Phylogeny and Biological Properties of Brucella abortus Strains Isolated in the Volga and Central Federal Districts in 2017–2024
D. A. Kovalev,
S. V. Pisarenko,
I. V. Kuznetsova,
V. I. Kolomytseva,
D. G. Ponomarenko,
N. A. Shapakov,
A. A. Khachaturova,
N. S. Safonova https://doi.org/10.21055/0370-1069-2025-4-82-87
Abstract
The aim of the work was to study biological, molecular-genetic properties and to determin the phylogenetic affiliation of Brucella abortus strains isolated in the Volga and Central Federal Districts.
Materials and methods. A research of 21 strains of B. abortus isolated in the Volga Federal District and the Central Federal District in 2017–2024 was conducted. Genome sequencing was performed on the DNBSEQ G50RS platform (MGI, China) using the MGI Easy FAST FS DNA Library Prep Set V2.1 reagent kit (MGI, China) according to a standard protocol. Phylogenetic reconstruction was performed using the maximum likelihood method in the “RealPhy” software.
Results and discussion. All strains have tinctorial, morphological, and cultural properties characteristic of typical B. abortus strains. The topology of the phylogenetic tree indicates the common origin of the strains isolated in the Volga Federal District and the Central Federal District in 2017–2024. It has been found that the studied isolates are genetically close to the B. abortus strains isolated from biomaterial from animals with brucellosis and food products of animal origin from the Lipetsk Region (2017), Samara Region (2016), Stavropol Territory (2015), Republic of Dagestan (2015), Republic of Kalmykia (2012). The general clustering of strains from the Volga Federal District and the Central Federal District and from different entities of the North Caucasus Federal District and the Southern Federal District indicates the presence of a retrospective epidemiological link between brucellosis foci and the affected over the long period territory of the southern European part of Russia, including the North Caucasus.
About the Authors
D. A. Kovalev
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
Dmitry A. Kovalev,
13–15, Sovetskaya St., Stavropol, 355035
S. V. Pisarenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
13–15, Sovetskaya St., Stavropol, 355035
I. V. Kuznetsova
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
13–15, Sovetskaya St., Stavropol, 355035
V. I. Kolomytseva
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
13–15, Sovetskaya St., Stavropol, 355035
D. G. Ponomarenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
13–15, Sovetskaya St., Stavropol, 355035
N. A. Shapakov
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
13–15, Sovetskaya St., Stavropol, 355035
A. A. Khachaturova
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
13–15, Sovetskaya St., Stavropol, 355035
N. S. Safonova
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
13–15, Sovetskaya St., Stavropol, 355035
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For citations:
Kovalev D.A., Pisarenko S.V., Kuznetsova I.V., Kolomytseva V.I., Ponomarenko D.G., Shapakov N.A., Khachaturova A.A., Safonova N.S. Phylogeny and Biological Properties of Brucella abortus Strains Isolated in the Volga and Central Federal Districts in 2017–2024. Problems of Particularly Dangerous Infections. 2025;(4):82-87. (In Russ.) https://doi.org/10.21055/0370-1069-2025-4-82-87
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