microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2011-3(109)-50-53microbe-680Research ArticleДИАГНОСТИКАDIAGNOSTICSПолучение иммуномагнитных частиц для определения клеток Francisella tularensisObtainment of the Immunomagnetic Particles for Francisella tularensis Cells DetectionВетчининС. С.VetchininS. S.info@obolensk.orgКопыловП. Х.KopylovP. Kh.noemail@neicon.ruКиселеваН. В.KiselevaN. V.noemail@neicon.ruНиколаеваО. Г.NikolaevaO. G.noemail@neicon.ruГаврюшкинА. В.GavryushkinA. V.noemail@neicon.ruГосударственный научный центр прикладной микробиологии и биотехнологииРоссияState Research Center for Applied Microbiology and BiotechnologyRussian Federation20112006201103(109)5053Copyright © Ветчинин С.С., Копылов П.Х., Киселева Н.В., Николаева О.Г., Гаврюшкин А.В., 20112011Ветчинин С.С., Копылов П.Х., Киселева Н.В., Николаева О.Г., Гаврюшкин А.В.Vetchinin S.S., Kopylov P.K., Kiseleva N.V., Nikolaeva O.G., Gavryushkin A.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/680

Получены иммуномагнитные частицы с иммобилизованными высокоспецифичными моноклональными антителами FB11-x к Francisella tularensis. Их использование в иммуноферментном анализе позволило определить 105 - 106 КОЕ / мл F. tularensis. Связывание бактериальных клеток с ИМЧ подтверждено флуоресцентной микроскопией.

Obtained are the immunomagnetic particles with the immobilized highly-specific monoclonal antibodies FB 11-x to Francisella tularensis. Their usage in enzyme-linked immunoassays made it possible to detect 105 - 106 CFU/ml of F. tularensis. The binding of the bacterial cells with IMP was confirmed by the results of fluorescent microscopy.

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The authors declare that there are no conflicts of interest present.