microbeПроблемы особо опасных инфекцийProblems of Particularly Dangerous Infections0370-10692658-719XRussian Research Anti-Plague Institute “Microbe”10.21055/0370-1069-2013-4-89-94microbe-86Research ArticleДИАГНОСТИКАDIAGNOSTICSСовременное состояние лабораторной диагностики лихорадки денге (обзор)Current State of Dengue Fever Laboratory Diagnostics (Scientific Review)НайденоваЕ. В.NaydenovaE. V.rusrapi@microbe.ruКуклевВ. Е.KuklevV. E.rusrapi@microbe.ruЯшечкинЮ. И.YashechkinYu. I.rusrapi@microbe.ruЩербаковаС. А.ShcherbakovaS. A.rusrapi@microbe.ruКутыревВ. В.KutyrevV. V.rusrapi@microbe.ruРоссийский научно-исследовательский противочумный институт «Микроб»РоссияRussian Research Anti-Plague Institute “Microbe”Russian Federation201320122013048994Copyright © Найденова Е.В., Куклев В.Е., Яшечкин Ю.И., Щербакова С.А., Кутырев В.В., 20132013Найденова Е.В., Куклев В.Е., Яшечкин Ю.И., Щербакова С.А., Кутырев В.В.Naydenova E.V., Kuklev V.E., Yashechkin Y.I., Shcherbakova S.A., Kutyrev V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://journal.microbe.ru/jour/article/view/86

В работе приведены современные данные о распространении, строении и классификации вирусов Денге и лабораторной диагностике вызываемых ими заболеваний с использованием классических и современных вирусологических методов, рассматривается возможность применения различных лабораторных тестов в разные сроки от начала заболевания. Участившиеся в настоящее время случаи завоза данного инфекционного заболевания на территорию Российской Федерации показывают необходимость разработки тест-систем отечественного производства, а также внедрения в практику противочумных институтов технологий выделения вирусов на биологической модели или культуре клеток.

The paper contains the data on the distribution, morphology and classification of Dengue viruses as well as laboratory diagnostics of the diseases caused by them using conventional and modern virology methods. Discussed is the possibility of application of various laboratory techniques at different stages from the onset of the disease. In addition, up to date cases of Dengue fever importation into the territory of the Russian Federation are becoming more frequent which testifies to the need of the development of domestically produced test-system, as well as introduction of modern technologies of virus isolation on the biological model or cell culture into the practice of plague control institutions.

вирусы Денгелихорадка денгелабораторная диагностикаDengue virusesDengue feverlaboratory diagnosticsReferencesГанушкина Л.А., Дремова В.П. Комары Aedes aegypti L. и Aedes albopictus skuse – новая биологическая угроза для юга России. Мед. паразитол. и паразитарн. бол. 2012; 3: 49– 55.Ganushkina L.A., Dremova V.P. [Mosquitoes Aedes aegypti L. and Aedes albopictus skuse – emerging biological threat to the public health of the population in the southern Russia]. Med. Parazitol. Parazitarn. Bol. 2012; 3:49–55.Ганушкина Л.А., Таныгина Е.Ю., Безжонова О.В., Сергиев В.П. Об обнаружении комаров Aedes (Stegomyia) albopictus skup. на территории Российской Федерации. Мед. паразитол. и паразитарн. бол. 2012; 1:3–4.Ganushkina L.A., Tanygina E.Yu., Bezzhonova O.V., Sergiev V.P. [Concerning mosquito detection (Aedes (Stegomyia) albopictus skup.) in the territory of the Russian Federation]. Med. Parazitol. Parazitarn. Bol. 2012; 1:3–4.