THE ROLE OF SMALL RNAS IN CONTROLLING EXPRESSION OF GENES INVOLVED IN THE IMPLEMENTATION OF VIBRIO CHOLERAE PATHOGENICITY
https://doi.org/10.21055/0370-1069-2017-2-36-39
Abstract
Currently, bacterial small RNAs with regulatory functions are characterized as a heterogeneous claster of highly structured single-stranded RNAs which usually would not be translated into proteins. A large group of small RNAs consists of small regulatory RNAs functioning through the mechanism of complementary interaction between small regulatory RNA bases and bacterial messenger RNAs (mRNA). The review provides the description of direct participation of small RNA mechanisms in realization of pathogenic properties, biofilm formation and virulence regulation in V. cholerae strains. In particular, the Hfq-dependent small RNAs that control the expression of genes responsible for virulence and biofilm formation of V. choleraе, and the impact of small RNA on the secretion system of VI type are presented. We also characterized the small RNAs–controlled process of V. cholerae vesicles formation, which have a great significance for colonization ability of cholera vibrios. In addition, such method of regulation at RNA level as “riboswitches”, (RNA switches) is described.
About the Authors
R. V. Pisanov
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
117/40, M.Gor`kogo St., Rostov-on-Don, 344002
Russian Federation
117/40, M.Gor`kogo St., Rostov-on-Don, 344002
A. S. Vodop’yanov
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
117/40, M.Gor`kogo St., Rostov-on-Don, 344002
Russian Federation
117/40, M.Gor`kogo St., Rostov-on-Don, 344002
D. I. Simakova
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
117/40, M.Gor`kogo St., Rostov-on-Don, 344002
Russian Federation
117/40, M.Gor`kogo St., Rostov-on-Don, 344002
References
1. Arraiano C.M., Fialho A.M. O Mundo do RNA: Novos Desafios e Perspectivas Futuras. Lidel: Lisboa-Porto; 2007.
2. Bardill J.P., Hammer B.K. Non-coding sRNAs regulate virulence in the bacterial pathogen Vibrio cholerae. RNA biol. 2012; 9(4):392–401. DOI: 10.4161/rna.19975.
3. Bardill J.P., Zhao X., Hammer B.K. The Vibrio cholerae quorum sensing response is mediated by Hfq-dependent sRNA/ mRNA base pairing interactions. Mol. Microbiol. 2011; 80(5):1381– 94. DOI: 10.1111/j.1365-2958.2011.07655.x.
4. Bastet L., Dube A., Masse E., Lafontaine D.A. New insights into riboswitch regulation mechanisms. Mol. microbiol. 2011; 80:1148–54. DOI: 10.1111/j.1365-2958.2011.07654.x.
5. Bejerano-Sagie M., Xavier K.B. The role of small RNAs in quorum sensing. Curr. Opin. Microbiol. 2007; 10(2):189–98. DOI: 10.1016/j.mib.2007.03.009.
6. Beyhan S., Yildiz F.H. Smooth to rugose phase variation in Vibrio cholerae can be mediated by a single nucleotide change that targets c-di-GMP signalling pathway. Mol. microbiol. 2007; 63(4):995–1007. DOI: 10.1111/j.1365-2958.2006.05568.x.
7. Chao Y., Vogel J. The role of Hfq in bacterial pathogens. Curr. Opin. Microbiol. 2010; 13(1):24–33. DOI: 10.1016/j.mib.2010.01.001.
8. Dalia A.B., Lazinski D.W., Camilli A. Identification of a membrane-bound transcriptional regulator that links chitin and natural competence in Vibrio cholerae. MBio. 2014; 5(1):1028–33. DOI: 10.1128/mBio.01028-13.
9. Hammer B.K., Bassler B.L. Regulatory small RNAs circumvent the conventional quorum sensing pathway in pandemic Vibrio cholerae. Proc. Nat. Acad. Sci. USA. 2007; 104(27):1145–9. DOI: 10.1073/pnas.0703860104.
10. Kariisa A.T., Weeks K., Tamayo R. The RNA Domain Vc1 Regulates Downstream Gene Expression in Response to Cyclic Diguanylate in Vibrio cholerae. PLoS One. 2016; 11(2):1484–8. DOI: 10.1371/journal.pone.0148478.
