Variability of phoP/phoQ and rovA Genes Sequences - Global Regulators of the Plague Agent Life Span
https://doi.org/10.21055/0370-1069-2012-2(112)-25-28
Abstract
Compared are the gene sequences of the global phoP/phoQ and rovA transcription regulators in original Yersinia pestis strains of different subtypes. Detected is overall identity of the sequenced phoQ fragment and high conservatism of the rovA gene. All the plague agent strains belonging to the main subspecies have a missense mutation in phoP gene. It is a substitution of a single nucleotide G→A in the position 643 from the beginning of the gene, which causes amino-acid residue shift Gly→Ser in the position 215 in polypeptide chain of the PhopP protein, and, is a possible cause of alteration of the PhoP transcription activity in Yersinia pestis strains belonging to the main subspecies.
About the Authors
G. A. Eroshenko
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
E. I. Koshel'
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
G. N. Odinokov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. Yu. Shavina
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
Ya. M. Krasnov
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
N. P. Guseva
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
V. V. Kutyrev
Russian Research Anti-Plague Institute Microbe
Russian Federation
Russian Federation
References
1. Bazanova L.P., Innokent’eva T.I. [Concerning the role of fleas – primary and secondary vectors of plague – for the agent circulation in the Siberian natural foci]. Med. Parazitol. Parazitarn. Bol. 2008; 3:54–60.
2. Kutyrev V.V., Konnov N.P., Volkov Yu.P. [Plague Agent: Ultra- structure and Localization in the Vector]. M.: Meditsina; 2007. 222 p.
3. Maniatis T., Frich E., Sembruk G. [Molecular Cloning]. M.: Mir; 1984. 480 p.
4. Bacot A.W., Martin C.J. Observation on the mechanism of the transmission of plague by fleas. J. Hyg. (Lond). 1914; 13 (Suppl.):423-39.
5. Cathelyn J.S., Grosby S.D., Lathem W.W., Goldman W.E., Miller V.l. RovA - a global regulator of Yersinia pestis, specifically required for bubonic plague. Proc. Natl. Acad. Sci. USA. 2006; 103:13514-19.
6. Eroshenko G.A., Vidyaeva N.A., Kutyrev V.V. Comparative analysis of biofilm formation by main and nonmain subspecies of Yersinia pestis strains. FEMS Immunol. Med. Microbiol. 2010; 59:513-20.
7. Grabenstein J.P., Fukuto H.S., Palmer L.E., Bliska J.B. Characterization of phagosome trafficking and identification of PhoP-regulated genes important for survival of Yersinia pestis in macrophages. Infect. Immun. 2006; 74:3727-41.
8. Hitchen P.G., Prior G.L., Oyston P.C., Panico M., Wren B.W., Titball R.W. et al. Structural characterization of lypo-oligosaccharide (LOS) from Yersinia pestis: regulation of LOS structure by the PhoPQ system. Mol. Microbiol. 2002; 44:637-50.
9. Oyston P.C.F., Dorell N., Williams K., Li S.-R., Green M., Titball R.W., Wren B.W. The response regulator PhoP is important for survival under conditions of macrophage-induced stress and virulence in Yersinia pestis. Infect. Immun. 2000; 68:3419-25.
10. Sanger F., Nicklen L., Coulson A. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 1977; 74:5463-7.
11. Tran H.J., Heroven A.K., Winkler L., Spreter T., Beatrix B., Dersch P. Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation. J. Biol. Chem. 2005; 280:42423-32.
12. Vadivaloo V.V. PhoP regulation of Yersinia pestis during flea infection. In: Yersinia 2010. 10th Intern. Symp.; 2010 Oct 23-27. Brazil; 2010. P. 67.
13. Zhou D., Han Y., Qin I., Chen Z., Qin J., Song Y. et al. Transcriptome analysis of the Mg2+-responsive PhoP regulator in Yersinia pestis. FEMS Microbiol. Lett. 2005; 250:85-95.
Review
For citations:
Eroshenko G.A., Koshel' E.I., Odinokov G.N., Shavina N.Yu., Krasnov Ya.M., Guseva N.P., Kutyrev V.V. Variability of phoP/phoQ and rovA Genes Sequences - Global Regulators of the Plague Agent Life Span. Problems of Particularly Dangerous Infections. 2012;(2(112)):25-28. (In Russ.) https://doi.org/10.21055/0370-1069-2012-2(112)-25-28
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