Construction of the PCR Test-System for the Detection of Vibrio cholerae O1 Toxigenic Strains, for Indication of Their Biovar and for Differentiation between Typical and Altered El Tor-Vibrio Strains
https://doi.org/10.21055/0370-1069-2011-4(110)-49-52
Abstract
Worked out is the multilocus PCR test-system which makes it possible to identify Vibrio cholerae O1 strains on the basis of rfb gene detection, to determine their biovar - either Classical or El Tor by testing cas3 or rtxC genes respectively, and at the same time to differentiate them into typical (which carry ctxBEltor gene) and genetically altered (which carry ctxBClass gene) variants. Efficacy and specificity of the test-system is demonstrated by the analysis of 64 natural V. cholerae strains of various serogroups and biovars, and 13 strains of enterobacteria and other species of Vibrio genus. Relation of the studied V. cholerae El Tor isolates to the typical or altered vibrio variants is proved out by ctxB gene sequencing.
About the Authors
A. V. Shashkova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
A. A. Goryaev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
S. P. Zadnova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
Ya. M. Krasnov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. I. Smirnova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
V. V. Kutyrev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
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Review
For citations:
Shashkova A.V., Goryaev A.A., Zadnova S.P., Krasnov Ya.M., Smirnova N.I., Kutyrev V.V. Construction of the PCR Test-System for the Detection of Vibrio cholerae O1 Toxigenic Strains, for Indication of Their Biovar and for Differentiation between Typical and Altered El Tor-Vibrio Strains. Problems of Particularly Dangerous Infections. 2011;(4(110)):49-52. (In Russ.) https://doi.org/10.21055/0370-1069-2011-4(110)-49-52
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