microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2012-2(112)-59-61microbe-797Research ArticleДИАГНОСТИКАDIAGNOSTICSКонструирование праймеров для детекции и типирования генов β-лактамаз патогенных видов рода BurkholderiaDesign of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of BurkholderiaРомановаА. В.RomanovaA. V.vari2@sprint-v.com.ruЗахароваИ. Б.ZakharovaI. B.vari2@sprint-v.com.ruЗамараевВ. С.ZamaraevV. S.vari2@sprint-v.com.ruВикторовД. В.ViktorovD. V.vari2@sprint-v.com.ruВолгоградский научно-исследовательский противочумный институтРоссияVolgograd Research Anti-Plague InstituteRussian Federation20122004201202(112)5961Copyright © Романова А.В., Захарова И.Б., Замараев В.С., Викторов Д.В., 20122012Романова А.В., Захарова И.Б., Замараев В.С., Викторов Д.В.Romanova A.V., Zakharova I.B., Zamaraev V.S., Viktorov D.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/797

Сконструирован набор олигонуклеотидных праймеров для проведения экспресс-оценки наличия детерминант устойчивости к β-лактамам у изолятов патогенных буркхольдерий методом полимеразной цепной реакции (ПЦР) с одновременным определением принадлежности выявленных β-лактамаз к определенному молекулярному классу. ПЦР с праймерами, специфичными последовательностям генов металло-β-лактамаз (класс В β-лактамазы), и оксациллиназ семейства D-ala карбоксипептидаз (класс D β-лактамазы) позволяет дифференцировать между собой виды Burkholderia группы «pseudomallei».

The set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of D-ala carboxypeptidase family (class D of β-lactamase) gene sequences allowed to differentiate among pseudomallei group of Burkholderia species.

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The authors declare that there are no conflicts of interest present.