Optimization of the Process of Cholera Chemical Pelleted Vaccine Production Using Ultrafiltration Technology
https://doi.org/10.21055/0370-1069-2011-3(109)-71-74
Abstract
Technology of O-antigen obtaining from Vibrio cholerae M41production strain, using the hollow fiber ultrafiltration modules, is developed and introduced into cholera vaccine manufacturing. Application of ultrafiltration makes it possible to obtain native high-activity O-antigen preparations which comply with the current regulatory requirements. Besides, it provides for economy (10-15-fold) of ammonium sulphate, used for antigen precipitation, reduces the duration of technological cycle (2 times), and the labor inputs. The technology of protective antigen concentrating is tested on V. cholerae strains MO45 and KM137 of O139 serogroup. Three series of experimental V. cholerae O139 vaccine are obtained.
Keywords
616.932:615.371/372
About the Authors
O. V. Gromova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
S. A. Nizhegorodtsev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
I. A. Dyatlov
State Research Center for Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
V. V. Kutyrev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
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Review
For citations:
Gromova O.V., Nizhegorodtsev S.A., Dyatlov I.A., Kutyrev V.V. Optimization of the Process of Cholera Chemical Pelleted Vaccine Production Using Ultrafiltration Technology. Problems of Particularly Dangerous Infections. 2011;(3(109)):71-74. (In Russ.) https://doi.org/10.21055/0370-1069-2011-3(109)-71-74
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