Immunobiological Properties of Francisella tularensis 15/10 Strain with Deleted recA Gene
https://doi.org/10.21055/0370-1069-2011-4(110)-65-67
Abstract
Deletion of recA gene in Francisella tularensis 15/10 genome leads to the increase in its sensitivity to ultraviolet irradiation, reduction of the homologous recombination capacity, and a slight decline of virulence for mice. Efficacy of Francisella tularensis 15/10ΔrecA reproduction within microphage-like cells, its resistance to normal rabbit serum, and protective properties on the mouse model of tularemia are the same as in original Francisella tularensis 15/10.
Keywords
616.981.455:615.371/.372
About the Authors
A. A. Lapin
State Research Center of Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
A. N. Mokrievich
State Research Center of Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
G. M. Vakhrameeva
State Research Center of Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
T. I. Kombarova
State Research Center of Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
I. V. Bakhteeva
State Research Center of Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
I. A. Dyatlov
State Research Center of Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
V. M. Pavlov
State Research Center of Applied Microbiology and Biotechnology
Russian Federation
Russian Federation
References
1. Ashmarin I.P., Vorob’ev A.A. [Statistical Methods in Microbiological Investigations]. L.: Medgiz; 1962. 58 p.
2. Lapin A.A., Pavlov V.M., Mokrievich A.N., Domotenko L.V., Khramov M.V. [Simple liquid nutrient medium for molecular genetic investigations of Francisella tularensis]. Probl. Osobo Opasn. Infek. 2009; 4(102):66–7.
3. Maniatis T., Frich E., Sembruk G. [Methods of Genetic Engineering. Molecular Cloning.]. M.: Mir; 1964. 479 p.
4. Mokrievich A.N., Kondakova A.N., Valade E., Platonov M.E., Vakhrameeva M.E., Shaikhutdinova R.Z., Mironova R.I., Blakha D., Bakhteeva I.V., Titareva G.M., Kravchenko T.B., Kombarova T.I., Vidal’ D., Pavlov V.M., Lindner B., Dyatlov I.A., Knirel’ Yu.A. [Biological properties and lipopoly- saccharide structural organization of Francisella tularensis vaccine strain ob- tained after qseC gene inactivation]. Biokhimiya. 2010; 75(4):539–48.
5. Berg J.M., Mdluli K.E., Nano F.E. Molecular Cloning of the recA Gene and Construction of a recA Strain of Francisella novicida. Infect. and immun. 1992; 60(2): 690-3.
6. Golovliov I., Sjostedt A., Mokrievich A., Pavlov V. A method for allelic replacement in Francisella tularensis. FEMS Microbiol. Lett. 2003; 222:273-80.
7. Keller M., Boettger E.C., Sande P. Tuberculosis vaccine strain Mycobacterium bovis BCG Russia is a natural recA mutant. BMC Microbiol. 2008; 8:120.
8. Miller J.H. Experiments in Molecular Genetics. N.Y: Gold Harbor Laboratory. 1972. 436 р.
9. Roux C.M., Booth N.J., Bellaire B.H., Gee J.M., Roop R.M. II, Kovach M.E., Tsolis R.M., Elzer P.H., Ennis D.G. RecA and RadA Proteins of Brucella abortus Do Not Perform Overlapping Protective DNA Repair Functions following Oxidative Burst J. Bacteriol. 2006; 188(14):5187-95.
10. Sandstrom G. The tularemia vaccine. J. Chem. Tech. Biotech. 1994; 59:315-20.
11. Simon R., Priefer U., Puhler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology. 1983; 1:784-91.
12. Sjöstedt A., Sandström G., Tärnvik A., Jaurin B. Nucleotide sequence and T-cell epitopes of a membrane protein of Francisella tularensis. J. Immunol. 1990; 145:311-7.
13. Woodcock D.M., Crowther P.J., Doherty J., Jefferson S., DeCruz E., Noyer-Weidner M., Smith S.S., Michael M.Z., Graham M.W. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 1989; 17:3469-78.
Review
For citations:
Lapin A.A., Mokrievich A.N., Vakhrameeva G.M., Kombarova T.I., Bakhteeva I.V., Dyatlov I.A., Pavlov V.M. Immunobiological Properties of Francisella tularensis 15/10 Strain with Deleted recA Gene. Problems of Particularly Dangerous Infections. 2011;(4(110)):65-67. (In Russ.) https://doi.org/10.21055/0370-1069-2011-4(110)-65-67
Views:
452
Email this article 