microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2011-1(107)-58-61microbe-589Research ArticleМИКРОБИОЛОГИЯMICROBIOLOGYРазработка алгоритма MLST-типирования пандемических и предпандемических штаммов Vibrio cholerae биовара эльторElaboration of the Algorithm of MLST-Typing of Pandemic and Pre-Pandemic V. cholerae El Tor strainsОсинА. В.OssinA. V.microbe@san.ruКрасновЯ. М.KrasnovYa. M.noemail@neicon.ruГусеваН. П.GusevaN. P.noemail@neicon.ruСмирноваН. И.SmirnovaN. I.noemail@neicon.ruРоссийский научно-исследовательский противочумный институт «Микроб»РоссияRussian Research Anti-Plague Institute MicrobeRussian Federation20112002201101(107)5861Copyright © Осин А.В., Краснов Я.М., Гусева Н.П., Смирнова Н.И., 20112011Осин А.В., Краснов Я.М., Гусева Н.П., Смирнова Н.И.Ossin A.V., Krasnov Y.M., Guseva N.P., Smirnova N.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/589

Проведено молекулярное типирование пандемических и предпандемических штаммов холерного вибриона методом мультилокусного секвенирования. Применение двух схем MLST, основанных на сиквенсе генов, связанных с вирулентностью, и генов жизнеобеспечения, показало большую эффективность последней в дифференциации штаммов, выделенных до начала и в период 7-й пандемии холеры.

Molecular typing of pandemic and pre-pandemic cholera vibrio strains was carried out with the help of multi-locus sequence analysis. Application of two MLST schemes based on the sequence of virulence-associated and housekeeping genes demonstrated the last one to be the most effective in differentiation of strains, isolated before and during the seventh cholera pandemic.

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The authors declare that there are no conflicts of interest present.