microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2011-2(108)-30-35microbe-744Research ArticleМИКРОБИОЛОГИЯMICROBIOLOGYОсобенности проявления признака пигментации и структурные различия генов hms-оперона у штаммов Y. pestis и Y. pseudotuberculosis разного происхожденияPeculiarities of Pigmentation Expression and Structural Differences of hms Operon Genes in Y. pestis and Y. pseudotuberculosis Strains of Diverse OriginБулгаковаЕ. Г.BoolgakovaE. G.microbe@san.ruКрасновЯ. М.KrasnovYa. M.noemail@neicon.ruГаеваА. В.GaevaA. V.noemail@neicon.ruСухоносовИ. Ю.SukhonosovI. Yu.noemail@neicon.ruАнисимоваЛ. В.AnisimovaL. V.noemail@neicon.ruГусеваН. П.GusevaN. P.noemail@neicon.ruНовичковаЛ. А.NovichkovaL. A.noemail@neicon.ruКутыревВ. В.KutyrevV. V.noemail@neicon.ruРоссийский научно-исследовательский противочумный институт «Микроб»РоссияRussian Research Anti-Plague Institute MicrobeRussian Federation20112004201102(108)3035Copyright © Булгакова Е.Г., Краснов Я.М., Гаева А.В., Сухоносов И.Ю., Анисимова Л.В., Гусева Н.П., Новичкова Л.А., Кутырев В.В., 20112011Булгакова Е.Г., Краснов Я.М., Гаева А.В., Сухоносов И.Ю., Анисимова Л.В., Гусева Н.П., Новичкова Л.А., Кутырев В.В.Boolgakova E.G., Krasnov Y.M., Gaeva A.V., Sukhonosov I.Y., Anisimova L.V., Guseva N.P., Novichkova L.A., Kutyrev V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/744

Проведено исследование признака пигментации у 214 штаммов чумного микроба и 68 псевдотуберкулезного микроба. Выявлено, что штаммы чумного микроба разных подвидов и псевдотуберкулезного микроба проявляют разную способность к сорбции гемина и кислых красителей. Проведен ПЦР-анализ и определение нуклеотидной последовательности генов hms-оперона у типовых штаммов пяти подвидов и таласской группы штаммов чумного микроба и штамма O1 серотипа псевдотуберкулезного микроба. Выявлены единичные нуклеотидные замены во всех генах оперона штаммов чумного микроба по сравнению с опероном штаммов псевдотуберкулезного микроба. Определены единичные нуклеотидные замены, перспективные для внутривидовой дифференциации штаммов чумного микроба.

Pigmentation was examined in 214 plague microbe strains and 68 pseudotuberculosis agent strains. Plague microbe strains of different subspecies and pseudotuberculosis microbe strains demonstrated different ability of hemin and acidic dyes sorption. Genes of hms operon in typical Y. pestis strains of five subspecies and in talassica group strains, as well as in Y. pseudotuberculosis O1 serotype strains, were sequenced and analyzed using PCR. Single nucleotide substitutions were detected in all genes of the operon in plague microbe strains as compared with the operon of pseudotuberculosis agent strains. Determined were single nucleotide substitutions promising for intraspecific differentiation of plague microbe strains.

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The authors declare that there are no conflicts of interest present.