Preview

Problems of Particularly Dangerous Infections

Advanced search

Assessment of Resistance in Burkholderia pseudomallei to Benzalkonium Chloride and Antibiotics

https://doi.org/10.21055/0370-1069-2022-3-115-119

Abstract

The aim of the study was to investigate the features of resistance formation in Burkholderia pseudomallei to quaternary ammonium compounds, as well as to analyze its influence on the development of antibiotic resistance.

Materials and methods. 10 strains of melioidosis causative agent with typical cultural and morphological properties have been studied. The selection of variants resistant to benzalkonium chloride was carried out by successive passages on a dense nutrient medium with the addition of a disinfectant in increasing concentrations. The determination of sensitivity to benzalkonium chloride was performed through serial dilutions in agar, to antibacterial drugs from the groups of sulfonamides, β-lactams and tetracyclines – using disk diffusion method. Statistical processing of the obtained results was conducted with the help of the Microsoft Excel 2019 software. Arithmetic mean values and errors of mean values were calculated. The significance of differences between the parameters was determined applying Student’s t-test.

Results and discussion. All parental strains showed a similar degree of resistance to the disinfectant compound and most of the strains – susceptibility to the antibiotics tested. Cultivation of B. pseudomallei strains on a nutrient medium with the addition of benzalkonium chloride led to an increase in resistance to this disinfectant. In addition, an increase in the level of resistance to all studied antibiotics was found. Statistical processing of the data collected revealed a significant correlation between the change in sensitivity to benzalkonium chloride and the emergence of resistance to amoxicillin/ clavulanic acid and ceftazidime. It was found that the causative agent of melioidosis, with a natural high susceptibility to benzalkonium chloride, has a high potential for developing resistance to this disinfectant compound, which is of practical importance in the development of disinfection regimens using quarternary ammonium compounds. For the first time, a direct correlation between a decrease in the sensitivity to benzalkonium chloride in B. pseudomallei and emergence of resistance to amoxicillin/clavulonic acid and ceftazidime has been demonstrated.

About the Authors

D. N. Luchinin
Volgograd Research Anti-Plague Institute
Russian Federation

Dmitry N. Luchinin

7, Golubinskaya St., Volgograd, 400131



E. V. Molchanova
Volgograd Research Anti-Plague Institute
Russian Federation

7, Golubinskaya St., Volgograd, 400131



I. B. Zakharova
Volgograd Research Anti-Plague Institute
Russian Federation

7, Golubinskaya St., Volgograd, 400131



D. V. Viktorov
Volgograd Research Anti-Plague Institute
Russian Federation

7, Golubinskaya St., Volgograd, 400131



References

1. Zakharova I.B., Toporkov A.V., Viktorov D.V. [Melioidosis in aspects of epidemiology, clinical picture and laboratory dia¬ gnostics]. Infektsiya i Immunitet [Infection and Immunity]. 2021; 11(3):409–22. DOI: 10.15789/2220-7619-MIA-1584.

2. Chakravorty A., Heath C.H. Melioidosis: An updated review. Aust. J. Gen. Pract. 2019; 48(5):327–32. DOI: 10.31128/AJGP-0418-4558.

3. Chewapreecha C., Holden M.T., Vehkala M., Välimäki N., Yang Z., Harris S.R., Mather A.E., Tuanyok A., De Smet B., Le Hello S., Bizet C., Mayo M., Wuthiekanun V., Limmathurotsakul D., Phetsouvanh R., Spratt B.G., Corander J., Keim P., Dougan G., Dance D.A., Currie B.J., Parkhill J., Peacock S.J. Global and regional dissemination and evolution of Burkholderia pseudomallei. Nat. Microbiol. 2017; 2:16263. DOI: 10.1038/nmicrobiol.2016.263.

4. Limmathurotsakul D., Golding N., Dance D.A., Messina J.P., Pigott D.M., Moyes C.L., Rolim D.B., Bertherat E., Day N.P., Peacock S.J., Hay S.I. Predicted global distribution of Burkholderia pseudomallei and burden of melioidosis. Nat. Microbiol. 2016; 1(1):15008. DOI: 10.1038/nmicrobiol.2015.8.

5. Zakharova I.B., Toporkov A.V., Viktorov D.V. [Melioidosis and glanders: the current state of the problem and topical issues of epidemiological surveillance]. Zhurnal Mikrobiologii, Epidemiologii i Immunobiologii [Journal of Microbiology, Epidemiology and Immunobiology]. 2018; (6):103–9. DOI: 10.36233/0372-9311-20186-103-109.

6. Toporkov A.V., editor. [Melioidosis and Glanders]. Volgograd: Volga-Press; 2016. 400 p.

7. Ngauy V., Lemeshev Y., Sadkowski L., Crawford G. Cutaneous melioidosis in a man who was taken as a prisoner of war by the Japanese during World War II. J. Clin. Microbiol. 2005; 43(2):970–2. DOI: 10.1128/JCM.43.2.970-972.2005.

8. Berger S. Melioidosis and Glanders: Global Status. 2018 edition. GIDEON Informatics, Inc., Los Angeles, California, USA; 2018. 99 p.

