Potential Immunodiagnostic Targets for Detection of Burkholderia pseudomallei
https://doi.org/10.21055/0370-1069-2026-1-26-33
Abstract
Melioidosis is a particularly dangerous infectious disease that affects humans and animals primarily in Southeast Asia and northern Australia. The predicted spread of the natural range of the pathogen, Burkholderia pseudomallei, and the increase in melioidosis cases in the coming years threaten to become a serious public health issue. Since it takes up to seven days to isolate and identify a B. pseudomallei culture, a reliable test for its rapid direct detection in clinical samples is needed, which will allow for the initiation of treatment with appropriate antibiotics, preventing relapses and reducing mortality. The development of diagnostic approaches is complicated by the significant adaptive plasticity of the B. pseudomallei genome, which acquires new coding sequences as a result of horizontal gene transfer from microorganisms that share a common ecological niche with the causative agent of melioidosis. Most of the developed immunodiagnostic tests for the detection of B. pseudomallei were created without proper standardization and are not commercially available. These experimental tests have insufficient sensitivity and specificity and work best with an isolated bacterial culture, minimizing the advantages of rapid diagnostics. The selection of a diagnostic target remains the main challenge in developing a simple, cost-effective and valid rapid immunodiagnostic test for the detection of B. pseudomallei. This review presents an analysis of the literature data on existing and promising methodological approaches to the rapid detection of the causative agent of melioidosis using immunological methods and the search for new potential antigen targets.
Supporting agencies: The authors declare no additional financial support for this study
About the Authors
Yu. A. Zhukova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
Yulia A. Zhukova
7, Golubinskaya St., Volgograd, 400066
I. B. Zakharova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
7, Golubinskaya St., Volgograd, 400066
References
1. Hall C.M., Baker A.L., Sahl J.W., Mayo M., Scholz H.C., Kaestli M., Schupp J., Martz M., Settles E.W., Busch J.D., SidakLoftis L., Thomas A., Kreutzer L., Georgi E., Schweizer H.P., Warner J.M., Keim Р., Currie B.J., Wagner D.M. Expanding the Burkholderia pseudomallei complex with the addition of two novel species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Appl. Environ. Microbiol. 2022; 88(1):e01583-21. DOI: 10.1128/AEM.01583-21.
2. Meumann E.M., Limmathurotsakul D., Dunachie S.J., Wiersinga W.J., Currie B.J. Burkholderia pseudomallei and melioidosis. Nat. Rev. Microbiol. 2024; 22(3):155–69. DOI: 10.1038/s41579-023-00972-5.
3. Oslan S.N.H., Yusoff A.H., Mazlan M., Lim S.J., Khoo J.J., Oslan, S.N., Ismail A. Comprehensive approaches for the detection of Burkholderia pseudomallei and diagnosis of melioidosis in human and environmental samples. Microb. Pathog. 2022; 169:105637. DOI: 10.1016/j.micpath.2022.105637.
4. Birnie E., Biemond J.J., Wiersinga W.J. Drivers of melioidosis endemicity: epidemiological transition, zoonosis, and climate change. Curr. Opin. Infect. Dis. 2022; 35(3):196–204. DOI: 10.1097/QCO.0000000000000827.
5. Currie B.J. Melioidosis and Burkholderia pseudomallei: progress in epidemiology, diagnosis, treatment and vaccination. Curr. Opin. Infect. Dis. 2022; 35(6):517–23. DOI: 10.1097/QCO.0000000000000869.
6. Norman F.F., Chen L.H. Travel-associated melioidosis: a narrative review. J. Travel. Med. 2023; 30(3):taad039. DOI: 10.1093/jtm/taad039.
7. Gassiep I., Armstrong M., Norton R. Human melioidosis. Clin. Microbiol. Rev. 2020; 33(2):e00006-19. DOI: 10.1128/CMR.00006-19.
8. Chantratita N., Phunpang R., Yarasai A., Dulsuk A., Yimthin T., Onofrey L.A., Coston T.D., Thiansukhon E., Chaisuksant S., Tanwisaid K., Chuananont S., Morakot C., Sangsa N., Chayangsu S., Silakun W., Buasi N., Chetchotisakd P., Day N.P., Lertmemongkolchai G., West T.E. Characteristics and one year outcomes of melioidosis patients in Northeastern Thailand: A prospective, multicenter cohort study. Lancet Reg. Health. Southeast Asia. 2023; 9:100118. DOI: 10.1016/j.lansea.2022.100118.
9. Sullivan R.P., Marshall C.S., Anstey N.M., Ward L., Currie B.J. 2020 Review and revision of the 2015 Darwin melioidosis treatment guideline; paradigm drift not shift. PLoS Negl. Trop. Dis. 2020; 14(9):e0008659. DOI: 10.1371/journal.pntd.0008659.
