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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">microbe</journal-id><journal-title-group><journal-title xml:lang="ru">Проблемы особо опасных инфекций</journal-title><trans-title-group xml:lang="en"><trans-title>Problems of Particularly Dangerous Infections</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0370-1069</issn><issn pub-type="epub">2658-719X</issn><publisher><publisher-name>Russian Research Anti-Plague Institute “Microbe”</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21055/0370-1069-2012-2(112)-46-50</article-id><article-id custom-type="elpub" pub-id-type="custom">microbe-794</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ДИАГНОСТИКА</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>DIAGNOSTICS</subject></subj-group></article-categories><title-group><article-title>Современные подходы к диагностике сапа и мелиоидоза. Идентификация и типирование Burkholderia mallei и Burkholderia pseudomallei</article-title><trans-title-group xml:lang="en"><trans-title>Modern Approaches for Detection of Glanders and Melioidosis. Identification and Typing of Burkholderia mallei and Burkholderia pseudomallei</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Антонов</surname><given-names>В. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Antonov</surname><given-names>V. A.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Илюхин</surname><given-names>В. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Ilyukhin</surname><given-names>V. I.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Храпова</surname><given-names>Н. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Khrapova</surname><given-names>N. P.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Прохватилова</surname><given-names>Е. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Prokhvatilova</surname><given-names>E. V.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Викторов</surname><given-names>Д. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Viktorov</surname><given-names>D. V.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сенина</surname><given-names>Т. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Senina</surname><given-names>T. V.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Будченко</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Budchenko</surname><given-names>A. A.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ткаченко</surname><given-names>Г. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Tkachenko</surname><given-names>G. A.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Алексеева</surname><given-names>В. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Alekseeva</surname><given-names>V. V.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Захарова</surname><given-names>И. Б.</given-names></name><name name-style="western" xml:lang="en"><surname>Zakharova</surname><given-names>I. B.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Савченко</surname><given-names>С. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Savchenko</surname><given-names>S. S.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Зинченко</surname><given-names>О. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Zinchenko</surname><given-names>O. V.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Сорокина</surname><given-names>Ю. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Sorokina</surname><given-names>Yu. I.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Алексеев</surname><given-names>В. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Alekseev</surname><given-names>V. V.</given-names></name></name-alternatives><email xlink:type="simple">vari2@sprint-v.com.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Волгоградский научно-исследовательский противочумный институт</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Volgograd Research Anti-Plague Institute</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2012</year></pub-date><pub-date pub-type="epub"><day>20</day><month>04</month><year>2012</year></pub-date><volume>0</volume><issue>2(112)</issue><fpage>46</fpage><lpage>50</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Антонов В.А., Илюхин В.И., Храпова Н.П., Прохватилова Е.В., Викторов Д.В., Сенина Т.В., Будченко А.А., Ткаченко Г.А., Алексеева В.В., Захарова И.Б., Савченко С.С., Зинченко О.В., Сорокина Ю.И., Алексеев В.В., 2012</copyright-statement><copyright-year>2012</copyright-year><copyright-holder xml:lang="ru">Антонов В.А., Илюхин В.И., Храпова Н.П., Прохватилова Е.В., Викторов Д.В., Сенина Т.В., Будченко А.А., Ткаченко Г.А., Алексеева В.В., Захарова И.Б., Савченко С.С., Зинченко О.В., Сорокина Ю.И., Алексеев В.В.</copyright-holder><copyright-holder xml:lang="en">Antonov V.A., Ilyukhin V.I., Khrapova N.P., Prokhvatilova E.V., Viktorov D.V., Senina T.V., Budchenko A.A., Tkachenko G.A., Alekseeva V.V., Zakharova I.B., Savchenko S.S., Zinchenko O.V., Sorokina Y.I., Alekseev V.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://journal.microbe.ru/jour/article/view/794">https://journal.microbe.ru/jour/article/view/794</self-uri><abstract><p>Проанализированы методические подходы, используемые для идентификации и типирования B. mallei и B. pseudomallei, предложены рекомендации по совершенствованию алгоритмов лабораторной диагностики сапа и мелиоидоза с учетом широкого спектра биохимических, иммунодиагностических и молекулярно-генетических тестов.</p></abstract><trans-abstract xml:lang="en"><p>Analysed are the methodological approaches used for identification and typing of B. mallei and B. pseudomallei. Suggested are recommendations for improvements of algorithms of laboratory diagnosis of glanders and melioidosis including wide range of biochemical, immunodiagnostic and molecular genetic methods.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>сап</kwd><kwd>мелиоидоз</kwd><kwd>генетическое типирование</kwd><kwd>диагностика</kwd><kwd>полимеразная цепная реакция</kwd></kwd-group><kwd-group xml:lang="en"><kwd>glanders</kwd><kwd>melioidosis</kwd><kwd>genetic typing</kwd><kwd>diagnostic</kwd><kwd>polymerase chain reaction</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Алтухова В.В., Антонов В.А., Ткаченко Г.А. и др. Использование полимеразной цепной реакции для обнаружения возбудителей сапа и мелиоидоза при экспериментальной инфекции. 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