9. Elschner M.C., Hnizdo J., Stamm I., El-Adawy H., Mertens K., Melzer F. Isolation of the highly pathogenic and zoonotic agent Burkholderia pseudomallei from a pet green Iguana in Prague, Czech Republic. BMC Vet. Res. 2014; 10:283. DOI: 10.1186/s12917-0140283-7.

10. Kanishchev V.V., Morozov A.S., Lakomov V.P., Omel’yanenko S.R., Bukhaeva Yu.V., Troshev R.G., Murav’ev A.G. [The use of disinfectants registered in Russia by the RCB protection forces in biological emergencies]. Vestnik Voisk Radiatsionnoi Khimicheskoi i Biologicheskoi Zashchity [Journal of RCB Protection Corps]. 2018; 2(4):57–67.

11. Health Council of the Netherlands. Resistance due to disinfectants. Background report to the advisory report Careful use of disinfectants. The Hague: Health Council of the Netherlands, 2016; publication No. A16/03E. [Internet]. Available from: https://www.healthcouncil.nl/binaries/healthcouncil/documenten/advisoryreports/2016/12/21/resistance-due-to-disinfectants-background-report/advisory-report-resistance-due-to-disinfectants.pdf.

12. Ahn Y., Kim J.M., Kweon O., Kim S.J., Jones R.C., Woodling K., Gamboa da Costa G., LiPuma J.J., Hussong D., Marasa B.S., Cerniglia C.E. Intrinsic resistance of Burkholderia cepacia complex to benzalkonium chloride. mBio. 2016; 7(6):e01716-16. DOI: 10.1128/mBio.01716-16.

13. Chacón-Jiménez L., Rojas-Jiménez K. Resistencia a desinfectantes y su relación con la resistencia a los antibióticos. Acta Médica Costarricense. 2020; 62(1):7–12.

14. Wuthiekanun V., Amornchai P., Saiprom N., Chantratita N., Chierakul W., Koh G.C., Chaowagul W., Day N.P., Limmathurotsakul D., Peacock S.J. Survey of antimicrobial resistance in clinical Burkholderia pseudomallei isolates over two decades in Northeast Thailand. Antimicrob. Agents Chemother. 2011; 55(11):5388–91. DOI: 10.1128/AAC.05517-11.

15. Khosravi Y., Vellasamy K.M., Mariappan V., Ng S.L., Vadivelu J. Antimicrobial susceptibility and genetic characterisation of Burkholderia pseudomallei isolated from Malaysian patients. ScientificWorldJournal. 2014; 2014:132971. DOI: 10.1155/2014/132971.

16. Sergevnin V.I., Klyukina T.V., Volkova E.O., Reshetnikova N.I., Klyuchareva N.M. [Formation of resistance to disinfectants in Enterobacter cloacae and Pseudomonas aerogenosa under the influence of bactericidal concentrations of drugs in the experiment]. Meditsinsky Al’manakh [Medical Almanac]. 2015; 5(40):112–5.

17. Shkarin V.V., Kovalishena O.V., Blagonravova A.S., Vorob’eva O.N., Alekseeva I.G., Yakovleva E.I., Bugrova M.L. Formation of bacterial resistance to quaternary ammonium compounds under experimental conditions. Meditsinsky Al’manakh [Medical Almanac]. 2012; 3(22):129–33.

18. Aarestrup F.M., Hasman H. Susceptibility of different bacterial species isolated from food animals to copper sulphate, zinc chloride and antimicrobial substances used for disinfection. Vet. Microbiol. 2004; 100(1–2):83–9. DOI: 10.1016/j.vetmic.2004.01.013.

19. Rose H., Baldwin A., Dowson C.G., Mahenthiralingam E. Biocide susceptibility of the Burkholderia cepacia complex. J. Antimicrob. Chemother. 2009; 63(3):502–10. DOI: 10.1093/jac/dkn540.

20. Jenney A.W., Lum G., Fisher D.A., Currie B.J. Antibiotic susceptibility of Burkholderia pseudomallei from tropical northern Australia and implications for therapy of melioidosis. Int. J. Antimicrob. Agents. 2001; 17(2):109–13. DOI: 10.1016/s0924-8579(00)00334-4.

21. Anutrakunchai C., Bolscher J., Krom B.P., Kanthawong S., Chareonsudjai S., Taweechaisupapong S. Impact of nutritional stress on drug susceptibility and biofilm structures of Burkholderia pseudomallei and Burkholderia thailandensis grown in static and microfluidic systems. PloS One. 2018; 13(3):e0194946. DOI: 10.1371/journal.pone.0194946.


Review

For citations:


Luchinin D.N., Molchanova E.V., Zakharova I.B., Viktorov D.V. Assessment of Resistance in Burkholderia pseudomallei to Benzalkonium Chloride and Antibiotics. Problems of Particularly Dangerous Infections. 2022;(3):115-119. (In Russ.) https://doi.org/10.21055/0370-1069-2022-3-115-119

Views: 566


Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.


ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)