10. Wajanarogana S., Taylor W.R., Kritsiriwuthinan K. Enhanced serodiagnosis of melioidosis by indirect ELISA using the chimeric protein rGroEL-FLAG300 as an antigen. BMC Infect. Dis. 2022; 22(1):387. DOI: 10.1186/s12879-022-07369-4.
11. Selvam K., Khalid M.F., Mustaffa K.M.F., Harun A., Aziah I. BipD of Burkholderia pseudomallei: structure, functions, and detection methods. Microorganisms. 2021; 9(4):711. DOI: 10.3390/microorganisms9040711.
12. Chaichana P., Jenjaroen K., Amornchai P., Chumseng S., Langla S., Rongkard P., Sumonwiriya M., Jeeyapant A., Chantratita N., Teparrukkul P., Limmathurotsakul D., Day N.P.J., Wuthiekanun V., Dunachie S.J. Antibodies in melioidosis: the role of the indirect hemagglutination assay in evaluating patients and exposed populations. Am. J. Trop. Med. Hyg. 2018; 99(6):1378–85. DOI: 10.4269/ajtmh.17-0998.
13. Lau S.K., Sridhar S., Ho C.C., Chow W.N., Lee K.C., Lam C.W., Yuen K.Y., Woo P.C. Laboratory diagnosis of melioidosis: past, present and future. Exp. Biol. Med. (Maywood). 2015; 240(6):742–51. DOI: 10.1177/1535370215583801.
14. Prokhvatilova E.V., Antonov V.A., Viktorov D.V., Khrapova N.P., Tkachenko G.A., Ilyukhin V.I., Zakharova I.B., Grishina M.A., Plekhanova N.G., Novitskaya I.V., Kulakov M.Ya., Bulatova T.V., Korsakova I.I., Savtchenko S.S., Bondareva O.S., Teteryatnikova N.N., Senina T.V., Lopasteiskaya Ya.A., Baturin A.A., Kulikova A.S. [The comparative evaluation of informativeness of immunologic and molecular-genetic methods and means during stages of specific indication of melioidosis agent]. Klinicheskaya Laboratornaya Diagnostika [Russian Clinical Laboratory Diagnostics]. 2014; 59(12):55–9.
15. Bzdyl N.M., Moran C.L., Bendo J., Sarkar-Tyson M. Pathogenicity and virulence of Burkholderia pseudomallei. Virulence. 2022; 13(1):1945–65. DOI: 10.1080/21505594.2022.2139063.
16. Holden M.T., Titball R.W., Peacock S.J., Cerdeño-Tárraga A.M., Atkins T., Crossman L.C., Pitt T., Churcher C., Mungall K., Bentley S.D., Sebaihia M., Thomson N.R., Bason N., Beacham I.R., Brooks K., Brown K.A., Brown N.F., Challis G.L., Cherevach I., Chillingworth T., Cronin A., Crossett B., Davis P., DeShazer D., Feltwell T., Fraser A., Hance Z., Hauser H., Holroyd S., Jagels K., Keith K.E., Maddison M., Moule S., Price C., Quail M.A., Rabbinowitsch E., Rutherford K., Sanders M., Simmonds M., Songsivilai S., Stevens K., Tumapa S., Vesaratchavest M., Whitehead S., Yeats C., Barrell B.G., Oyston P.C.F., Parkhill J. Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc. Natl Acad. Sci. USA. 2004; 101(39):14240–5. DOI: 10.1073/pnas.0403302101.
17. Webb J.R., Rachlin A., Rigas V., Sarovich D.S., Price E.P., Kaestli M., Mayo M., Currie B.J. Tracing the environmental footprint of the Burkholderia pseudomallei lipopolysaccharide genotypes in the tropical “Top End” of the Northern Territory, Australia. PLoS Negl. Trop. Dis. 2019; 13(7):e0007369. DOI: 10.1371/journal.pntd.0007369.
18. Shaw T., Tellapragada C., Kamath A., Kalwaje Eshwara V., Mukhopadhyay C. Implications of environmental and pathogenspecific determinants on clinical presentations and disease outcome in melioidosis patients. PLoS Negl. Trop. Dis. 2019; 13(5):e0007312. DOI: 10.1371/journal.pntd.0007312.
19. Chomkatekaew C., Boonklang P., Sangphukieo A., Chewapreecha C. An evolutionary arms race between Burkholderia pseudomallei and host immune system: what do we know? Front. Microbiol. 2021; 11:612568. DOI: 10.3389/fmicb.2020.612568.
