Development of the Multiplex Real-Time PCR for Marburg, Ebola, and Lassa Viruses Identification
https://doi.org/10.21055/0370-1069-2011-3(109)-64-67
Abstract
Presented are the data on the development and approbation of the method of Marburg, Ebola, and Lassa viruses identification based on real-time multiplex PCR with hybridization-fluorescent detection. This method is meant for the differential diagnostics of hemorrhagic fevers caused by these viruses. Displayed are the results of determination of multiplex PCR analytical sensitivity and specific activity.
About the Authors
A. O. Sementsova
State Research Centre of Virology and Biotechnology Vector
Russian Federation
Russian Federation
A. N. Shikov
State Research Centre of Virology and Biotechnology Vector
Russian Federation
Russian Federation
V. A. Ternovoy
State Research Centre of Virology and Biotechnology Vector
Russian Federation
Russian Federation
A. V. Vinokurova
State Research Centre of Virology and Biotechnology Vector
Russian Federation
Russian Federation
N. E. Kostina
State Research Centre of Virology and Biotechnology Vector
Russian Federation
Russian Federation
E. A. Stavsky
State Research Centre of Virology and Biotechnology Vector
Russian Federation
Russian Federation
A. P. Agafonov
State Research Centre of Virology and Biotechnology Vector
Russian Federation
Russian Federation
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Review
For citations:
Sementsova A.O., Shikov A.N., Ternovoy V.A., Vinokurova A.V., Kostina N.E., Stavsky E.A., Agafonov A.P. Development of the Multiplex Real-Time PCR for Marburg, Ebola, and Lassa Viruses Identification. Problems of Particularly Dangerous Infections. 2011;(3(109)):64-67. (In Russ.) https://doi.org/10.21055/0370-1069-2011-3(109)-64-67
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