<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">microbe</journal-id><journal-title-group><journal-title xml:lang="ru">Проблемы особо опасных инфекций</journal-title><trans-title-group xml:lang="en"><trans-title>Problems of Particularly Dangerous Infections</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0370-1069</issn><issn pub-type="epub">2658-719X</issn><publisher><publisher-name>Russian Research Anti-Plague Institute “Microbe”</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21055/0370-1069-2012-4-36-38</article-id><article-id custom-type="elpub" pub-id-type="custom">microbe-868</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>МИКРОБИОЛОГИЯ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>MICROBIOLOGY</subject></subj-group></article-categories><title-group><article-title>Исследование ультраструктуры двух фенотипически различных клонов холерного вибриона методами трансмиссионной и сканирующей электронной микроскопии</article-title><trans-title-group xml:lang="en"><trans-title>Examination of the Ultrastructure of Two Phenotypically Different Clones of 
                  V. cholerae by Means of Transmission and Scanning Electron Microscopy</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Коннов</surname><given-names>Н. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Konnov</surname><given-names>N. P.</given-names></name></name-alternatives><email xlink:type="simple">rusrapi@microbe.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Заднова</surname><given-names>С. П.</given-names></name><name name-style="western" xml:lang="en"><surname>Zadnova</surname><given-names>S. P.</given-names></name></name-alternatives><email xlink:type="simple">rusrapi@microbe.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кузнецов</surname><given-names>О. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Kuznetsov</surname><given-names>O. S.</given-names></name></name-alternatives><email xlink:type="simple">rusrapi@microbe.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Российский научно-исследовательский противочумный институт «Микроб»</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Russian Research Anti-Plague Institute “Microbe”</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2012</year></pub-date><pub-date pub-type="epub"><day>20</day><month>08</month><year>2012</year></pub-date><volume>0</volume><issue>4(114)</issue><fpage>36</fpage><lpage>38</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Коннов Н.П., Заднова С.П., Кузнецов О.С., 2012</copyright-statement><copyright-year>2012</copyright-year><copyright-holder xml:lang="ru">Коннов Н.П., Заднова С.П., Кузнецов О.С.</copyright-holder><copyright-holder xml:lang="en">Konnov N.P., Zadnova S.P., Kuznetsov O.S.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://journal.microbe.ru/jour/article/view/868">https://journal.microbe.ru/jour/article/view/868</self-uri><abstract><p>Представлены данные по изучению ультраструктуры двух фенотипически различных клонов (токсигенного и нетоксигенного) штамма Vibrio cholerae Дакка 35 методами электронной микроскопии. На поверхности бактерий у нетоксигенного клона обнаружен дополнительный слой (капсула) полисахаридной природы (отсутствующий у токсигенного), который способствовал изменению морфологии колоний.</p></abstract><trans-abstract xml:lang="en"><p>Submitted are the data of examination of the ultrastructure of two phenotypically different clones (toxigenic and non-toxigenic) of V. cholerae Dakka35 strain by means of electron microscopy. Additional layer (capsule) of polysaccharide nature is identified on the surface of non-toxigenic clone bacteria. This layer (absent in toxigenic clone) alters colony morphology.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Vibrio cholerae</kwd><kwd>токсигенные и нетоксигенные клетки</kwd><kwd>экзополисахаридный слой</kwd><kwd>трансмиссионная и сканирующая электронная микроскопия</kwd><kwd>toxigenic and non-toxigenic cells</kwd><kwd>exopolysaccharide layer</kwd><kwd>transmission and scanning electron microscopy</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Заднова С.П., Топорков А.В., Исаев Н.Д. Фенотипический анализ гетерогенного штамма Vibrio cholerae Дакка 35 Огава. Журн. микробиол., эпидемиол. и иммунобиол. 2004; 3:86–8.</mixed-citation><mixed-citation xml:lang="en">Zadnova S.P., Toporkov A.V., Isaev N.D. [Phenotypical analy- sis of heterogenic Vibrio cholerae strain Dacca 35 Ogawa]. Zh. Mikrobiol. Epidemiol. Immunobiol. 2004; 3:86–8.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Исаев Н.Д., Лозовский Ю.