20. Dulsuk A., Paksanont S., Sangchankoom A., Ekchariyawat P., Phunpang R., Jutrakul Y., Chantratita N., West T.E. Validation of a monoclonal antibody-based immunofluorescent assay to detect Burkholderia pseudomallei in blood cultures. Trans. R. Soc. Trop. Med. Hyg. 2016; 110(11):670–2. DOI: 10.1093/trstmh/trw079.
21. Woods K.L., Boutthasavong L., NicFhogartaigh C., Lee S.J., Davong V., AuCoin D.P., Dance D.A.B. Evaluation of a rapid diagnostic test for detection of Burkholderia pseudomallei in the Lao People’s Democratic Republic. J. Clin. Microbiol. 2018; 56(7):e02002-17. DOI: 10.1128/JCM.02002-17.
22. Dharakul T., Songsivilai S., Smithikarn S., Thepthai C., Leelaporn A. Rapid identification of Burkholderia pseudomallei in blood cultures by latex agglutination using lipopolysaccharide-specific monoclonal antibody. Am. J. Trop. Med. Hyg. 1999; 61(4):658–62. DOI: 10.4269/ajtmh.1999.61.658.
23. Anuntagool N., Naigowit P., Petkanchanapong V., Aramsri P., Panichakul T., Sirisinha S. Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J. Med. Microbiol. 2000; 49(12):1075–8. DOI: 10.1099/0022-1317-49-12-1075.
24. Hodgson K., Engler C., Govan B., Ketheesan N., Norton R. Comparison of routine bench and molecular diagnostic methods in identification of Burkholderia pseudomallei. J. Clin. Microbiol. 2009; 47(5):1578–80. DOI: 10.1128/JCM.02507-08.
25. Duval B.D., Elrod M.G., Gee J.E., Chantratita N., Tandhavanant S., Limmathurotsakul D., Hoffmaster A.R. Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei. Am. J. Trop. Med. Hyg. 2014; 90(6):1043–6. DOI: 10.4269/ajtmh.14-0025.
26. Muangsombut V., Withatanung P., Chantratita N., Chareonsudjai S., Lim J., Galyov E.E., Ottiwet O., Sengyee S., Janesomboon S., Loessner M.J., Dunne M., Korbsrisate S. Rapid clinical screening of Burkholderia pseudomallei colonies by a bacteriophage tail fiber-based latex agglutination assay. Appl. Environ. Microbiol. 2021; 87(12):e0301920. DOI: 10.1128/AEM.03019-20.
27. Peeters M., Chung P., Lin H., Mortelmans K., Phe C., San C., Kuijpers L.M.F., Teav S., Phe T., Jacobs J. Diagnostic accuracy of the InBiOS AMD rapid diagnostic test for the detection of Burkholderia pseudomallei antigen in grown blood culture broth. Eur. J. Clin. Microbiol. Infect. Dis. 2018; 37(6):1169–77. DOI: 10.1007/s10096-018-3237-3.
28. Rizzi M.C., Rattanavong S., Bouthasavong L., Seubsanith A., Vongsouvath M., Davong V., De Silvestri A., Manciulli T., Newton P.N., Dance D.A. Evaluation of the Active Melioidosis Detect™ test as a point-of-care tool for the early diagnosis of melioidosis: a comparison with culture in Laos. Trans. R. Soc. Trop. Med. Hyg. 2019; 113(12):757–63. DOI: 10.1093/trstmh/trz092.
29. Currie B.J., Woerle C., Mayo M., Meumann E.M., Baird R.W. What is the role of lateral flow immunoassay for the diagnosis of melioidosis? Open Forum Infect. Dis. 2022; 9(5):ofac149. DOI: 10.1093/ofid/ofac149.
30. Houghton R.L., Reed D.E., Hubbard M.A., Dillon M.J., Chen H., Currie B.J., Mayo M., Sarovich D.S., Theobald V., Limmathurotsakul D., Wongsuvan G., Chantratita N., Peacock S.J., Hoffmaster A.R., Duval B., Brett P.J., Burtnick M.N., Aucoin D.P. Development of a prototype lateral flow immunoassay (LFI) for the rapid diagnosis of melioidosis. PLoS Negl. Trop. Dis. 2014; 8(3):e2727. DOI: 10.1371/journal.pntd.0002727.
31. Venkateswaran K.S., Parameswaran N., Sarwar J., Plummer A., Santos A., Pillai C.A., Bowen S., Granville M., Selvan S., Babu P., Thirunavukkarasu N., Venkateswaran N., Sharma S., Morse S.A., Anderson K., Hodge D.R., Pillai S.P. Rapid presumptive identification of Burkholderia mallei and Burkholderia pseudomallei clinical isolates using a highly specific lateral flow assay. Health Secur. 2022; 20(2):164–71. DOI: 10.1089/hs.2021.0172.
