Efficiency of Using the Hydrogen Peroxide Vapor Generator “Fhileas 75” for Disinfection of the Air Ducts of separately Ventilated system for Infected Animal Housing

The aim of the work was to evaluate the efficiency of using the “Fhileas 75” hydrogen peroxide vapor generator for decontaminating the air ducts of the individually ventilated system, “Bio A.S.”, for housing of infected animals.

Materials and methods. The hydrogen peroxide vapor generator “Fhileas 75” (France), a disinfectant manufactured by “FHILEASAFE” (7 % hydrogen peroxide solution and 0.15 % peracetic acid solution), separately ventilated system “Bio A.S.” (Germany) for the infected animal housing were applied in the work. Serratia marcescens 9 was used as test-culture.

Results and discussion. The efficiency of using the hydrogen peroxide vapor generator “Fhileas 75” for decontamination of air ducts and internal surfaces of the rack of the individually ventilated system “Bio A.S.” on the test-culture S. marcescens 9 at 1·10⁶ mc/ml concentration has been established (operation parameters of the individually ventilated system unit are as follows: air exchange rate – 60 changes per hour, air flow volume – 28 m³/hour, number of disinfection cycles – 5, disinfectant spraying time – 97 min, exposure time – 24 hours).

1. Tyurin E.A., Khramov M.V., Dyatlov I.A. [Analysis of implementation of the requirements for provision of biological safety at a potentially hazardous facility]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2018; (2):95–100. DOI: 10.21055/0370-1069-2018-2-95-100.

2. Lyapin M.N. [Concerning hazard assessment technologies when working with pathogenic biological agents]. Infektsiya i Immunitet [Infection and Immunity]. 2017; (S):1059.

3. Malyukova T.A., Boiko A.V., PaninYu.A., Bezsmertny V.E., Kutyrev V.V. [The likelihood of realization of biorisks when working with PBA of I–II groups]. Epidemiologiya i Infektsionnye Bolezni [Epidemiology and Infectious Diseases]. 2016; 21(3):136–45. DOI: 10.18821/1560-9529-2016-21-3-136-145.

4. Boles K.S., Kannan K., Gill J., Felderman M., Gouvis H., Hubby B., Kamrud K.I., Venter J.C., Gibson D.G. Digital-tobiological converter for on-demand production of biologics. Nat. Biotechnol. 2017; 35(7):672–5. DOI: 10.1038/nbt.3859.

5. Trashchenko D., Kovaleva M. [Individually ventilated cages – extra financial investments or optimal protection of personnel and laboratory animals?] Mezhdunarodny Vestnik Veterinarii [International Veterinary Bulletin]. 2014; (1):100–3.

6. Germanchuk V.G., Semakova A.P., Shavina N.Yu. [Ethical principles for handling laboratory animals in an experiment with pathogenic biological agents of the I–II groups]. Problemy Osobo Opasnykh Infektsii [Problems of Particularly Dangerous Infections]. 2018; (4):33–8. DOI: 10.21055/0370-1069-2018-4-33-38.

7. Bureev I.A., Kushnir A.T., Slivko I.A., Korotkov O.V. [The advanced aerosol techniques of sanitation during manufacture of biological preparations]. Veterinariya [Veterinary Medicine]. 2015; (9):41–4.

8. Sisin E.I. [Aerosol disinfection]. Sanepidemcontrol’ [Sanitary and Epidemiological Control]. 2016; (2):84–7.

9. Kryuchkov A.V., Smirnov M.B., Bakulin V.M. [Technical means for carrying out aerosol disinfection of premises]. Dezinfektsionnoe Delo [Disinfection Affairs]. 2019; (1):5–11.

10. Alimov A.V., Zhuikov N.N., Vyatkina L.G., Rupysheva T.A. [Experience in using aerosol disinfection of ventilation systems]. Dezinfektsionnoe Delo [Disinfection Affairs]. 2019; (4):37–42. DOI: 10.35411/2076-457X-2019-4-37-42.