Ларичев В.Ф., Сайфуллин М.А., Акиншина Ю.А., Хуторецкая Н.В., Бутенко А.М. Завозные случаи арбовирусных инфекций в Российской Федерации. Эпидемиол. и инф. бол. 2012; 1:35–9.Larichev V.F., Sayfullin M.A., Akinshina Yu.A., Khutoretskaya N.V., Butenko A.M. [Imported cases of Arboviral infections in the Russian Federation]. Epidemiol. Infek. Bol. 2012; 1:35–9.Львов Д.К., редактор. Медицинская вирусология: Руководство. М.: МИА; 2008. 655 с.L’vov D.K., editor [Medical virology: practice guidelines]. M.: MIA; 2008. 655 p.Львов Д.К., Клименко С.М., Гайдамович С.Я. Арбовирусы и арбовирусные инфекции. М: Медицина; 1989. 336 с.L’vov D.K., Klimenko S.M., Gaydamovich S.Ya. [Arboviruses and Arboviral Infections]. M.: Meditsina: 1989. 336 p.Найденова Е.В., Яшечкин Ю.И., Щербакова С.А. Набор реагентов для выявления РНК вирусов Денге (I–IV типов) методом обратной транскрипции и полимеразной цепной реакции. Национальные приоритеты России, Спец. выпуск. 2011; 2(5):153.Naydenova E.V., Yashechkin Yu.I., Shcherbakova S.A. [Reagent panel for Dengue virus RNA detection (I–IV types) by means of reverse transcription and polymerase chain reaction] Natsional. Prioritety Rossii. Special Issue. 2011; 2(5): 153.Онищенко Г.Г., редактор. Специфическая индикация патогенных биологических агентов: Практическое руководство. М.:ЗАО «МП Гигиена»; 2006. 288 с.Onishchenko G.G., editor [Specific Indication of Pathogenic Biological Agents: Practice Guidelines]. M.: ZAO “MP Gigiena”; 2006. 288 p.Покровский В.И., редактор. Инфекционные болезни: Руководство для врачей. М.: «Медицина»; 1996. 528 с.Pokrovsky V.I., editor [Infectious Diseases: Guidelines for Physicians]. M.: Meditsina; 1996. 528 p.Рябова Т.Е., Юничева Ю.В., Маркович Н.Я., Ганушкина Л.А., Орабей В.Г., Сергиев В.П. Обнаружение комаров Aedes (Stegomyia) aegypti L. в г. Сочи. Мед. паразитол. и паразитарн. бол. 2005; 3:3–5.Ryabova T.E., Yunicheva Yu.V., Markovich N.Ya., Ganushkina L.A., Orabey V.G., Sergiev V.P. [Mosquito detection (Aedes (Stegomyia) aegypti L..) in Sochi]. Med. Parazitol. Parazitarn. Bol. 2005; 3: 3–5.Юничева Ю.В., Рябова Т.Е., Маркович Н.Я., Безжонова О.В., Ганушкина Л.А., Семенов В.Б. Первые данные о наличии размножающейся популяции комаров Aedes aegypti в районе Большого Сочи и в отдельных городах Абхазии. Мед. паразитол. и паразитарн. бол. 2008;3:40–43.Yunicheva Yu.V., Ryabova T.E., Markovich N.Ya., Bezzhonova O.V., Ganushkina L.A., Semenov V.B. [First records of the proliferating mosquito population (Aedes aegypti) in the vicinity of the Greater Sochi and in several towns of Abkhazia]. Med. Parazitol. Parazitarn. Bol. 2008; 3: 40–43.Alfonso H.L., Amarilla A.A., Gonçalves P., Barros M.T., de Almeida F., Silva T.R., Nunes M.T., Vasconcelos P.F.C., Vieira D.S., Batista W.C., Bobadilla M.L., Vazquez C., Moran M., Figueiredo L.T.M., Aquino V.H. Pylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics. Virol. J. 2012, 9:124.Alfonso H.L., Amarilla A.A., Gonçalves P., Barros M.T., de Almeida F., Silva T.R., Nunes M.T., Vasconcelos P.F.C., Vieira D.S., Batista W.C., Bobadilla M.L., Vazquez C., Moran M., Figueiredo L.T.M., Aquino V.H. Pylogenetic relationship of dengue virus type 3 isolated in Brazil and Paraguay and global evolutionary divergence dynamics. Virol. J. 2012, 9:124.Avilés G., Paz M.V., Rangeon G., Ranaivoarisoa M.Y., Verzeri N., Roginski S. Laboratory