11. Kovacikova G., Lin W., Skorupski K. Vibrio cholerae AphA uses a novel mechanism for virulence gene activation that involves interaction with the LysR-type regulator AphB at the tcpPH promoter. Mol. Microbiol. 2004; 53(1):129–42. DOI: 10.1111/j.1365-2958.2004.04121.x.
12. Kovacikova G., Skorupski K. Overlapping binding sites for the virulence gene regulators AphA, AphB and cAMP-CRP at the Vibrio cholerae tcpPH promoter. Mol. Microbiol. 2001; 41(2):393– 407. DOI: 10.1046/j.1365-2958.2001.02518.x.
13. Lim B., Beyhan S., Meir J., Yildiz F.H. Cyclic-diGMP signal transduction systems in Vibrio cholerae: modulation of rugosity and biofilm formation. Mol. Microbiol. 2006; 60(2):331–48. DOI: 10.1111/j.1365-2958.2006.05106.x.
14. Lipfert J., Das R., Chu V.B., Kudaravalli M., Boyd N., Herschlag D., Doniach S. Structural transitions and thermodynamics of a glycine-dependent riboswitch from Vibrio cholerae. J. Mol. Biol. 2007; 365(5):1393–406. DOI: 10.1016/j.jmb.2006.10.022.
15. Liu J.M., Camilli A. A broadening world of bacterial small RNAs. Curr. Opin. Microbiol. 2010; 13(1):18–23. DOI: 10.1016/j.mib.2009.11.004.
16. Liu J.M., Camilli A. Discovery of bacterial sRNAs by high- throughput sequencing. Methods Mol. Biol. 2011; 733:63–79. DOI: 10.1007/978-1-61779-089-8_5.
17. Mandal M., Lee M., Barrick J.E., Weinberg Z., Emilsson G.M., Ruzzo W.L., Breaker R.R. A glycine-dependent riboswitch that uses cooperative binding to control gene expression. Science. 2004; 306(5694):275–9. DOI: 10.1126/science.1100829.
18. Massé E., Majdalani N., Gottesman S. Regulatory roles for small RNAs in bacteria. Curr. Opin. Microbiol. 2003; 6(2):120–4. DOI: 10.1016/S1369-5274(03)00027.
19. Narberhaus F., Vogel J. Regulatory RNAs in prokaryotes: here, there and everywhere. Mol. Microbiol. 2009; 74(2):261–9. DOI: 10.1111/j.1365-2958.2009.06869.x.
20. Repoila F., Darfeuille F. Small regulatory non-coding RNAs in bacteria: physiology and mechanistic aspects. Biol. Cell. 2009; 101(2):117–31. DOI: 10.1042/BC20070137.
21. Shao Y., Bassler B. L. Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae. Mol. Microbiol. 2014; 92(5):921–30. DOI: 10.1111/mmi.12599.
22. Smith K.D., Lipchock S.V., Ames T.D., Wang J., Breaker R.R., Strobel S.A. Structural basis of ligand binding by a c-di-GMP riboswitch. Nat. Struct. Mol. Biol. 2009; 16(12):1218–23. DOI: 10.1038/nsmb.1702.
23. Song T., Mika F, Lindmark B., Liu Z., Schild S., Bishop A., Zhu J., Camilli A., Johansson J., Vogel J., Wai S.N. A new Vibrio cholerae RNA modulates colonization and affects release of outer membrane vesicles. Mol. Microbiol. 2008; 70(1):100–11. DOI: 10.1111/j.1365-2958.2008.06392.x.
24. Waters L.S., Storz G. Regulatory RNAs in bacteria. Cell. 2009; 136(4):615–28. DOI: 10.1016/j.cell.2009.01.043.
25. Yamamoto S., Izumiya H., Mitobe J., Morita M., Arakawa E., Ohnishi M., Watanabe H. Identification of a chitin-induced small RNA that regulates translation of the tfoX gene, encoding a positive regulator of natural competence in Vibrio cholerae. J. Bacteriol. 2011; 193(8):1953–65. DOI: 10.1128/JB.01340-10.
Review
For citations:
Pisanov R.V., Vodop’yanov A.S., Simakova D.I. THE ROLE OF SMALL RNAS IN CONTROLLING EXPRESSION OF GENES INVOLVED IN THE IMPLEMENTATION OF VIBRIO CHOLERAE PATHOGENICITY. Problems of Particularly Dangerous Infections. 2017;(2):36-39. (In Russ.) https://doi.org/10.21055/0370-1069-2017-2-36-39
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