Assessment of the New Set of Reagents AmpliSens Brucella spp.-FL with Hybridization-Fluorescent Detection
https://doi.org/10.21055/0370-1069-2011-3(109)-58-60
Abstract
At CRIE, Moscow and RRAPI Microbe designed is the set of reagents for detection of Brucella spp. DNA in biological materials and cultures of microorganisms by means of PCR with hybridization-fluorescent registration of the results. This reagent set, AmpliSens ® Brrucella spp.-FL, is supplied in two modifications versus the mechanism of the results registration (FRT - real time and FEP - end-point). High diagnostic value of the reagent set is determined in medical trials at L.A. Tarasevich Institute. The set is registered as medical device.
Keywords
brucellosis,
Brucella spp.,
PCR,
hybridization-fluorescent registration of the results,
specific activity,
specificity,
reproducibility
616.981.42
About the Authors
A. N. Malakhaeva
L.A.Tarasevich State Research Institute for Standardization and Control
Russian Federation
Russian Federation
L. V. Sayapina
L.A.Tarasevich State Research Institute for Standardization and Control
Russian Federation
Russian Federation
I. S. Barulina
L.A.Tarasevich State Research Institute for Standardization and Control
Russian Federation
Russian Federation
I. V. Kasina
L.A.Tarasevich State Research Institute for Standardization and Control
Russian Federation
Russian Federation
A. S. Abdrashitova
L.A.Tarasevich State Research Institute for Standardization and Control
Russian Federation
Russian Federation
N. A. Osina
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
I. N. Sharova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
S. B. Yatsyshina
Central Research Institute of Epidemiology
Russian Federation
Russian Federation
N. V. Mayorov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
L. V. Zaytseva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. P. Mironova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
References
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Review
For citations:
Malakhaeva A.N., Sayapina L.V., Barulina I.S., Kasina I.V., Abdrashitova A.S., Osina N.A., Sharova I.N., Yatsyshina S.B., Mayorov N.V., Zaytseva L.V., Mironova N.P. Assessment of the New Set of Reagents AmpliSens Brucella spp.-FL with Hybridization-Fluorescent Detection. Problems of Particularly Dangerous Infections. 2011;(3(109)):58-60. (In Russ.) https://doi.org/10.21055/0370-1069-2011-3(109)-58-60
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