32. Rongkard P., Hantrakun V., Dittrich S., Srilohasin P., Amornchai P., Langla S., Lim C., Day N.P.J., AuCoin D., Wuthiekanun V., Limmathurotsakul D. Utility of a lateral flow immunoassay (LFI) to detect Burkholderia pseudomallei in soil samples. PLoS Negl. Trop. Dis. 2016; 10(12):e0005204. DOI: 10.1371/journal.pntd.0005204.
33. Nualnoi T., Wongwitwichot P., Kaewmanee S., Chanchay P., Wongpanti N., Ueangsuwan T., Siangsanor R., Chotirouangnapa W., Saechin T., Thungtin S., Szekely J., Wattanachant C., Saechan V. Development of an antigen capture lateral flow immunoassay for the detection of Burkholderia pseudomallei. Diagnostics. 2024; 14(10):1033. DOI: 10.3390/diagnostics14101033.
34. Pumpuang A., Dunachie S.J., Phokrai P., Jenjaroen K., Sintiprungrat K., Boonsilp S., Brett P.J., Burtnick M.N., Chantratita N. Comparison of O-polysaccharide and hemolysin co-regulated protein as target antigens for serodiagnosis of melioidosis. PLoS Negl. Trop. Dis. 2017; 11(3):e0005499. DOI: 10.1371/journal.pntd.0005499.
35. Amornchai P., Hantrakun V., Wongsuvan G., Wuthiekanun V., Wongratanacheewin S., Teparrakkul P., West T.E., AuCoin D., Day N.P.J., Brett P.J., Burtnick M.N., Chantratita N., Limmathurotsakul D. Evaluation of antigen-detecting and antibody-detecting diagnostic test combinations for diagnosing melioidosis. PLoS Negl. Trop. Dis. 2021; 15(11):e0009840. DOI: 10.1371/journal.pntd.0009840.
36. Kritsiriwuthinan K., Wajanarogana S., Choosang K., Homsian J., Rerkthanom S. Production and evaluation of recombinant Burkholderia pseudomallei GroEL and OmpA proteins for serodiagnosis of melioidosis. Acta Trop. 2018; 178:333–9. DOI: 10.1016/j.actatropica.2017.10.019.
37. Lee S.H., Lu Y.P., Shih W.L., Chang C.D., Tu Y.C., Lai I.H. Development of an immunoassay using recombinant outer membrane protein A and flagellin for diagnosis of goats with melioidosis. J. Vet. Med. Sci. 2020; 82(3):325–32. DOI: 10.1292/jvms.19-0072.
38. Kuzyutina Yu.A., Zakharova I.B., Savchenko S.S., Lopasteyskaya Ya.A., Molchanova E.V., Viktorov D.V. [Search for potential targets for the detection and differentiation of the causative agents of melioidosis and glanders strains]. Vestnik Volgogradskogo Gosudarstvennogo Meditsinskogo Universiteta[Bulletin of Volgograd State Medical University]. 2016; (4):114–7.
39. Kuzyutina Yu.A., Zakharova I.B., Viktorov D.V. [Engineering E. coli recombinant strains for high yield production of Burkholderia pseudomallei specific antigens]. Infektsiya i Immunitet [Russian Journal of Infection and Immunity]. 2019; 9(1):203–8. DOI: 10.15789/2220-7619-2019-1-203-208.
40. Koosakulnirand S., Phokrai P., Jenjaroen K., Roberts R.A., Utaisincharoen P., Dunachie S.J., Brett P.J., Burtnick M.N., Chantratita N. Immune response to recombinant Burkholderia pseudomallei FliC. PLoS One. 2018; 13(6):e0198906. DOI: 10.1371/journal.pone.0198906.
41. Capelli R., Peri C., Villa R., Nithichanon A., Conchillo-Solé O., Yero D., Gagni P., Chiari M., Lertmemongkolchai G., Cretich M., Daura X., Bolognesi M., Colombo G., Gourlay L.J. BPSL1626: reverse and structural vaccinology reveal a novel candidate for vaccine design against Burkholderia pseudomallei. Antibodies (Basel). 2018; 7(3):26. DOI: 10.3390/antib7030026.
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For citations:
Zhukova Yu.A., Zakharova I.B. Potential Immunodiagnostic Targets for Detection of Burkholderia pseudomallei. Problems of Particularly Dangerous Infections. 2026;(1):26-33. (In Russ.) https://doi.org/10.21055/0370-1069-2026-1-26-33
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