Obtainment of the Immunomagnetic Particles for Francisella tularensis Cells Detection
https://doi.org/10.21055/0370-1069-2011-3(109)-50-53
Abstract
Obtained are the immunomagnetic particles with the immobilized highly-specific monoclonal antibodies FB 11-x to Francisella tularensis. Their usage in enzyme-linked immunoassays made it possible to detect 105 - 106 CFU/ml of F. tularensis. The binding of the bacterial cells with IMP was confirmed by the results of fluorescent microscopy.
Keywords
Francisella tularensis,
immunomagnetic particles,
monoclonal antibodies,
enzyme-linked immunoassay,
fluorescent microscopy
616.981.455:616-097
About the Authors
S. S. Vetchinin
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
P. Kh. Kopylov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
N. V. Kiseleva
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
O. G. Nikolaeva
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. V. Gavryushkin
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
References
1. Belyi V.A., Pinchuk L.S. [Introduction to the Material Engineering of Sealing Systems]. Minsk: Nauka i Tekhnika; 1980. 169 p.
2. Egorov A.M., Osipov A.P., Dzantiev B.B., Gavrilova E.M. [Theory and practice of enzyme-linked immunoassay]. M.: Vys. Shkola; 1991. 288 p.
3. Khlebnikov V.S., Golovlev I.R., Tokhtamysheva N.V., Averin S.F., Kulevatsky D.P., Averina A.A. et al. [Epitope detection of the preventive mono- clonal antibodies, specific for Francisella tularensis lipopolyssacharide]. Zh. Mikrobiol. Epidemiol. Immunobiol. 1993; 1:83–8.
4. Shlyapnikova E.A., Shlyapnikov Yu.M., Afanas’ev V.N., Afanas’eva G.V., Gavryushkin A.V., Beletsky I.P. [Examination of the quality of amino- modified bases, used for the hybridization analysis]. Bioorganic Chemistry. 2007; 33(2):261–8.
5. Goding J.W. Conjugation of antibodies with fluorochrome: modifications to the standard methods. J. Immunol. Meth. 1976; 13:215-26.
6. Grant I.R., Ball H.J., Rowe1 M.T. Isolation of Mycobacterium paratuberculosis from milk by immunomagnetic separation. Appl. Environ. Microbiol. 1998; 64(9):3153-8.
7. Hofmann K., Finn F.M., Kiso Y. Avidin-biotin affinity columns. General methods for attaching biotin to peptides and proteins. J. Amer. Chem. Soc. 1978; 100(11):3585-90.
8. Khlebnikov V.S., Vetchinin S.S., Tochtamycheva N.V., Golovljov I.R., Grechko G.K., Kulevatsky D.P. et al. Preventive of monoclonal antibodies specific to LPS Fracisella tularensis and localisation of antigenic determinant. 11th Meeting Europ. Federat. Immunol. Soc. Abstracts. Finland; 1991. P. 23-4.
9. Lvov Y., Mohwald H., Dekker M., editors. Protein architecture: interfacing molecular assemblies and immobilization biotechnology. New York; 2000. P. 125-67.
10. Massart R. Preparation of aqueous magnetic liquids in alkaline and acidic media. IEEE Trans. Magn. 1981; 2:1247-8.
11. Petrov A.I., Gavryushkin A.V., Sukhorukov G.B. Effect of temperature, pH and shell thickness on the rate of Mg2+ and Ox2- release from multilayered polyelectrolyte shells deposited onto microcrystals of magnesium oxalate. J. Phys. Chem. B. 2003; 107:868-75.
12. Pyle B.H., Broadaway S.C., McFeters G.A. Sensitive detection of Escherichia coli O157:H7 in food and water by immunomagnetic separation and solid-phase laser cytometry. Appl. Environ. Microbiol. 1999; 65(5):1966-72.
13. Wilson M.B., Nakane P.P. Recent developments in the periodate method of conjugating horseradish peroxidase (HRPO) to antibodies, in immunofluorescence and related staining techniques. In: Knapp W., Holubar K., Wick G., editors. Immunofluorescence and Related Staining Techniques. Elsevier/North Holland Biomedical, Amsterdam; 1978. P. 215-24.
Review
For citations:
Vetchinin S.S., Kopylov P.Kh., Kiseleva N.V., Nikolaeva O.G., Gavryushkin A.V. Obtainment of the Immunomagnetic Particles for Francisella tularensis Cells Detection. Problems of Particularly Dangerous Infections. 2011;(3(109)):50-53. (In Russ.) https://doi.org/10.21055/0370-1069-2011-3(109)-50-53
Views:
453
Email this article 