microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2012-2(112)-89-93microbe-805Research ArticleБИОТЕХНОЛОГИЯBIOTECHNOLOGYРазработка ДНК-биочипа для выявления субтипов вируса гриппа АDevelopment of DNA-Biochip for Identification of Influenza A Virus SubtypesШиковА. Н.ShikovA. N.vector@vector.nsc.ruСергееваЕ. И.SergeevaE. I.vector@vector.nsc.ruДеминаО. К.DeminaO. K.vector@vector.nsc.ruТерновойВ. А.TernovoyV. A.vector@vector.nsc.ruРябининВ. В.RyabininV. V.noemail@neicon.ruКостинаЕ. В.KostinaE. V.noemail@neicon.ruМалковаЕ. М.MalkovaE. M.vector@vector.nsc.ruСиняковА. Н.SinyakovA. N.noemail@neicon.ruАгафоновА. П.AgaphonovA. P.vector@vector.nsc.ruГосударственный научный центр вирусологии и биотехнологии «Вектор»РоссияState Research Centre of Virology and Biotechnology VectorRussian FederationНовосибирский институт химической биологии и фундаментальной медицины СО РАНРоссияNovosibirsk Institute of Chemical Biology and Fundamental MedicineRussian Federation20122004201202(112)8993Copyright © Шиков А.Н., Сергеева Е.И., Демина О.К., Терновой В.А., Рябинин В.В., Костина Е.В., Малкова Е.М., Синяков А.Н., Агафонов А.П., 20122012Шиков А.Н., Сергеева Е.И., Демина О.К., Терновой В.А., Рябинин В.В., Костина Е.В., Малкова Е.М., Синяков А.Н., Агафонов А.П.Shikov A.N., Sergeeva E.I., Demina O.K., Ternovoy V.A., Ryabinin V.V., Kostina E.V., Malkova E.M., Sinyakov A.N., Agaphonov A.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/805

Разработан ДНК-биочип для определения субтипов вируса гриппа А, патогенных для человека. Микрочип способен определять H1, H3, H5-субтипы гемагглютинина (включая H1-субтип гемагглютинина пандемического вируса гриппа A/H1N1(2009)) и N1, N2-субтипы нейраминидазы вируса гриппа. Полученный микрочип был успешно апробирован на штаммах высокопатогенного вируса гриппа птиц A/H5N1, пандемического вируса гриппа A/H1N1(2009), сезонных вирусах гриппа A/H1N1 и A/H3N2.

Developed was the DNA-biochip to identify subtypes of influenza A virus, pathogenic for humans. Microchip was capable of detecting H1, H3, H5-subtypes of hemagglutinin (including H1-subtype of pandemic A/H1N1(2009) influenza virus ) and neuraminidase subtypes N1,N2 of influenza virus. This microchip was successfully tested on the strains of A/H5N1 highly pathogenic avian influenza virus, A/H1N1(2009) pandemic influenza virus, A/H1N1 and A/H3N2 seasonal influenza viruses.

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The authors declare that there are no conflicts of interest present.