Application of Bacillus anthracis Molecular Typing Methods by the Reference Center for the Anthrax Agent Monitoring
https://doi.org/10.21055/0370-1069-2011-4(110)-68-70
Abstract
Presented are the results of B. anthracis molecular typing of in the scope of work of the Reference center for the anthrax agent monitoring. Summarized is the experience of genotyping application in epidemiological investigation of anthrax outbreaks. MLVA-genotyping (8-25 VNTR-loci analysis) of B. anthracis strains, isolated in Russia and in neighboring regions is shown to provide the correct comparison of genotypes with the data of the global MLVA-data bank. The major tasks of reference center for the development of approaches to B. anthracis genotyping are identified. These approaches include research of additional genotyping methods such as SNP analysis, SNR analysis with assessment of the described loci variability and identification of new polymorphic regions.
About the Authors
A. G. Ryazanova
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
E. I. Eremenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
O. I. Tsygankova
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
E. A. Tsygankova
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
A. N. Kulichenko
Stavropol Research Anti-Plague Institute
Russian Federation
Russian Federation
References
1. Eremenko E.I., Ryazanova A.G., Tsygankova E.A., Tsygankova O.I., Kulichenko A.N. [Genotypic peculiarities of Bacillus anthracis strains with different manifestation of pathogenicity-associated features]. Probl. Osobo Opasn. Infek. 2010; 104:53–6.
2. [Arrangement of work of laboratories using the nucleic acid ampli- fication methods in the course of work with material containing microorgan- isms of pathogenicity groups I–IV. MU 1.3.2569-09]. M.; 2009.
3. Tsygankova O.I., Eremenko E.I., Bryukhanov A.F., Abgaryan A.G., EfremenkoV.I., Ryazanova A.G. [Gene typing of Bacillus anthracis strains isolated on the territory of the CIS countries]. Zh. Mikrobiol. Epidemiol. Immunobiol. 2003; 6(supplement):51–6.
4. Shishkova N.A., Mokrievich A.N., Platonov M.E., Svetoch T.E., Marinin L.I. [Study of the genetic diversity of the anthrax microbe strains from the collection of SRC AMB]. Probl. Osobo Opasn. Infek. 2010; 104:60–5.
5. Keim P., Price L.B., Klevytska A.M., Smith K.L., Schupp J.M., Okinaka R. et al. Multiple locus variable namber tandem repeat analysis reveals genetic relationships within Bacillus anthracis. J. Bacteriol. 2000; 182(10):2928-36.
6. Le Fleche P., Hauck Y., Onteniente L., Prieur A., Denoeud F., Ramisse V. et al. A tandem repeats database for bacterial genomes: application to the genotyping of Yersinia pestis and Bacillus anthracis. BMC Microbiology. 2001; 1:2.
7. Lista F., Faggioni G., Valjevac S., Ciammaruconi A., Vaissaire J., Doujet C. et al. Genotyping of Bacillus anthracis strains based on automated capillary 25-loci multiple locus variable-number tandem repeats analysis. BMC Microbiol. 2006; 6:33.
8. Stratilo C.W., Lewis C.T., Bryden L., Mulvey M.R., Bader D. Single-nucleotide repeat analysis for subtyping Bacillus anthracis isolates. J. Clin. Microbiol. 2006; 44(3):777-82.
9. Van Ert M.N., Easterday W.R., Huynh L.Y. et al. Global Genetic Population Structure of Bacillus anthracis. PLoS ONE. 2007; 2(5):e461.
Review
For citations:
Ryazanova A.G., Eremenko E.I., Tsygankova O.I., Tsygankova E.A., Kulichenko A.N. Application of Bacillus anthracis Molecular Typing Methods by the Reference Center for the Anthrax Agent Monitoring. Problems of Particularly Dangerous Infections. 2011;(4(110)):68-70. (In Russ.) https://doi.org/10.21055/0370-1069-2011-4(110)-68-70
Views:
597
Email this article 