Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of Burkholderia
https://doi.org/10.21055/0370-1069-2012-2(112)-59-61
Abstract
The set of oligonucleotide primers was designed to identify β-lactam-resitance determinats in isolates of pathogenic Burkholderia using PCR. Simultaneously identified was certain molecular class of detected β-lactamases. PCR with primers specific to metallo-β-lactamase (class B) and oxacillinase of D-ala carboxypeptidase family (class D of β-lactamase) gene sequences allowed to differentiate among pseudomallei group of Burkholderia species.
Keywords
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About the Authors
A. V. Romanova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
I. B. Zakharova
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
V. S. Zamaraev
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
D. V. Viktorov
Volgograd Research Anti-Plague Institute
Russian Federation
Russian Federation
References
1. Ambler R. The structure of β-lactamases. Philos. Trans. R. Soc. Lond. (Biol.) 1980; 289:321-31.
2. Chaowagul W. Recent advances in the treatment of severe melioidosis. Acta Trop. 2000; 74:133-7.
3. Cheung T., Ho P., Woo P., Yuen K., Chau P. Cloning and expression of class A β-lactamase gene blaABPS in Burkholderia pseudomallei. Antimicrob. Agents Chemother. 2002; 46:1132 -5.
4. Dance D.A., Wuthiekanun V., Chaowagul W., Suputtamongkol Y., White N.J. Development of resistance to ceftazidime and co-amoxiclav in Pseudomonas pseudomallei. J. Antimicrob. Chemother. 1991; 28:321-4.
5. Dance D.A., Wuthiekanun V., Chaowagul W., White N.J. The antimicrobial susceptibility of Pseudomonas pseudomallei. Emergence of resistance in vitro and during treatment. Antimicrob. Chemother. 1989; 24:295-309.
6. Ho P., Cheung T., Yam W., Yuen K. Characterization of a laboratory-generated variant of BPS β-lactamase from Burkholderia pseudomallei that hydrolyses ceftazidime. Antimicrob. Chemother. 2002; 250:723-6.
7. Holden M.T., Titball R.W., Peacock S.J., Cerdeno-Tarraga A.M., Atkins T. et al. Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Proc. Natl Acad. Sci USA. 2004; 101:14240-5.
8. Nierman W.C., DeShazer D., Kim H.S., Tettelin H., Nelson K.E. Structural flexibility in the Burkholderia mallei genome. Proc. Natl Acad. Sci USA. 2004; 101:14246-51.
9. Niumsup P., Wuthiekanun V. Cloning of the class D β-lactamase gene from Burkholderia pseudomallei and studies on its expression in ceftazidime-susceptible and resistant strains. J. Antimicrob. Chemother. 2002; 50:445-55.
10. Thompson J., Higgins D., Gibson T. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22:4673-80.
11. Tribuddharat C., Moore R.A., Baker P., Woods D.E. Burkholderia pseudomallei class A beta-lactamase mutations that confer selective resistance against ceftazidime or clavulanic acid inhibition. Antimicrob. Agents Chemother. 2003; 47:2082-7.
12. White N.J., Dance D.A., Chaowagul W., Wattanagoon Y., Wuthiekanun V., Pitakwatchara N. Halving of mortality of severe melioidosis by ceftazidime. Lancet. 1989; 2(8665):697-701.
Review
For citations:
Romanova A.V., Zakharova I.B., Zamaraev V.S., Viktorov D.V. Design of Primers for Detection and Typing of β-Lactamase Genes from Pathogenic Species of Burkholderia. Problems of Particularly Dangerous Infections. 2012;(2(112)):59-61. (In Russ.) https://doi.org/10.21055/0370-1069-2012-2(112)-59-61
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