microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2013-3-70-74microbe-64Research ArticleИММУНОЛОГИЯ, ПРОФИЛАКТИКАIMMUNOLOGY, PROPHYLAXISСоздание и изучение вариантов вакцинного штамма Francisella tularensis без генов iglC. Сообщение 1Construction and Investigation of the Vaccine Strain Francisella tularensis without iglC genes. Communication 1МокриевичА. Н.MokrievichA. N.info@obolensk.orgВахрамееваГ. М.VakhrameevaG. M.info@obolensk.orgМироноваР. И.MironovaR. I.info@obolensk.orgКомбароваТ. И.KombarovaT. I.info@obolensk.orgТитареваГ. М.TitarevaG. M.info@obolensk.orgКравченкоТ. Б.KravchenkoT. B.info@obolensk.orgБахтееваИ. В.BakhteevaI. V.info@obolensk.orgДятловИ. А.DyatlovI. A.info@obolensk.orgПавловВ. М.PavlovV. M.info@obolensk.orgГосударственный научный центр прикладной микробиологии и биотехнологииРоссияResearch Center for Applied Microbiology and BiotechnologyRussian Federation201320092013037074Copyright © Мокриевич А.Н., Вахрамеева Г.М., Миронова Р.И., Комбарова Т.И., Титарева Г.М., Кравченко Т.Б., Бахтеева И.В., Дятлов И.А., Павлов В.М., 20132013Мокриевич А.Н., Вахрамеева Г.М., Миронова Р.И., Комбарова Т.И., Титарева Г.М., Кравченко Т.Б., Бахтеева И.В., Дятлов И.А., Павлов В.М.Mokrievich A.N., Vakhrameeva G.M., Mironova R.I., Kombarova T.I., Titareva G.M., Kravchenko T.B., Bakhteeva I.V., Dyatlov I.A., Pavlov V.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/64

Получены варианты вакцинного штамма F. tularensis subsp. holarctic a 15 с делетированными генами iglC . Показано, что в геноме штамма 15 содержится две копии гена iglC . Удаление одной копии гена практически не влияет на культурально-морфологические и ростовые свойства микроба. Бактерии без одной копии гена iglC размножаются в мышиных макрофагах медленнее по сравнению с исходным штаммом, в то время как инактивация двух копий гена iglC в штамме 15 приводит к полной потере способности размножаться в макрофагах.

Using site-specific mutagenesis constructed were the variants of the vaccine strain F. tularensis subsp. holarctica 15 with the deleted iglC genes. Identified was the fact that genome of the strain 15 contained two copies of iglC gene. Deletion of one of them as well as both had little effect on the cultural-morphological and growth properties of the microbe. At the same time F. tularensis 15 lacking one copy of the iglC gene propagated in mice macrophages several times slower, than the original strain. Inactivation of both of the copies in the chromosome leaded to the emergence of a variant incapable of intracellular reproduction. This capacity in F. tularensis 15/23-2 with two inactivated iglC gene copies was partially recovered after integration of a complementing plasmid. Therewith the data mentioned above testifies to the significance of iglC gene for the process of reproduction in macrophages.

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The authors declare that there are no conflicts of interest present.