif A., Schupp J., Hill K., Travis S., Richmond K. et al. Molecular evolution and diversity in Bacillus anthracis as detected by amplified fragment length polymorphism markers. J. Bacteriol. 1997; 179(3):818-24.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Keim P., Price L.B., Klevytska A.M., Smith K., Schupp J.M., Okinaka R. et al. Multiple-locus VNTR analysis (MLVA) reveals genetic relationships within Bacillus anthracis. J. Bacteriol. 2000; 182:2928-36.</mixed-citation><mixed-citation xml:lang="en">Keim P., Price L.B., Klevytska A.M., Smith K., Schupp J.M., Okinaka R. et al. Multiple-locus VNTR analysis (MLVA) reveals genetic relationships within Bacillus anthracis. J. Bacteriol. 2000; 182:2928-36.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Jolley K.A. et al. Sequence type analysis and recombinational tests (START). Bioinformatics. 2001; 17:1230-1.</mixed-citation><mixed-citation xml:lang="en">Jolley K.A. et al. Sequence type analysis and recombinational tests (START). Bioinformatics. 2001; 17:1230-1.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
