Study of the UV Irradiation and Nalidicsic Acid Effect on the RecA-protein Induction in Francisella tularensis 15/10 Cells
https://doi.org/10.21055/0370-1069-2011-3(109)-36-39
Abstract
Studied is the UV irradiation and nalidicsic acid effect on the RecA-protein synthesis in Francisella tularensis 15/10 cells. Obtained is the specific murine serum to the recombinant RecA-protein. The results of immunoblotting with this specific serum demonstrate that the amount of RecA-protein in Francisella tularensis 15/10 cells is not increased after the exposure to these damaging factors.
Keywords
616.981.455
About the Authors
A. A. Lapin
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
T. B. Kravchenko
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. N. Mokrievich
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
G. M. Vakhrameeva
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
T. I. Kombarova
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
I. A. Dyatlov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
V. M. Pavlov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
References
1. Lapin A.A., Pavlov V.M., Mokrievich A.N., Domotenko L.V., Khramov M.V. [Simple liquid nutrient medium for molecular-genetic investigations of Francisella tularensis]. Probl. Osobo Opasn. Infek. 2009; 4(102):66–7.
2. Maniatis T., Frich E., Sembruk G., [Methods of Genetic Engineering. Molecular Cloning]. Moscow: Izd. “Mir”; 1984. 480 p.
3. Fischer W., Haas R. The RecA protein of Helicobacter pylori requires a posttranslational modification for full activity. J. Bacteriol. 2004; 186(3):777-84
4. Kowalczykowski S.C., Dixon D.A., Eggleston A.K., Lauder S.D., Rehrauer W.M. Biochemistry of homologous recombination in Escherichia coli. Microbiol. Rev. 1994; 58(3):401-65
5. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature(London). 1970; 227:680-685
6. Lowry O.H., Rosenbrough N.R., Farr A.L., Randall RJ. Protein measurement with the folin phenol. J. Biol. Chem. 1951; 193:115-9.
7. Miller J.H. Experiments in Molecular Genetics. N.Y.: Gold Harbor Laboratory; 1972.
8. Ngo T.T., Lenhoff H.M., editor. Enzyme-mediated immunoassay. New York, London: Plenum Press; 1988. 444 p.
9. Papavinasasundaram K.G., Anderson C., Brooks P.C., Thomas N.A., Movahedzadeh F., Jenner P.J. et al. Slow induction of RecA by DNA damage in Mycobacterium tuberculosis. Microbiology. 2001; 47:3271-9.
10. Towbin H., Staenelin T., Gordon J. Electroforetic transfer of proteins from polyacrilamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 1979; 76(9):4350-4.
11. Weinstock G.M., McEntee K.M., Lehman I.R. ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli. Proc. Natl. Acad. Sci. USA. 1979; 76(1):126-30.
Review
For citations:
Lapin A.A., Kravchenko T.B., Mokrievich A.N., Vakhrameeva G.M., Kombarova T.I., Dyatlov I.A., Pavlov V.M. Study of the UV Irradiation and Nalidicsic Acid Effect on the RecA-protein Induction in Francisella tularensis 15/10 Cells. Problems of Particularly Dangerous Infections. 2011;(3(109)):36-39. (In Russ.) https://doi.org/10.21055/0370-1069-2011-3(109)-36-39
Views:
476
Email this article 