surveillance of dengue in Argentina, 1995–2001. Emerg. Infect. Dis. 2003; 9:738–42.Avilés G., Paz M.V., Rangeon G., Ranaivoarisoa M.Y., Verzeri N., Roginski S. Laboratory surveillance of dengue in Argentina, 1995–2001. Emerg. Infect. Dis. 2003; 9:738–42.Blacksell S.D., Jarman R.G., Gibbons R.V., Tanganuchitcharnchai A., Mammen M.P. Jr., Nisalak A., Kalayanarooj S., Bailey M.S., Premaratna R., de Silva H., Day N.P.J., Lalloo D.G. Comparison of seven commercial antigen and antibody detection ELISAs for acute dengue infection. Clin. Vaccine Immunol. 2012; 19(5):804–10.Blacksell S.D., Jarman R.G., Gibbons R.V., Tanganuchitcharnchai A., Mammen M.P. Jr., Nisalak A., Kalayanarooj S., Bailey M.S., Premaratna R., de Silva H., Day N.P.J., Lalloo D.G. Comparison of seven commercial antigen and antibody detection ELISAs for acute dengue infection. Clin. Vaccine Immunol. 2012; 19(5):804–10.Bona A.C.D., Twerdochlib A.L., Navarro-Silva M.A. Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region. Rev. Soc. Bras. Med. Trop. 2012; 45(3):297–300.Bona A.C.D., Twerdochlib A.L., Navarro-Silva M.A. Genetic diversity of dengue virus serotypes 1 and 2 in the State of Paraná, Brazil, based on a fragment of the capsid/premembrane junction region. Rev. Soc. Bras. Med. Trop. 2012; 45(3):297–300.Cordeiro M., Braga-Neto U., Nogueira R.M., Ernesto T.A., Marques Jr. Reliable classifier to differentiate primary and secondary acute Dengue infection based on IgG ELISA. Virology. 2008; 2(8):280.Cordeiro M., Braga-Neto U., Nogueira R.M., Ernesto T.A., Marques Jr. Reliable classifier to differentiate primary and secondary acute Dengue infection based on IgG ELISA. Virology. 2008; 2(8):280.Dash P.K., Parida M., Santhosh S.R., Saxena P., Srivastava A., Neeraja M., Neeraja M., Lakshmi V., Rao L. Development and evaluation of a 1-step duplex reverse transcription polymerase chain reaction for differential diagnosis of chikungunya and dengue infection. Diagn. Мicrobiol. Infect. Dis. 2008; 62(1):52–7.Dash P.K., Parida M., Santhosh S.R., Saxena P., Srivastava A., Neeraja M., Neeraja M., Lakshmi V., Rao L. Development and evaluation of a 1-step duplex reverse transcription polymerase chain reaction for differential diagnosis of chikungunya and dengue infection. Diagn. Мicrobiol. Infect. Dis. 2008; 62(1):52–7.Dussart P., Petit L., Labeau B., Bremand L., Leduc A., Moua D., Matheus S., Baril L. Evaluation of Two New Commercial Tests for the Diagnosis of Acute Dengue Virus Infection Using NS1 Antigen Detection in Human Serum. PLoS Negl. Trop. Dis. 2008; 2(8):e280.Dussart P., Petit L., Labeau B., Bremand L., Leduc A., Moua D., Matheus S., Baril L. Evaluation of Two New Commercial Tests for the Diagnosis of Acute Dengue Virus Infection Using NS1 Antigen Detection in Human Serum. PLoS Negl. Trop. Dis. 2008; 2(8):e280.Gittens-St Hilaire M., Clarke-Greenidge N. An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction. Virol. J. 2008, 5:152.Gittens-St Hilaire M., Clarke-Greenidge N. An analysis of the subtypes of dengue fever infections in Barbados 2003–2007 by reverse transcriptase polymerase chain reaction. Virol. J. 2008, 5:152.Gjenero-Margan I., Aleraj B., Krajcar D., Lesnikar V., Klobucar A., Kurecic-Filipovic S., Komparak S., Martić R., Duričić S., Betica-Radić L., Okmadžić J., Vilibić-Čavlek T., Babić-Erceg A., Turković B., Avsić-Županc T., Radić I., Ljubić M., Sarac K., Benić N., Mlinarić-Galinović G. Autochthonous dengue fever in Croatia, August-September 2010. Euro Surveill. 