Maintenance of Francisella tularensis 15 RIEH and Brucella abortus 19 BA Strains in a Viable State by Means of Deep Freezing
https://doi.org/10.21055/0370-1069-2015-1-63-66
Abstract
Assessed are the methods for storage of microorganisms in a viable state under various technological conditions for a period of two years. Compared is the survivability of the test-strains of tularemia and brucellosis agents (exemplified by Francisella tularensis 15 RIEH and Brucella abortus 19 BA) when stored on the nutrient media at temperatures raging 0 °C up +8 °C and at -70 °C for over a year. Made has been an estimate of survivability of the vaccine strains stored at -70 °C within two years term. It is determined that optimum media for storing the stated above microorganisms with no changes in their morphological characteristics at -70 °C are sucrose-gelatin agar and Albimi broth with 10 % glycerin. Demonstrated is the fact that it is more efficient to conserve microorganisms stored in working collections by way of deep freezing.
About the Authors
A. N. Malakhaeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. Yu. Lyashova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
O. P. Plotnikov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
A. V. Osin
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
Russian Federation
References
1. Bezsmertny V.E., Popov V.P., Zhukov V.I., Ivanova S.M. [Review of tularemia epizootic situation in the Russian Federation territory in the second half of 2008 and prognosis for the first half of 2009]. Probl. Osobo Opasn. Infek. 2009; 2(100):11–3.
2. [Diagnostic nutrient media control against biological parameters for agents of plague, cholera, anthrax, tularemia, brucellosis, and legionellosis]. MR 3.3.2.2124-06. M.; 2006. 29 p.
3. Kupletskaya M.B., Netrusov A.I. [Viability of lyophilized microorganisms after 50 years of storage]. Mikrobiologia. 2011; 80(6):842–6.
4. Lyamkin G.I., Tikhenko N.I., Manin E.A., Rusanova D.V., Golovneva S.I., Vilinskaya S.V., Kulichenko A.N. [Brucellosis epidemiological situation and its morbidity in the Russian Federation in 2011, and prognosis for 2012]. Probl. Osobo Opasn. Infek. 2012; 1(111):26–9.
5. Pokhilenko V.D., Baranov A.M., Detushev K.V. [Methods of longterm storage of microorganisms’ collection cultures and development trends]. Izvestiya VUZov. 2009; 4:99–121.
6. Gibson L.F., Khoury J.T. Storage and survival of bacteria by ultrafreeze. Lett. Appl. Microbiol. 1986; 3(6):127–9.
Review
For citations:
Malakhaeva A.N., Lyashova O.Yu., Plotnikov O.P., Osin A.V. Maintenance of Francisella tularensis 15 RIEH and Brucella abortus 19 BA Strains in a Viable State by Means of Deep Freezing. Problems of Particularly Dangerous Infections. 2015;(1):63-66. (In Russ.) https://doi.org/10.21055/0370-1069-2015-1-63-66
Views:
707
Email this article 