microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2011-1(107)-49-52microbe-587Research ArticleМИКРОБИОЛОГИЯMICROBIOLOGYИзмененные варианты возбудителя холеры, выделенные на территории Российской ФедерацииAltered Variants of Cholera Agent Isolated in the Territory of the Russian FederationГоряевА. А.GoryaevA. A.microbe@san.ruЗадноваС. П.ZadnovaS. P.microbe@san.ruШубинаА. В.ShubinaA. V.noemail@neicon.ruКрасновЯ. М.KrasnovYa. M.noemail@neicon.ruСмирноваН. И.SmirnovaN. I.noemail@neicon.ruРоссийский научно-исследовательский противочумный институт «Микроб»РоссияRussian Research Anti-Plague Institute MicrobeRussian Federation20112002201101(107)4952Copyright © Горяев А.А., Заднова С.П., Шубина А.В., Краснов Я.М., Смирнова Н.И., 20112011Горяев А.А., Заднова С.П., Шубина А.В., Краснов Я.М., Смирнова Н.И.Goryaev A.A., Zadnova S.P., Shubina A.V., Krasnov Y.M., Smirnova N.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/587

Проведен ретроспективный генетический анализ штаммов холерного вибриона, вызвавших вспышки и спорадические случаи холеры на территории Российской Федерации с 1993 по 2006 год. Установлено, что уже с 1993 г. заболевание вызывали измененные штаммы Vibrio cholerae биовара эльтор, содержащие в геноме профага СТХφ ген ctxB классического типа, кодирующий В-субъединицу холерного токсина. Кроме того, обнаружено два штамма, у которых в геноме профага присутствовал ген rstRклассического типа.

Carried out was the retrospective genetic analysis of cholera vibrio strains which caused the outbreaks and sporadic cases of cholera in the territory of the Russian Federation from 1993 to 2006. It was elucidated that beginning from 1993 the disease had been caused by the altered strains of V. cholerae El Tor containing ctxB gene of classical type, encoding B subunit of cholera toxin, in their CTXφ prophage genome. In addition, identified were two strains whose prophage genome contained classical type rstR gene.

Vibrio choleraeпрофаг CTXφизмененные вариантыVibrio choleraeCTXφ prophagealtered variantsReferencesСмирнова Н.И., Кутырев В.В. Эволюция генома возбудителя холеры. Мол. генет., микробиол. и вирусол. 2004; 4:3-13.Smirnova N.I., Kutyrev V.V. [Evolution of the cholera agent genome]. Mol. Gen. Mikrobiol. Virusol. 2004; 4:3–13.Bhattacharya T., Chatterjee S., Maiti D., et al. Molecular analysis of the rstR and orfU genes of the CTX prophages integrated in the small chromosomes of environmental Vibrio cholerae non-O1, non-O139 strains. Environ. Microbiol. 2006; 8:526-34.Bhattacharya T., Chatterjee S., Maiti D., et al. Molecular analysis of the rstR and orfU genes of the CTX prophages integrated in the small chromosomes of environmental Vibrio cholerae non-O1, non-O139 strains. Environ. Microbiol. 2006; 8:526-34.Chun J, Grim C.J., Hasan N.A., Lee J.H., Choi S.Y., Haley B.J., et al. Comparative genomics reveals mechanism for short-term and long-term clonal transitions in pandemic Vibrio cholerae. Proc. Natl. Acad. Sci. USA. 2009; 106(36):15442-7.Chun J, Grim C.J., Hasan N.A., Lee J.H., Choi S.Y., Haley B.J., et al. Comparative genomics reveals mechanism for short-term and long-term clonal transitions in pandemic Vibrio cholerae. Proc. Natl. Acad. Sci. USA. 2009; 106(36):15442-7.Faruque S.M., Nair G.B. Molecular ecology of toxigenic Vibrio cholerae. Microbiol. Immunol. 2002; 46:59-66.Faruque S.M., Nair G.B. Molecular ecology of toxigenic Vibrio cholerae. Microbiol. Immunol. 2002; 46:59-66.Fields P.I., Popovic T., Wachsmuth K., Olsvik O. Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J. Clin. Microbiol. 1992; 30(8):2118-21.Fields P.I., Popovic T., Wachsmuth K., Olsvik O. Use of polymerase chain reaction for detection of toxigenic Vibrio cholerae O1 strains from the Latin American cholera epidemic. J. Clin. Microbiol. 1992; 30(8):2118-21.Keasler S.P., Hall R.H. Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction. Lancet. 1993; 341(8861):1661.Keasler S.P., Hall R.H. Detecting and biotyping Vibrio cholerae O1 with multiplex polymerase chain reaction. Lancet. 1993; 341(8861):1661.Kumar P., Jain M., Goel A.K., Bhadauria S., Sharma S.K., Kamboj D.V., et al. A large cholera outbreak due to a new cholera toxin variant of the Vibrio cholerae O1 El Tor biotype in Orissa, Eastern India. J. Med. Microbiol. 2009; 58:234-8.Kumar P., Jain M., Goel A.K., Bhadauria S., Sharma S.K., Kamboj D.V., et al. A large cholera outbreak due to a new cholera toxin variant of the Vibrio cholerae O1 El Tor biotype in Orissa, Eastern India. J. Med. Microbiol. 2009; 58:234-8.Morita M., Ohnishi M., Arakawa E., Bhuiyan N.A., Nusrin S., Alam M., et al. Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor. Microbiol. Immunol. 2008; 52:314-7.Morita M., Ohnishi M., Arakawa E., Bhuiyan N.A., Nusrin S., Alam M., et al. Development and validation of a mismatch amplification mutation PCR assay to monitor the dissemination of an emerging variant of Vibrio cholerae O1 biotype El Tor. Microbiol. Immunol. 2008; 52:314-7.Nair G.B., Faruque S.M., Bhuiyan N.A., Kamruzzaman M., Siddique A.K., Sack D.A. New variants of Vibrio cholerae O1 biotype EL Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J. Clin. Microbiol. 2002; 40:3296-9.Nair G.B., Faruque S.M., Bhuiyan N.A., Kamruzzaman M., Siddique A.K., Sack D.A. New variants of Vibrio cholerae O1 biotype EL Tor with attributes of the classical biotype from hospitalized patients with acute diarrhea in Bangladesh. J. Clin. Microbiol. 2002; 40:3296-9.Safa A., Sultana J., Cam P.D., et al. Classical cholera toxin producing Vibrio cholerae O1 hybrid El Tor strains in Asia and Africa. Emerg. Infect. Dis. 2008; 14:987-8.Safa A., Sultana J., Cam P.D., et al. Classical cholera toxin producing Vibrio cholerae O1 hybrid El Tor strains in Asia and Africa. Emerg. Infect. Dis. 2008; 14:987-8.Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24(8):1596-9.Tamura K., Dudley J., Nei M., Kumar S. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol. 2007, 24(8):1596-9.Waldor M.K., Rubin E.J., Gregory D.N. et al. Regulation, replication, and integration functions of the Vibrio cholerae CTXφ are encoded by regions RS2. Mol. Microbiol. 1997; 24:917-26.Waldor M.K., Rubin E.J., Gregory D.N. et al. Regulation, replication, and integration functions of the Vibrio cholerae CTXφ are encoded by regions RS2. Mol. Microbiol. 1997; 24:917-26.

The authors declare that there are no conflicts of interest present.