2011; 16. pii 19805.Gjenero-Margan I., Aleraj B., Krajcar D., Lesnikar V., Klobucar A., Kurecic-Filipovic S., Komparak S., Martić R., Duričić S., Betica-Radić L., Okmadžić J., Vilibić-Čavlek T., Babić-Erceg A., Turković B., Avsić-Županc T., Radić I., Ljubić M., Sarac K., Benić N., Mlinarić-Galinović G. Autochthonous dengue fever in Croatia, August-September 2010. Euro Surveill. 2011; 16. pii 19805.Gurukumar K.R., Priyadarshini D., Patil J.A., Bhagat A., Singh A., Shah P.S., Cecilia D. Development of real time PCR for detection and quantitation of Dengue Viruses. Virol. J. 2009; 6:10.Gurukumar K.R., Priyadarshini D., Patil J.A., Bhagat A., Singh A., Shah P.S., Cecilia D. Development of real time PCR for detection and quantitation of Dengue Viruses. Virol. J. 2009; 6:10.Hang V.T., Nguyet N.M., Trung D., Tricou V., Yoksan S., Dung N.M., Van Ngoc T., Hien T.T., Farrar J., Wills B., Simmons C. Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid. PLoS Negl. Trop. Dis. 2009; 3(1): e360.Hang V.T., Nguyet N.M., Trung D., Tricou V., Yoksan S., Dung N.M., Van Ngoc T., Hien T.T., Farrar J., Wills B., Simmons C. Tests for Dengue Sensitivity, Specificity and Relationship to Viraemia and Antibody Responses Diagnostic Accuracy of NS1 ELISA and Lateral Flow Rapid. PLoS Negl. Trop. Dis. 2009; 3(1): e360.Huhtamo E., Uzcátegui N.Y., Siikamäki H. Molecular epidemiology of dengue virus strains from Finnish travelers. Emerg. Infect. Dis. 2008;14(1):80–3.Huhtamo E., Uzcátegui N.Y., Siikamäki H. Molecular epidemiology of dengue virus strains from Finnish travelers. Emerg. Infect. Dis. 2008;14(1):80–3.Ito M., Takasaki T., Yamada K., Nerome R., Tajima S., Kurane I. Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4. J. Clin. Microbiol. 2004; 42(12):5935–7.Ito M., Takasaki T., Yamada K., Nerome R., Tajima S., Kurane I. Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4. J. Clin. Microbiol. 2004; 42(12):5935–7.Klungthong C., Putnak R., Mammena M.P., Zhang C. Molecular genotyping of dengue viruses by phylogenetic analysis of the sequences of individual genes. J. Virol. Methods. 2008; 154:175–81.Klungthong C., Putnak R., Mammena M.P., Zhang C. Molecular genotyping of dengue viruses by phylogenetic analysis of the sequences of individual genes. J. Virol. Methods. 2008; 154:175–81.Klunghong G., Gibbons R.V., Thaisomboonsuk B., Nisalak A., Kalayanarooj S., Thirawuth V. Dengue virus detection using whole blood for reverse transcriptase PCR and virus isolation. J. Clin. Mirobiol. 2007; 8:2480–5.Klunghong G., Gibbons R.V., Thaisomboonsuk B., Nisalak A., Kalayanarooj S., Thirawuth V. Dengue virus detection using whole blood for reverse transcriptase PCR and virus isolation. J. Clin. Mirobiol. 2007; 8:2480–5.Kong Y.Y., Thay C.H., Tin T.C., Devi S. Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR. J. Virol. Methods. 2006; 138:123–30.Kong Y.Y., Thay C.H., Tin T.C., Devi S. Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR. J. Virol. Methods. 2006; 138:123–30.Kuno G., Gubler D.J., Vélez M., Oliver A. Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses. Bull. World Health Organ. 1985; 63(2):279–86.Kuno G., Gubler D.J., Vélez M., Oliver A. Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses. Bull. World Health Organ. 