Comparative Assessment of Yersinia pestis Strains on the Level of Plague Microbe Capsular Antigen Production
https://doi.org/10.21055/0370-1069-2011-3(109)-81-83
Abstract
Yersinia pestis recombinant strain KM277 (EV11MpFSK-3) Kmr is a potential producer-strain of capsular antigen (F1), since its productive properties and immunochemical activity of F1-antigen, synthesized by it, are higher than in Y. pestis EV and its derivatives. The apparent advantage of the Y. pestis KM277 strain is the ability to synthesize F1 antigen at 28 °C, and the absence of its own plague-microbe plasmids in cells. Worked out is the scheme of scaled cultivation of the recombinant strain and obtainment of capsular antigen preparation.
About the Authors
A. K. Nikiforov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
O. A. Volokh
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
S. A. Eremin
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
T. V. Alenkina
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
References
1. Anisimov A.P., Nikiforov A.K., Eremin S.A., Drozdov I.G. [Constructing of the Yersinia pestis strain with heightened (enhanced) protec- tive capacity]. Byul. Eksp. Biolog. Med. 1995; 11:27–31.
2. Veynblat V.I., Dal’vadyants S.N., Verenkov M.C. [Methods of isola- tion and purification of plague-agent capsular antigen and intracellular toxin]. Lab. Delo. 1983; 12:37–9.
3. Dal’vadyants S.N., Filippov A.F., Beloborodov R.A., Ogarev N.N. [Effectiveness of the complex Guiney-pig immunization by anti-plague F1 and basic O-antigen preparations]. Probl. Osobo Opasn. Infek. 1977; 55:105–12.
4. Dyatlov I.A., Filippov A.F., Bondarenko L.N. et al. [Application of the isogenic system of plague-agent strains in the process of antigen obtainment]. In: [Genetics, Microbiology, and Development of Methods of Laboratory Diagnostics of Particularly Dangerous Infections]. Saratov; 1991. P. 71–7.
5. Eremin S.A., Volokh O.A., Shepelev I.A. et al. [Developing of the new technological schemes, and scaling of the processes of plague and tularemia antigens’ obtainment ]. Probl. Osobo Opasn. Infek. 2006; 92:58–61.
6. Onishchenko G.G., Kutyrev V.V., editors. [Laboratory Diagnostics of Particularly Dangerous Infectious Diseases. Guidelines]. M.: Meditsina; 2009. P. 27–61.
7. Popov N.V., Kuklev E.V., Kutyrev V.V. [Tendencies of epizootic activ- ity of plague natural foci in the Russian Federation and CIS countries in early XXI century]. Epidem. Infek. Bol. 2006; 2:7–10.
8. Titenko M.M., Veynblat V.I., Verenkov M.C., Vasenin A.S. [Preparation method of isolation and purification of plague-agent capsular antigen by means of isoelectric precipitation]. In: [Diagnostics and Prophylaxis of Particularly Dangerous Infections]. Saratov; 1983. P. 34–9.
9. Baker E.E., Sommer H., Foster L.E. et al. Studies on immunization against plague. I. The isolation and characterization of the soluble antigen of Pasteurella pestis. J. Immunol. 1952; 68(2):131-45.
10. Li B.D., Zhou Z., Wang Z. et al. Antibody profiling in plague patients by protein microarray. Microbes Infect. 2008; 10:45-51.
Review
For citations:
Nikiforov A.K., Volokh O.A., Eremin S.A., Alenkina T.V. Comparative Assessment of Yersinia pestis Strains on the Level of Plague Microbe Capsular Antigen Production. Problems of Particularly Dangerous Infections. 2011;(3(109)):81-83. (In Russ.) https://doi.org/10.21055/0370-1069-2011-3(109)-81-83
Views:
576
Email this article 