microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2011-4(110)-5-11microbe-694Research ArticleОБЗОРЫREVIEWSБактериальная биопленка и особенности ее образования у возбудителя чумы и других патогенных иерсинийBacterial Biofilm and Peculiarities of Its Formation in Plague Agent and in Other Pathogenic YersiniaКуклеваЛ. М.KuklevaL. M.rusrapi@microbe.ruЕрошенкоГ. А.EroshenkoG. A.noemail@neicon.ruВидяеваН. А.VidyaevaN. A.noemail@neicon.ruКутыревВ. В.KutyrevV. V.noemail@neicon.ruРоссийский научно-исследовательский противочумный институт «Микроб»РоссияRussian Research Anti-Plague Institute MicrobeRussian Federation20112008201104(110)511Copyright © Куклева Л.М., Ерошенко Г.А., Видяева Н.А., Кутырев В.В., 20112011Куклева Л.М., Ерошенко Г.А., Видяева Н.А., Кутырев В.В.Kukleva L.M., Eroshenko G.A., Vidyaeva N.A., Kutyrev V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/694

В обзоре представлены современные литературные данные об общих закономерностях образования бактериальных биопленок, этапах ее формирования и структурно-функциональной организации, а также об участии различных ферментных систем в функционировании биопленки. Проведен анализ сведений об особенностях образования биопленок патогенными иерсиниями - Yersinia pestis, Y. pseudotuberculosis и Y. enterocolitica. Представлены данные об участии генов hmsHFRS-оперона хромосомной области пигментации Y. pestis в формировании структуры биопленки и ее регуляции генами hmsP и hmsT. Обобщены результаты исследований последних лет, посвященных генетической детерминированности процессов образования биопленки возбудителем чумы, в частности участию генов синтеза липополисахарида (waaA, yrbH и gmhA) и полиаминов (speA и speС), а также регуляторных генов rcsA и phoP-phoQ. Приведены результаты собственных исследований по изучению особенностей формирования биопленки штаммами возбудителя чумы разных подвидов на абиотических поверхностях и модели нематоды Caenorhabditis elegans. Обсуждена роль биопленки в сложном жизненном цикле возбудителя чумы, отмечено ее значение не только для обеспечения трансмиссии чумы с помощью блох, но и для сохранения Y. pestis во внешней среде вне организма теплокровного хозяина.

This paper represents a review of the current literature data concerning general principles of bacterial biofilm formation, stages of biofilm production and its structural and functional organization, as well as the data concerning involvement of different enzyme systems with the process of biofilm functioning. Carried out is the analysis of the data on the peculiarities of biofilm formation by pathogenic Y. pestis, Y. pseudotuberculosis, and Y. enterocolitica. Displayed are the data on the role of hmsHFRS-operon genes, located in pigmentation chromosomal region of Y. pestis, in the process of biofilm structure formation, and the data on its regulation by hmsP and hmsT genes. Summarized are the results of the recent years' investigations devoted to the genetic determinancy of the plague agent biofilm formation processes, and in particular to the involvement of genes encoding synthesis of lipopolysaccharide (waaA, yrbH and gmhA) and polyamines (speA and speC), as well as rcsA and phoP-phoQ regulatory genes with biofilm formation procedures. Represented are the results of our own investigations concerning the studies of peculiarities of biofilm formation by plague agent strains of different subspecies on the abiotic surfaces and on the nematode model - Caenorhabditis elegans. Discussed is the role of the biofilm in the complex life span of the plague agent. Noted is biofilm significance not only for facilitation in plague transmission by fleas, but also for Y. pestis preservation in the environment, out of the organism of a warm-blooded host.

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The authors declare that there are no conflicts of interest present.