1985; 63(2):279–86.La Ruche G., Souares Y., Armengaud A., Peloux-Petiot F., Delaunay P., Despres P., Lenglet A., Jourdain F., Leparc-Goffart I., Charlet F., Ollier L., Mantey K., Mollet T., Fournier J.P., Torrents R., Leitmeyer K., Hilairet P., Zeller H., Van Bortel W., Dejour-Salamanca D., Grandadam M., Gastellu-Etchegorry M. First two autochthonous dengue virus infections in metropolitan France, September 2010. Euro Surveill. 2010; 15. pii19676.La Ruche G., Souares Y., Armengaud A., Peloux-Petiot F., Delaunay P., Despres P., Lenglet A., Jourdain F., Leparc-Goffart I., Charlet F., Ollier L., Mantey K., Mollet T., Fournier J.P., Torrents R., Leitmeyer K., Hilairet P., Zeller H., Van Bortel W., Dejour-Salamanca D., Grandadam M., Gastellu- Etchegorry M. First two autochthonous dengue virus infections in metropolitan France, September 2010. Euro Surveill. 2010; 15. pii19676.Lanciotti R.S., Calisher C.H., Gubler D.J., Chang G.J., Vorndam A.V. Rapid Detection and Typing of Dengue Viruses from Clinical Samples by Using Reverse Transcriptase-Polymerase Chain Reaction. J. Clin. Microbiol. 1992; 30(3):545–51.Lanciotti R.S., Calisher C.H., Gubler D.J., Chang G.J., Vorndam A.V. Rapid Detection and Typing of Dengue Viruses from Clinical Samples by Using Reverse Transcriptase-Polymerase Chain Reaction. J. Clin. Microbiol. 1992; 30(3):545–51.Levi J.E., Tateno A.F., Machado A.F., Ramalho D.C., de Souza V.A., Guilarde A.O., de Rezende Feres V.C., Martelli C.M., Turchi M.D., Siqueira J.B. Jr., Pannuti C.S. Evaluation of a commercial real-time PCR kit for detection of Dengue Virus in samples collected during an outbreak in Goiânia, Central Brazil, in 2005. J. Clin. Mirobiol. 2007; 6:1893–97.Levi J.E., Tateno A.F., Machado A.F., Ramalho D.C., de Souza V.A., Guilarde A.O., de Rezende Feres V.C., Martelli C.M., Turchi M.D., Siqueira J.B. Jr., Pannuti C.S. Evaluation of a commercial real-time PCR kit for detection of Dengue Virus in samples collected during an outbreak in Goiânia, Central Brazil, in 2005. J. Clin. Mirobiol. 2007; 6:1893–97.Li S., Fang M., Zhou B., Ni H., Shen O., Zhang H., Han Y., Yin J., Chang W., Xu G., Cao G. Simultaneous detection and differentiation of dengue virus serotypes 1–4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay. Virol. J. 2011; 8:360.Li S., Fang M., Zhou B., Ni H., Shen O., Zhang H., Han Y., Yin J., Chang W., Xu G., Cao G. Simultaneous detection and differentiation of dengue virus serotypes 1–4, Japanese encephalitis virus, and West Nile virus by a combined reverse-transcription loop-mediated isothermal amplification assay. Virol. J. 2011; 8:360.Louis C. Daily Newspaper View of Dengue Fever Epidemic, Athens, Greece, 1927–1931. Emerg. Infect. Dis. 2012; 1:78–82.Louis C. Daily Newspaper View of Dengue Fever Epidemic, Athens, Greece, 1927–1931. Emerg. Infect. Dis. 2012; 1:78–82.Menting S., Khoa T.D., Tran T.T., Hoang L.P., Klatser P., Wolthers K.C., Binh T.Q., de Vries P.J., Beld M. Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections. Adv. Virol. 2011; 2011:514681. doi: 10.1155/2011/514681.Menting S., Khoa T.D., Tran T.T., Hoang L.P., Klatser P., Wolthers K.C., Binh T.Q., de Vries P.J., Beld M. Internally Controlled, Generic Real-Time PCR for Quantification and Multiplex Real-Time PCR with Serotype-Specific Probes for Serotyping of Dengue Virus Infections. Adv. Virol. 