Analysis of Vibrio cholerae Genotype Stability at Low Temperature and Nutrients Deficiency
https://doi.org/10.21055/0370-1069-2016-3-52-56
Abstract
Objective is an experimental study of the low temperature and nutrient deficiency effects on the stability of Vibrio cholerae El Tor genetic loci complex.
Materials and methods. The stability of the genetic loci (i.e. the basic pathogenicity genes; genome fragments containing variable tandem repeats and sites of rare cutting restrictase recognition defining VNTR- and PFGE-profiles of the isolates, accordingly; V. cholerae «housekeeping» genes) of the four Vibrio cholerаe El Tor strains was investigated on the experimental microcosm at different stages of incubation.
Results and conclusions. The study demonstrated that clones with altered VNTR- and PFGE-profiles (the detection frequency was 4–8·10–2) were being formed when strains were cultivated under nutrient deficiency and low temperature conditions. Neither changes in the structure of «housekeeping» genes nor loss of pathogenicity genes of the mobile genetic elements were revealed. The findings affirm the probability of analyzed V. cholerae loci alteration occurrence under unfavorable environment conditions. Obtained results should be taken into consideration for interpretation of molecular typing data.
About the Authors
L. V. MironovaRussian Federation
78, Trilissera St., Irkutsk, 664047, Russian Federation
Zh. Yu. Khunkheeva
Russian Federation
78, Trilissera St., Irkutsk, 664047, Russian Federation
E. A. Basov
Russian Federation
78, Trilissera St., Irkutsk, 664047, Russian Federation
A. S. Ponomareva
Russian Federation
78, Trilissera St., Irkutsk, 664047, Russian Federation
S. K. Mitkeeva
Russian Federation
78, Trilissera St., Irkutsk, 664047, Russian Federation
S. V. Balakhonov
Russian Federation
78, Trilissera St., Irkutsk, 664047, Russian Federation
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Review
For citations:
Mironova L.V., Khunkheeva Zh.Yu., Basov E.A., Ponomareva A.S., Mitkeeva S.K., Balakhonov S.V. Analysis of Vibrio cholerae Genotype Stability at Low Temperature and Nutrients Deficiency. Problems of Particularly Dangerous Infections. 2016;(3):52-56. (In Russ.) https://doi.org/10.21055/0370-1069-2016-3-52-56