microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2010-4(106)-28-31microbe-559Research ArticleМИКРОБИОЛОГИЯ, ДИАГНОСТИКАMICROBIOLOGY, DIAGNOSTICSВнутривидовая дифференциация и определение очаговой принадлежности штаммов чумного микроба методом вычитающего рестрикционного фингерпринтингаIntra-Species Differentiation and Determination of the Focal Belonging of Plague Microbe Strains Using Subtracted Restriction FingerprintingГаеваА. В.GaevaA. V.microbe@san.ruБулгаковаЕ. Г.BulgakovaE. G.noemail@neicon.ruКиреевМ. Н.KireevM. N.noemail@neicon.ruАнисимоваЛ. В.AnisimovaL. V.noemail@neicon.ruНовичковаЛ. А.NovichkovaL. A.noemail@neicon.ruКутыревВ. В.KutyrevV. V.noemail@neicon.ruРоссийский научно-исследовательский противочумный институт «Микроб»РоссияRussian Research Anti-Plague Institute MicrobeRussian Federation20102008201004(106)2831Copyright © Гаева А.В., Булгакова Е.Г., Киреев М.Н., Анисимова Л.В., Новичкова Л.А., Кутырев В.В., 20102010Гаева А.В., Булгакова Е.Г., Киреев М.Н., Анисимова Л.В., Новичкова Л.А., Кутырев В.В.Gaeva A.V., Bulgakova E.G., Kireev M.N., Anisimova L.V., Novichkova L.A., Kutyrev V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/559

Проведено генетическое типирование штаммов чумного микроба методом вычитающего рестрикционного фингерпринтинга (ВРФ). Показана возможность внутривидовой дифференциации и определения очаговой принадлежности изученных штаммов чумного микроба. В некоторых очагах циркулируют штаммы с разными ВРФ профилями, что предполагает возможность использования данного метода генетического типирования при расследовании локальных вспышек.

Plague microbe strains were typed using subtracted restriction fingerprinting (SRF). Intra-species differentiation and determination of the focal belonging of the studied plague microbe strains was demonstrated. Strains with different SRF patterns circulate in some foci suggesting the possibility to use this genetic typing method for local outbreaks investigation.

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The authors declare that there are no conflicts of interest present.