2011; 2011:514681. doi: 10.1155/2011/514681.Messer W.B., Gubler D.J., Harris E., Sivananthan K., de Silva A.M. Emergence and global spread of a dengue serotype 3, subtype III virus. Emerg. Infect. Dis. 2003; 9(7):800–9.Messer W.B., Gubler D.J., Harris E., Sivananthan K., de Silva A.M. Emergence and global spread of a dengue serotype 3, subtype III virus. Emerg. Infect. Dis. 2003; 9(7):800–9.Mishra B., Sharma M., Pujhari S.K., Ratho R.K., Gopal S., Kumar N., Sarangi G., Chayani N., Varma S.C. Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples. Diagn. Microbiol. Infect. Dis. 2011; 71(2):118–25.Mishra B., Sharma M., Pujhari S.K., Ratho R.K., Gopal S., Kumar N., Sarangi G., Chayani N., Varma S.C. Utility of multiplex reverse transcriptase-polymerase chain reaction for diagnosis and serotypic characterization of dengue and chikungunya viruses in clinical samples. Diagn. Microbiol. Infect. Dis. 2011; 71(2):118–25.Mohammed H.P., Ramos M.M., Rivera A., Johansson M., Muñoz-Jordan J.L., Sun W., Tomashek K.M. Travel-associated dengue infections in the United States, 1996 to 2005. J. Travel Med. 2010; 17(1):8–14.Mohammed H.P., Ramos M.M., Rivera A., Johansson M., Muñoz- Jordan J.L., Sun W., Tomashek K.M. Travel-associated dengue infections in the United States, 1996 to 2005. J. Travel Med. 2010; 17(1):8–14.Narvaez F., Gutierrez G., Perez M.A., Elizondo D., Nunez A., Balmaseda A. Harris E. Evaluation of the Traditional and Revised WHO Classifications of Dengue Disease Severity. PLoS Negl. Trop. Dis. 2011; 5(11):1397.Narvaez F., Gutierrez G., Perez M.A., Elizondo D., Nunez A., Balmaseda A. Harris E. Evaluation of the Traditional and Revised WHO Classifications of Dengue Disease Severity. PLoS Negl. Trop. Dis. 2011; 5(11):1397.Nicoletti L., Ciccozzi M. Chikungunya and Dengue Viruses in Travelers. Emerg. Infect. Dis. 2008; 1:177–8.Nicoletti L., Ciccozzi M. Chikungunya and Dengue Viruses in Travelers. Emerg. Infect. Dis. 2008; 1:177–8.Parida M., Horioke K., Ishida H., Dash P.K., Saxena P., Jana A.M., Islam M.A., Inoue S., Hosaka N., Morita K. Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real-Time Reverse Transcription-Loop-Mediated Isothermal. J. Clin. Mirobiol. 2005; 43(6):2895–903.Parida M., Horioke K., Ishida H., Dash P.K., Saxena P., Jana A.M., Islam M.A., Inoue S., Hosaka N., Morita K. Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real-Time Reverse Transcription-Loop-Mediated Isothermal. J. Clin. Mirobiol. 2005; 43(6):2895–903.Potiwat R., Komalamisra N., Thavara U., Tawatsin A., Siriyasatien P. Competitive suppression between chikungunya and dengue virus in Aedes albopictus c6/36 cell line. Southeast Asian J. Trop. Med. Public Health. 2011; 42(6):1388–94.Potiwat R., Komalamisra N., Thavara U., Tawatsin A., Siriyasatien P. Competitive suppression between chikungunya and dengue virus in Aedes albopictus c6/36 cell line. Southeast Asian J. Trop. Med. Public Health. 