microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2011-3(109)-68-70microbe-685Research ArticleДИАГНОСТИКАDIAGNOSTICSРазработка мультианалитного иммунохроматографического теста для выявления ботулинических токсиновDevelopment of the Multi-Analyte Test for Immune-Chromatographic Detection of Botulinum ToxinsШиленкоИ. В.ShilenkoI. V.niibp@dol.ruТитовА. А.TitovA. A.noemail@neicon.ruЯрковС. П.YarkovS. P.noemail@neicon.ruЗлобинВ. Н.ZlobinV. N.noemail@neicon.ruГосударственный научно-исследовательский институт биологического приборостроенияРоссияState Research Institute of Biological Device EngineeringRussian Federation20112006201103(109)6870Copyright © Шиленко И.В., Титов А.А., Ярков С.П., Злобин В.Н., 20112011Шиленко И.В., Титов А.А., Ярков С.П., Злобин В.Н.Shilenko I.V., Titov A.A., Yarkov S.P., Zlobin V.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/685

Разработан мультианалитный тест на основе наночастиц коллоидного золота для одновременного выявления нейротоксинов (БТ) типов А и В, выделяемых Clostridium botulinum. Тест отличается наличием трех линий (двух тестовых и одной контрольной) в поле для регистрации результатов анализа. Тесты предполагается применять при анализе проб продуктов питания и проб, взятых из объектов окружающей среды. Чувствительность одновременного выявления БТ типов А (30 нг/мл) и В (10 нг/мл) не хуже, чем у моноаналитных тестов, сконструированных для выявления одного типа БТ. Показано, что тесты специфичны и могут быть применены для идентификации БТ в продуктах питания.

Designed is the multi-analyte test for simultaneous immune chromatographic detection of A & B type botulinum neurotoxins (BT), using colloid gold nanoparticles. It is meant for food and environmental samples' analysis. The sensitivity of simultaneous BT detection of the A (30 ng/ml) and B (10 ng/ml) types is as high as that of mono-analytical tests, designed for one type BT detection. The test is demonstrated to be a specific one and can be used for BT detection in food stuffs.

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