В., Заднова С.П., Смирнова Н.И. Популяционная неоднородность природных штаммов Vibrio cholerae классического биовара: координированное изменение морфологии колоний, подвижности, токсигенности и ферментативных свойств. Пробл. особо опасных инф. 2005; 1(89):43–6.</mixed-citation><mixed-citation xml:lang="en">Isaev N.D., Lozovsky Yu.V., Zadnova S.P., Smirnova N.I. [Populational heterogeneity of natural Vibrio cholerae strains of classical biovariant: coor- dinated changes in colony, morphology, motility, toxigenicity, and enzymatic characteristics]. Probl. Osobo Opasn. Infek. 2005; (89):43–6</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Кутырев В.В., Коннов Н.П., Волков Ю.П. Возбудитель чумы: ультраструктура и локализация в переносчике. М.: Медицина; 2007. 224 с.</mixed-citation><mixed-citation xml:lang="en">Kutyrev V.V., Konnov N.P., Volkov Yu.P. [The Causative Agent of Plague: Ultrastructure and Localization in a Vector]. M.: Meditsina; 2007. 224 p.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Ali A., Rashid M.H., Karaolis D.K. High-frequency rugose exopolysaccharide production by Vibrio cholerae. Appl. Environ. Microbiol. 2002; 68(1):5773–8.</mixed-citation><mixed-citation xml:lang="en">Ali A., Rashid M.H., Karaolis D.K. High-frequency rugose exopolysaccharide production by Vibrio cholerae. Appl. Environ. Microbiol. 2002; 68(1):5773–8.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Beyhan S., Yildiz F.H. Smooth to rugose phase variation in Vibrio cholerae can be mediated by a single nucleotide change that targets c-di-GMP signalling pathway. Mol. Microbiol. 2007; 63(4):995–1007.</mixed-citation><mixed-citation xml:lang="en">Beyhan S., Yildiz F.H. Smooth to rugose phase variation in Vibrio cholerae can be mediated by a single nucleotide change that targets c-di-GMP signalling pathway. Mol. Microbiol. 2007; 63(4):995–1007.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Faruque S.M., Nair G.B. Vibrio cholerae genomics and molecular biology. Norfolk, UK: Caister Academic Press, 2008. 218 p.</mixed-citation><mixed-citation xml:lang="en">Faruque S.M., Nair G.B. Vibrio cholerae genomics and molecular biology. Norfolk, UK: Caister Academic Press, 2008. 218 p.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Finkelstein R.A., Boesman-Finkelstein M., Sengupta D.K., Page W.J., Stranley C.M., Phillips T.E. Colonial opacity variations among the choleragenic vibrios Microbiology. 1997; 143(1):23–34.</mixed-citation><mixed-citation xml:lang="en">Finkelstein R.A., Boesman-Finkelstein M., Sengupta D.K., Page W.J., Stranley C.M., Phillips T.E. Colonial opacity variations among the choleragenic vibrios Microbiology. 1997; 143(1):23–34.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Johnson J.A., Panigrahi P., Morris J.G. Non-O1 Vibrio cholerae NRT36S produces a polysaccharide capsule that determines colony morphology, serum resistance, and virulence in mice. Infect. Immun. 1992; 60(3):864–9.</mixed-citation><mixed-citation xml:lang="en">Johnson J.A., Panigrahi P., Morris J.G. Non-O1 Vibrio cholerae NRT36S produces a polysaccharide capsule that determines colony morphology, serum resistance, and virulence in mice. Infect. Immun. 1992; 60(3):864–9.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Yildiz F.H., Schooinik G.K. Proc. Natl. Acad. Sci. USA. 1999; 96(7):4028–33.</mixed-citation><mixed-citation xml:lang="en">Yildiz F.H., Schooinik G.K. Proc. Natl. Acad. Sci. USA. 1999; 96(7):4028–33.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Yildiz F.H., Dolganov N.A., Schooinik G.K. VpsR, a member of the response regulators of the two-component regulatory systems, is required for expression of vps biosynthesis genes and EPS(ETr)-associated phenotypes in Vibrio cholerae O1 El Tor. J. Bacteriol. 2001; 183(5):1716–26.</mixed-citation><mixed-citation xml:lang="en">Yildiz F.H., Dolganov N.A., Schooinik G.K. VpsR, a member of the response regulators of the two-component regulatory systems, is required for expression of vps biosynthesis genes and EPS(ETr)-associated phenotypes in Vibrio cholerae O1 El Tor. J. Bacteriol. 2001; 183(5):1716–26.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Yildiz F.H., Lie X.S., Heydorn A., Schooinik G.K. Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant. Mol. Microbiol. 2004; 53(2):497–515.</mixed-citation><mixed-citation xml:lang="en">Yildiz F.H., Lie X.S., Heydorn A., Schooinik G.K. Molecular analysis of rugosity in a Vibrio cholerae O1 El Tor phase variant. Mol. Microbiol. 2004; 53(2):497–515.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