2011; 42(6):1388–94.Rocco I.M., Silveira V.R., Maeda A.Y., dos Santos Silva S.J., Spenassatto C., Bisordi I., Suzuki A. First Isolation of Dengue 4 in the State of San Paulo, Brazil, 2011. Rev. Inst. Med. Trop. 2012; 54(1):49–51.Rocco I.M., Silveira V.R., Maeda A.Y., dos Santos Silva S.J., Spenassatto C., Bisordi I., Suzuki A. First Isolation of Dengue 4 in the State of San Paulo, Brazil, 2011. Rev. Inst. Med. Trop. 2012; 54(1):49–51.Saxena P., Kumar Dash P., Sanhosh S.R. Development and evaluation of one step single tube multiplex PT-PCR for rapid detection and typing of dengue viruses. Virol. J. 2008; 5:20.Saxena P., Kumar Dash P., Sanhosh S.R. Development and evaluation of one step single tube multiplex PT-PCR for rapid detection and typing of dengue viruses. Virol. J. 2008; 5:20.Tavakoli N.P., Tobin E.H., Wong S.J., Dupuis A.P., Glasheen B., Kramer L.D., Bernard K.A. Identification of dengue virus in respiratory specimens from a patient who had recently traveled from a region where Dengue Virus infection is epidemic. J. Clin. Mirobiol. 2007; 5:1523–27.Tavakoli N.P., Tobin E.H., Wong S.J., Dupuis A.P., Glasheen B., Kramer L.D., Bernard K.A. Identification of dengue virus in respiratory specimens from a patient who had recently traveled from a region where Dengue Virus infection is epidemic. J. Clin. Mirobiol. 2007; 5:1523–27.Van der Schaar H.M., Rust M.J., Waarts B.-L., Van der Ende-Metselaar H., Kuhn R.J., Wilschut J., Zhuang X., Smit J.M. Characterization of the early events in dengue virus cell entry by biochemical assays and single virus tracking. J. Virol. 2007; 81(21):12019–28.Van der Schaar H.M., Rust M.J., Waarts B.-L., Van der Ende-Metselaar H., Kuhn R.J., Wilschut J., Zhuang X., Smit J.M. Characterization of the early events in dengue virus cell entry by biochemical assays and single virus tracking. J. Virol. 2007; 81(21):12019–28.WHO. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control. Geneva, Switzerland; 2009. 160 p.WHO. Dengue: Guidelines for Diagnosis, Treatment, Prevention and Control. Geneva, Switzerland; 2009. 160 p.WHO. Dengue haemorrhagic fever: Diagnosis, treatment, prevention and control. Geneva, Switzerland; 1997. 86 p.WHO. Dengue haemorrhagic fever: Diagnosis, treatment, prevention and control. Geneva, Switzerland; 1997. 86 p.Wu S.-J. L., Lee E.M., Putvatana R., Shurtliff R.N., Porter K.R., Suharyono W., Watts D.M., King C.C., Murphy G.S., Hayes C.G., Romano J.W. Detection of Dengue Viral RNA Using a Nucleic Acid Sequence-Based Amplification Assay. J. Clin. Mirobiol. 2001; 8:2794–98.Wu S.-J. L., Lee E. M., Putvatana R., Shurtliff R.N., Porter K.R., Suharyono W., Watts D.M., King C.C., Murphy G.S., Hayes C.G., Romano J.W. Detection of Dengue Viral RNA Using a Nucleic Acid Sequence-Based Amplification Assay. J. Clin. Mirobiol. 2001; 8:2794–98.Yong Y.K., Thayan R., Chong H.T. Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR. Singapore Med. J. 2007; 48:662–8.Yong Y.K., Thayan R., Chong H.T. Rapid detection and serotyping of dengue virus by multiplex RT-PCR and real-time SYBR green RT-PCR. Singapore Med. J. 2007; 48:662–8.Zandi K., Lani R., Wong P.F., Teoh B.T., Sam S.S., Johari J., Mustafa M.R., AbuBakar S. Flavone enhances dengue virus type-2 (NGC strain) infectivity and replication in vero cells. Molecules. 2012; 17(3):2437–45.Zandi K., Lani R., Wong P.F., Teoh B.T., Sam S.S., Johari J., Mustafa M.R., AbuBakar S. Flavone enhances dengue virus type-2 (NGC strain) infectivity and replication in vero cells. Molecules. 2012; 17(3):2437–45.Zompi S., Harris E. Animal Models of Dengue Virus Infection. Viruses. 2012; 4(1):62–82.Zompi S., Harris E. Animal Models of Dengue Virus Infection. Viruses. 2012; 4(1):62–82.

The authors declare that there are no conflicts of interest present.