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Development of the Multi-Analyte Test for Immune-Chromatographic Detection of Botulinum Toxins

https://doi.org/10.21055/0370-1069-2011-3(109)-68-70

Abstract

Designed is the multi-analyte test for simultaneous immune chromatographic detection of A & B type botulinum neurotoxins (BT), using colloid gold nanoparticles. It is meant for food and environmental samples' analysis. The sensitivity of simultaneous BT detection of the A (30 ng/ml) and B (10 ng/ml) types is as high as that of mono-analytical tests, designed for one type BT detection. The test is demonstrated to be a specific one and can be used for BT detection in food stuffs.

About the Authors

I. V. Shilenko
State Research Institute of Biological Device Engineering
Russian Federation


A. A. Titov
State Research Institute of Biological Device Engineering
Russian Federation


S. P. Yarkov
State Research Institute of Biological Device Engineering
Russian Federation


V. N. Zlobin
State Research Institute of Biological Device Engineering
Russian Federation


References

1. Pozdeev O.K. [Medical Microbiology]. M.: GEOTAR-Media; 2007. 768 p.

2. Rakova I.D., Gurzhey S.N., Smetanin N.N. [About the botulism diag- nostic techniques]. Med. Ekstrem. Sit. 2010; 33(3):71–3.

3. Arnon S.S., Schechter R., Inglesby T.V., Henderson D.A., Bartlett J.G., Ascher M.S. et al. Botulinum toxin as a biological weapon: medical and public health management. JAMA. 2001; 285:1059-70.

4. Fach P., Gibert M., Griffais R., Guillou J.P., Popoff M. R. PCR and gene probe identification of botulinum neurotoxin A-, B-, E-, F-, and G-producing Clostridium spp. and evaluation in food samples. Appl. Environ. Microbiol. 1995; 61:389-92.

5. Ferreira J.L., Maslanka S., Johnson E, Goodnough M. Detection of botulinal neurotoxins A, B, E, and F by amplified enzyme-linked immunosorbent assay: collaborative study. J. AOAC Int. 2003; 86:314-31.

6. Frens G. Preparation of gold dispersions of varying particle size: Controlled nucleation or the regulation of the particle size in monodisperse gold suspensions. Nat. Phys. Sci. 1973; 241(1):20-2.

7. Hermanson G.T. Bioconjugate Techniques. Academic Press; 2008. P. 924-34.

8. Horisberger M., Rosset J. Colloidal gold, a useful marker for transmission and scanning electron microscopy. J. Histochem. Cytochem. 1977; 25(4):295-305.

9. Geoghegan W.D., Ackerman G.A. Adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concavalin A, wheat germ agglutinin and goat anti-human immunoglobulin G on cell surfaces at the electron microscopic level: a new method, theory and application. J. Histochem. Cytochem. 1977; 25:1187-200.

10. Niemann H. Molecular biology of the clostridial neurotoxins. In: Alouf J.E., Freer J.H., editors. A sourcebook on bacterial protein toxins. Academic Press, London, United Kingdom; 1991. P. 303-8.

11. Sharma S.K., Eblen B.S., Bull R.L., Burr D.H., Whiting R.C. Evaluation of lateral-flow Clostridium botulinum neurotoxin detection kits for food analysis. Appl. Environ. Microbiol. 2005; 71(7):3935-41.


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For citations:


Shilenko I.V., Titov A.A., Yarkov S.P., Zlobin V.N. Development of the Multi-Analyte Test for Immune-Chromatographic Detection of Botulinum Toxins. Problems of Particularly Dangerous Infections. 2011;(3(109)):68-70. (In Russ.) https://doi.org/10.21055/0370-1069-2011-3(109)-68-70

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ISSN 0370-1069 (Print)
ISSN 2658-719X (Online)