LIQUID NUTRIENT MEDIUM FOR SUBMERGED CULTIVATION OF TULAREMIA MICROBE
https://doi.org/10.21055/0370-1069-2017-2-81-83
Abstract
The paper describes an effective liquid nutrient medium, utilization of which in the process of submerged cultivation of the vaccine tularemia microbe strain allows for the production of high concentrations of viable biomass with low rates of dissociation, which is essential in manufacturing of live vaccines. Dry enzymatic hydrolysate of fibrin, by-product of anti-rabies immunoglobulin production is used as a nutrient-rich base of the new nutrient medium.
About the Authors
O. A. Volokh
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
M. V. Antonycheva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
N. G. Avdeeva
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
E. M. Kuznetsova
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
K. I. Kholmatov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
D. N. Bibikov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
A. K. Nikiforov
Russian Research Anti-Plague Institute “Microbe”
Russian Federation
46, Universitetskaya St., Saratov, 410005
Russian Federation
46, Universitetskaya St., Saratov, 410005
References
1. Antonycheva M.V., Nizhegorodtsev S.A., Eremin S.A., Alenkina T.A., Shul’gina I.V., Belousov A.D., Zhulidov I.M., Nikiforov A.K. [Nutrient medium for submerged cultivation of cholera viobrio]. RF Patent No 2425866, 10.08.2011.
2. Volokh O.A., Antonycheva M.V., Avdeeva N.G., Vakhrushina N.I., Nikiforov A.K. [Nutrient medium for submerged cultivation of tularemia microbe]. RF Patent No 2518282, 10.06.2014.
3. Lapin A.A., Pavlov V.M., Mokrievich A.N., Domotenko L.V., Khramov M.V. [Simple liquid nutrient medium for molecular-genetic investigations of Francisella tularensis]. Probl. Osobo Opasn. Infek. 2009; 102:66–7.
4. Pavlovich N.V., Mishan’kin B.N. [Transparent nutrient medium for Francisella tularensis cultivation]. Antibiotiki i Med. Biotekhnol. 1987; 32:133–7.
5. Shepelev I.A., Volokh O.A., Eremin S.A., Avdeeva N.G., Kuznetsova E.M. [Method for the production of biomass of tularemia microbe]. RF Patent No 2451743, 27.05.2012.
6. Becker S., Lochau P., Jacob D., Heuner K., Grunow R. Successful re-evaluation of broth medium T for growth of Francisella tularensis ssp. and other highly pathogenic bacteria. J. Microbiol. Methods. 2016; 121:5–7. DOI: 10.1016/j.mimet.2015.11.018.
Review
For citations:
Volokh O.A., Antonycheva M.V., Avdeeva N.G., Kuznetsova E.M., Kholmatov K.I., Bibikov D.N., Nikiforov A.K. LIQUID NUTRIENT MEDIUM FOR SUBMERGED CULTIVATION OF TULAREMIA MICROBE. Problems of Particularly Dangerous Infections. 2017;(2):81-83. (In Russ.) https://doi.org/10.21055/0370-1069-2017-2-81-83
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