Proteins of the plague microbe vaccine strain (Yersinia pestis EV NIIEG) with potential allergen properties

Abstract

Objective of the study was the search for proteins of the vaccine strain Yersinia pestis EV NIIEG with potential properties of allergens. Materials and methods. 3256 genomic proteins of Yersinia pestis EV NIIEG strain taken from the GenBank database were analyzed. Protein allergenicity was determined using computer information software. The Allpred program, integrated into the Protein Structure Discovery system, for predicting proteins as allergens, uses not only the primary sequence of amino acids, but also their spatial structure, as well as the physicochemical properties of amino acids. For all potential allergens found, their intracellular localization was additionally determined using the CELLO v.2.5 program: subCELlular Localization predictor and similarity to known human allergens in the AllergenOnline program. Results and discussion. Computer analysis of 3256 proteins revealed 170 (5.22 %) potential allergenic proteins of the vaccine strain Y. pestis EV NIIEG. Of these, 53 (31.18 %) relate to proteins with an unknown function (hypothetical protein), and 16 (9.41 %) relate to membrane proteins (membrane protein). The highest indicator of protein attribution to allergen was 0.824568569477881, and was observed in protein EXU71465.1 (autotransporter). Similarities to known allergens were found in 12 (7.06 %) of the predicted allergenic proteins. Of these, 4 (2.35 %) proteins have analogues with plant allergens, 5 (2.94 %) - with allergens from the animal kingdom, and 3 (1.76 %) - with yeast and mold allergens. The most promising when creating new hypoallergenic vaccines and drugs for diagnosing intensity of immunity in vaccinated individuals the allergen proteins belonging to the group of extracellular ones and outer membrane proteins have been identified. In Yersinia pestis EV NIIEG vaccine strain 38 of these or 22.35 % have been detected.