Identification and Intraspecific Typing of Plague Microbe Strains with Their Potential Virulence Determination Using PCR
https://doi.org/10.21055/0370-1069-2011-2(108)-36-41
Abstract
Developed is the method of identification and intraspecific typing of plague microbe strains along with their potential virulence determination. Intraspecific differentiation and focal attribution of the examined plague microbe strains can be determined by monolocus VNTR-PCR, and main virulence determinants (chromosomal pigmentation region and calcium-dependence plasmid genes) - by multiplex PCR.
Keywords
616.981.452:576.8.097.21
About the Authors
A. V. Gaeva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
E. G. Boolgakova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
I. Yu. Sukhonosov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
L. V. Anisimova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
L. A. Novichkova
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
V. V. Kutyrev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
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Review
For citations:
Gaeva A.V., Boolgakova E.G., Sukhonosov I.Yu., Anisimova L.V., Novichkova L.A., Kutyrev V.V. Identification and Intraspecific Typing of Plague Microbe Strains with Their Potential Virulence Determination Using PCR. Problems of Particularly Dangerous Infections. 2011;(2(108)):36-41. (In Russ.) https://doi.org/10.21055/0370-1069-2011-2(108)-36-41
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