Cloning and Expression of ace Gene of Vibrio cholerae in Escherichia coli
https://doi.org/10.21055/0370-1069-2012-2(112)-39-42
Abstract
Accessory cholera enterotoxin (ace) gene of Vibrio cholerae, amplified by PCR, is cloned into the BamHI-HindIII sites using plasmid pQE30. Expression of ace gene is under the control of T5 promotor. The Escherichia coli strain M15[pREP4]pAce90 (KM 194), containing recombinant plasmid, is an active producer of 6His-Ace protein, which possesses biological activity in the models of suckling mice and causes accumulation of fluid in the intestine. The product is contained in inclusion bodies found in the cells of coliform bacterium. The engineered producer strain can be used to obtain Ace preparations with a view to study its significance as pathogenicity factor in cholera vibrio.
Keywords
616.932:575
About the Authors
E. V. Monakhova
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
R. V. Pisanov
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
L. M. Verkina
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
A. V. Mironova
Rostov-on-Don Research Anti-Plague Institute
Russian Federation
Russian Federation
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Review
For citations:
Monakhova E.V., Pisanov R.V., Verkina L.M., Mironova A.V. Cloning and Expression of ace Gene of Vibrio cholerae in Escherichia coli. Problems of Particularly Dangerous Infections. 2012;(2(112)):39-42. (In Russ.) https://doi.org/10.21055/0370-1069-2012-2(112)-39-42
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